A5103162478- Retinal Development and Disorders
- Pluripotent Stem Cells Research
- Retinal and Optic Conditions
- Cerebral Venous Sinus Thrombosis
- Glaucoma and retinal disorders
- Photochromic and Fluorescence Chemistry
- Ophthalmology and Visual Health Research
- Neurogenesis and neuroplasticity mechanisms
- Neuroscience and Neuropharmacology Research
- Corneal Surgery and Treatments
- Retinal Imaging and Analysis
- CRISPR and Genetic Engineering
- Tissue Engineering and Regenerative Medicine
- Retinal Diseases and Treatments
- Photoreceptor and optogenetics research
- Sentiment Analysis and Opinion Mining
- Ubiquitin and proteasome pathways
- Renal and related cancers
- Mesenchymal stem cell research
- Ocular Disorders and Treatments
- Ophthalmology and Eye Disorders
- Glioma Diagnosis and Treatment
- Advanced Text Analysis Techniques
- Multiple Sclerosis Research Studies
- Systemic Lupus Erythematosus Research
Doon University
2023-2025
Sarojini Naidu Medical College
2025
Indira Gandhi Delhi Technical University for Women
2024
Singapore National Eye Center
2013-2023
The Australian Prevention Partnership Centre
2023
Duke-NUS Medical School
2015-2022
Singapore Eye Research Institute
2013-2022
Subharti Medical College
2021
Institute of High Performance Computing
2021
Agency for Science, Technology and Research
2021
Nonophthalmologist physicians do not confidently perform direct ophthalmoscopy. The use of artificial intelligence to detect papilledema and other optic-disk abnormalities from fundus photographs has been well studied.We trained, validated, externally tested a deep-learning system classify optic disks as being normal or having 15,846 retrospectively collected ocular that had obtained with pharmacologic pupillary dilation various digital cameras in persons multiple ethnic populations. Of...
A BSTRACT Background and Objective: The COVID-19 disease caused by SARS-CoV-2 was declared as pandemic WHO soon after its emergence in 2019. This virus known to cause serious clinical symptoms severe illness. By using RT-PCR, which reports the cycle threshold value, is diagnosed, whereas for severity of disease, biomarker levels, like IL-6, CRP, D-dimer, serum ferritin procalcitonin, can be measured. We, thus, aimed explore any potential correlation value level with outcome COVID-19-positive...
Abstract Growing evidence suggests that glial cells may have a role as neural precursors in the adult central nervous system. Although it has been shown Müller exhibit progenitor characteristics postnatal chick and rat retinae, their progenitor-like developed human retina is unknown. We first reported of spontaneously immortalized cell line MIO-M1, but recently we derived similar lines from several eye donors. Since immortalization one main properties stem cells, investigated whether these...
Abstract Müller glia with stem cell characteristics have been identified in the adult human eye, and although there is no evidence that they regenerate retina vivo, can be induced to grow differentiate into retinal neurons vitro. We differentiated cells ganglion (RGC) precursors by stimulation fibroblast growth factor 2 together NOTCH inhibition using γ-secretase inhibitor N-[N-(3,5-difluorophenacetyl)-l-alanyl]-S-phenylglycine t-butyl ester (DAPT). Differentiation RGC was confirmed gene...
Abstract At present, there are severe limitations to the successful migration and integration of stem cells transplanted into degenerated retina restore visual function. This study investigated potential role chondroitin sulfate proteoglycans (CSPGs) microglia in human Müller glia with neural cell characteristics following subretinal injection Lister hooded (LH) Royal College Surgeons (RCS) rat retinae. Neonate LH showed minimal baseline microglial accumulation (CD68-positive cells) that...
Objective To evaluate the performance of a deep learning system (DLS) in classifying severity papilledema associated with increased intracranial pressure on standard retinal fundus photographs. Methods A DLS was trained to automatically classify 965 patients (2,103 mydriatic photographs), representing multiethnic cohort confirmed elevated pressure. Training performed 1,052 photographs mild/moderate (MP) and 1,051 severe (SP) classified by panel experts. The that 3 independent...
