A5024897573- Neuroscience and Neuropharmacology Research
- Photoreceptor and optogenetics research
- Retinal Development and Disorders
- Neural dynamics and brain function
- Glaucoma and retinal disorders
- Neuropeptides and Animal Physiology
- Memory and Neural Mechanisms
- Olfactory and Sensory Function Studies
- Receptor Mechanisms and Signaling
- Biochemical Analysis and Sensing Techniques
- Pain Mechanisms and Treatments
- Ion channel regulation and function
- Retinal Diseases and Treatments
- Neuroscience of respiration and sleep
- Alzheimer's disease research and treatments
- Visual perception and processing mechanisms
- Nicotinic Acetylcholine Receptors Study
- Adenosine and Purinergic Signaling
- Tryptophan and brain disorders
- Biochemical effects in animals
- Neurobiology and Insect Physiology Research
- Sleep and Wakefulness Research
- Neurological Disorders and Treatments
- Photochromic and Fluorescence Chemistry
- Neuroendocrine regulation and behavior
University College London
2012-2023
Institute of Ophthalmology
1988-2014
Moorfields Eye Hospital
2009-2012
Addex Therapeutics (Switzerland)
2012
Institut Paoli-Calmettes
2007
Imperial College London
2007
William Harvey Research Institute
2007
Inserm
2007
University of Geneva
2007
UCL Australia
2007
The development of the devastating neurodegenerative condition, Alzheimer's disease, is strongly associated with amyloid-beta (Abeta) deposition, neuronal apoptosis, and cell loss. Here, we provide evidence that implicates these same mechanisms in retinal disease glaucoma, a major cause irreversible blindness worldwide, previously simply effects intraocular pressure. We show Abeta colocalizes apoptotic ganglion cells (RGC) experimental glaucoma induces significant RGC apoptosis vivo dose-...
Age-related macular degeneration is the most common form of legal blindness in westernized societies, and polymorphisms gene encoding complement factor H (CFH) are associated with susceptibility to age-related more than half affected individuals. To investigate relationship between retinal disease, we performed functional anatomical analysis 2-year-old CFH-deficient (cfh(-/-)) mice. cfh(-/-) animals exhibited significantly reduced visual acuity rod response amplitudes on electroretinography...
The electrical activity of caudate neurones was recorded with intracellular electrodes in halothane anaesthetized cats. Agonists and antagonists excitatory amino acid receptors were applied by micro‐ionophoresis their effects on membrane‐ action potentials cortically evoked synaptic evaluated. agonists, L‐aspartate (asp), L‐glutamate (glu), N‐methyl‐DL‐aspartate (NMA), quinolinate quisqualate all depolarized the membrane, caused repetitive firing, reduced apparent amplitude post‐synaptic...
To assess the neuroprotective effects of different glutamate modulation strategies, with a nonselective (MK801) and selective (ifenprodil) NMDA receptor antagonist metabotropic agonist (mGluR Group II, LY354740), in glaucoma-related vivo rat models retinal ganglion cell (RGC) apoptosis.RGC apoptosis was induced Dark Agouti (DA) rats by staurosporine (SSP) treatment. Single agents MK801, ifenprodil, or LY354740, MK801 LY354740 combined, were administrated intravitreally at doses. Eyes imaged...
Abstract Müller glia with stem cell characteristics have been identified in the adult human eye, and although there is no evidence that they regenerate retina vivo, can be induced to grow differentiate into retinal neurons vitro. We differentiated cells ganglion (RGC) precursors by stimulation fibroblast growth factor 2 together NOTCH inhibition using γ-secretase inhibitor N-[N-(3,5-difluorophenacetyl)-l-alanyl]-S-phenylglycine t-butyl ester (DAPT). Differentiation RGC was confirmed gene...
Growing evidence points to a disruption of cortico-thalamo-cortical circuits in schizophrenia (SZ) and bipolar disorder (BD). Clues for specific involvement the thalamic reticular nucleus (TRN) come from its unique neuronal characteristics neural connectivity, allowing it shape thalamo-cortical information flow. A direct TRN SZ BD has not been tested thus far. We used combination human postmortem rodent studies test hypothesis that neurons expressing parvalbumin (PV neurons), main...
Nerve cell death is the key event in all neurodegenerative disorders, with apoptosis and necrosis being central to both acute chronic degenerative processes. However, until now, it has not been possible study these dynamically real time. In this study, we use spectrally distinct, well-recognised fluorescent markers enable temporal resolution quantification of early late phases single nerve cells different disease models. The tracking single-cell profiles same living eye over hours, days,...
1. Extracellular single‐neurone recordings were made in the ventrobasal thalamus (v.b.t.) of urethane‐anaesthetized rats with multi‐barrel ionophoretic electrodes order to test hypothesis that excitatory amino acid receptors are involved responses these neurones stimulation sensory afferents. 2. Responses either physiological hair and vibrissa follicle afferents ionophoretically applied acids challenged antagonists D‐2‐amino‐5‐phosphonovalerate (APV), kynurenate gamma‐D‐glutamylaminomethyl...
Metabotropic glutamate receptor 7 (mGlu<sub>7</sub>) has been suggested to be a promising novel target for treatment of range disorders, including anxiety, post-traumatic stress disorder, depression, drug abuse, and schizophrenia. Here we characterized potent selective mGlu<sub>7</sub> negative allosteric modulator (NAM) (+)-6-(2,4-dimethylphenyl)-2-ethyl-6,7-dihydrobenzo[<i>d</i>]oxazol-4(5<i>H</i>)-one (ADX71743). In vitro, Schild plot analysis reversibility tests at the confirmed NAM...
Progressive accumulation of age related mitochondrial DNA mutations reduce ATP production and increase reactive oxygen species output, leading to oxidative stress, inflammation degradation. The pace this is linked metabolic demand. retina has the greatest demand density in body displays progressive marked cell loss. Near infra-red (670 nm) thought be absorbed by cytochrome c oxidase (COX), a key element respiration it been demonstrated that improves membrane potentials aged eyes. It also...
Long-term depression (LTD) in the rodent superior colliculus (SC) is regarded as a model of synaptic refinement because it can be induced during development but not adults. We investigated role transient receptor potential vanilloid type-1 (TRPV1) channels this type plasticity. Experiments were carried out pigmented mice aged between postnatal day 8 (P8) and 42 (P42) adult mice. Retinal axons to SC labelled by injection cholera toxin-beta (CTbeta) into eye. Immunohistochemical staining for...