A5032450286- CRISPR and Genetic Engineering
- Retinal Diseases and Treatments
- Virus-based gene therapy research
- Retinal Imaging and Analysis
- Chromosomal and Genetic Variations
- RNA Research and Splicing
- Mesenchymal stem cell research
- Cancer-related molecular mechanisms research
- Zebrafish Biomedical Research Applications
- RNA Interference and Gene Delivery
- Viral Infectious Diseases and Gene Expression in Insects
- FOXO transcription factor regulation
- Immune Cell Function and Interaction
- Retinal Development and Disorders
- Hematopoietic Stem Cell Transplantation
- Glaucoma and retinal disorders
Singapore National Eye Center
2017-2020
Singapore Eye Research Institute
2017-2020
Inserm
2014-2017
Institut Cochin
2013-2017
Sorbonne Paris Cité
2017
Université Paris Cité
2014-2017
Délégation Paris 5
2017
Centre National de la Recherche Scientifique
2014
Abstract Background Despite increasing demand, current protocols for human pluripotent stem cell (hPSC)-derived retinal pigment epithelium (RPE) remain time, labor, and cost intensive. Additionally, absence of robust methods selective RPE purification removal non-RPE impurities prevents upscaling clinical quality production. We aimed to address these challenges by developing a simplified hPSC-derived production system that yields high-quality monolayers within 90 days. Methods Human cells...
Understanding the role of Notch and its ligands within different bone marrow niches could shed light on mechanisms regulating haematopoietic progenitor cells (HPCs) maintenance self-renewal. Here, we report that murine HPCs activation by vascular Delta-4 ligand maintains a significant proportion specifically in G0 state. Furthermore, Delta-4/Notch pathway limits significantly loss vivo short-term reconstitutive potential upon transplantation activated into lethally irradiated recipient mice....