•The Brain and Optic Nerve Study Artificial Intelligence (BONSAI) deep learning system was able to reliably identify papilledema normal optic discs on photographs obtained in the Fundus photography vs Ophthalmoscopy Trial Outcomes Emergency Department (FOTO-ED) studies.•This previously trained high-quality mydriatic fundus performed very well nonmydriatic ocular photographs.•Our has excellent potential as a diagnostic aid emergency departments nonophthalmology clinics equipped with cameras....
Much controversy has arisen on the nature and sources of stem cells in adult human retina. Whilst ciliary epithelium been thought to constitute a source neural cells, population Müller glia retina also shown exhibit neurogenic characteristics. This study aimed compare proliferative abilities between these two major cell populations. It examined whether differences exist pigmented non-pigmented (CE) from eye. On this basis, with characteristics CE were isolated respectively. Expression glial,...
Purpose.: SOX2, a high-mobility group transcription factor, is expressed by retinal progenitors during development. It has been associated with the ability of progenitor cells to differentiate into neurons and highly human Müller stem (hMSCs) in culture. The authors investigated role this factor maintenance progenicity neural differentiation hMSCs vitro. Methods.: SOX2 silencing was induced transfection culture two pGSU6-GFP constructs scrambled control vector. Silencing confirmed...
Abstract Background Despite increasing demand, current protocols for human pluripotent stem cell (hPSC)-derived retinal pigment epithelium (RPE) remain time, labor, and cost intensive. Additionally, absence of robust methods selective RPE purification removal non-RPE impurities prevents upscaling clinical quality production. We aimed to address these challenges by developing a simplified hPSC-derived production system that yields high-quality monolayers within 90 days. Methods Human cells...
Purpose: Retinal pigment epithelial (RPE) cells grow well on surfaces that provide an extracellular matrix. Our aim was to establish primary adult human RPE cell cultures retain their morphology in vitro using amniotic membrane (hAM) as substrate. Materials and Methods: Human cadaver eyeballs (16) were obtained from the eye bank after corneal trephination. harvested by a) mechanical dissection of inner choroid surface (10, group 1) or b) enzymatic digestion 0.25% Trypsin/0.02% EDTA (6, 2)....
A 30-year-old woman presented with a palpable subcutaneous nodule in the areolar region of left breast. Sonomammographic examination revealed 2 cystic lesions showing typical "filarial dance" as vigorous twirling movement multiple curvilinear echoes mixed red blue color Doppler signals that was non-rhythmic, nonpulsatile, and characteristic pulse trace due to irregular worm movement. Real time sonographic demonstration these features is pathognomonic for filariasis, especially endemic areas...
Purpose: The Optic Neuritis Treatment Trial was a landmark study with implications worldwide. In the advent of antibody testing for neuromyelitis optica spectrum disease (NMOSD) and myelin oligodendrocyte glycoprotein antibody-associated (MOGAD), emerging concepts, such as routine management, remain controversial, resulting mostly from studies in White populations. We evaluate practice patterns optic neuritis investigation management by neuro-ophthalmologists neurologists Singapore. Design:...
Purpose: To investigate the relationship between lens vault (LV), visual acuity (VA), and refraction. Methods: This was a cross-sectional study of 2047 subjects aged 50 years older recruited from community polyclinic. Anterior segment optical coherence tomography performed, customized software used to measure LV. VA measured using logarithm minimum angle resolution chart (logMAR chart; Lighthouse Inc.), classified as normal (logMAR<0.3), mild impairment (0.3<logMAR<0.6), moderate/severe...
Abstract Introduction Australia has experienced sustained reductions in hepatitis C testing and treatment may miss its 2030 elimination targets. Addressing gaps community-based prescribing priority settings that did not have, or prioritise, care pathways is critical. Methods The Tasmanian Eliminate Hepatitis Outreach Project delivered a nurse-led outreach model of servicing populations the community through Statewide Sexual Health Service, supported by Eliminating partnership. Settings...
Completely isolated enteric duplication cysts are a rare variety of having an independent blood supply with no communication any part the adjacent bowel segment. We report case showing two completely originating in greater omentum and transverse mesocolon infant. Multiple involving non-contiguous segments have not been previously reported literature. In addition cyst was infected septations loss double wall sign resulting difficulty imaging diagnosis. Both were excised confirmed on histopathology.