A5060629377- Glaucoma and retinal disorders
- Retinal Diseases and Treatments
- Retinal Development and Disorders
- Retinal Imaging and Analysis
- Retinal and Optic Conditions
- Corneal surgery and disorders
- Ocular Surface and Contact Lens
- Corneal Surgery and Treatments
- Artificial Intelligence in Healthcare and Education
- High Altitude and Hypoxia
- Parkinson's Disease Mechanisms and Treatments
- Alzheimer's disease research and treatments
- S100 Proteins and Annexins
- Telemedicine and Telehealth Implementation
- Coenzyme Q10 studies and effects
- Neuroscience and Neuropharmacology Research
- Flavonoids in Medical Research
- Cell Image Analysis Techniques
- Visual perception and processing mechanisms
- Retinopathy of Prematurity Studies
- Ophthalmology and Eye Disorders
- Nerve injury and regeneration
- Optical Imaging and Spectroscopy Techniques
- Nuclear Receptors and Signaling
- Neurological Disorders and Treatments
Imperial College London
2016-2025
Western Eye Hospital
2014-2024
Imperial College Healthcare NHS Trust
2014-2024
Western General Hospital
2013-2024
University of Oxford
2024
John Radcliffe Hospital
2024
University College London
2010-2019
National Health Service
2018
Moorfields Eye Hospital NHS Foundation Trust
2009-2015
Oxford Centre for Computational Neuroscience
2014
Dimethyl sulfoxide (DMSO) is an important aprotic solvent that can solubilize a wide variety of otherwise poorly soluble polar and nonpolar molecules. This, coupled with its apparent low toxicity at concentrations <10%, has led to ubiquitous use widespread application. Here, we demonstrate DMSO induces retinal apoptosis in vivo (5 μl intravitreally dosed rat from stock concentration 1, 2, 4, 8% v/v). Toxicity was confirmed vitro neuronal cell line, >1% (v/v), using annexin V, terminal...
Effective delivery to the retina is presently one of most challenging areas in drug development ophthalmology, due anatomical barriers preventing entry therapeutic substances. Intraocular injection only route administration for large protein therapeutics, including anti‐Vascular Endothelial Growth Factors Lucentis (ranibizumab) and Avastin (bevacizumab). Anti‐VEGFs have revolutionised management age‐related macular degeneration increasing indications use as sight‐saving therapies diabetes...
Neuropilin 1 (NRP1) is a transmembrane glycoprotein that essential for blood vessel development in vertebrates. Best known its ability to bind members of the vascular endothelial growth factor (VEGF) and class 3 semaphorin families through extracellular domain, it also has highly conserved cytoplasmic which terminates SEA motif binds PDZ protein synectin/GIPC1/NIP. Previous studies zebrafish embryos tissue culture models raised possibility NRP1 angiogenesis. Here, we describe generation mice...
See Herms and Schön (doi:10.1093/brain/awx100) for a scientific commentary on this article. Retinal cell apoptosis occurs in many ocular neurodegenerative conditions including glaucoma—the major cause of irreversible blindness worldwide. Using new imaging technique that we have called DARC (detection apoptosing retinal cells), which until now has only been demonstrated animal models, assessed if annexin 5 labelled with fluorescent dye DY-776 (ANX776) could be used safely humans to identify...
Parkinson's Disease (PD) is the second most common neurodegenerative disease worldwide, affecting 1 % of population over 65 years age. Dopaminergic cell death in substantia nigra and accumulation Lewy bodies are defining neuropathological hallmarks disease. Neuronal dysfunction have been reported other central nervous system regions, including retina. Symptoms PD typically manifest only when more than 70 dopaminergic cells lost, definitive diagnosis can be made histologically at post-mortem,...
Coenzyme Q10 (CoQ10) is a mitochondrial-targeted antioxidant with known neuroprotective activity. Its ocular effects when co-solubilised α–tocopherol polyethylene glycol succinate (TPGS) were evaluated. In vitro studies confirmed that CoQ10 was significantly protective in different retinal ganglion cell (RGC) models. vivo Adult Dark Agouti (DA) rats unilateral surgically-induced hypertension (OHT) treated either CoQ10/TPGS micelles or TPGS vehicle twice daily for three weeks performed,...
Nerve cell death is the key event in all neurodegenerative disorders, with apoptosis and necrosis being central to both acute chronic degenerative processes. However, until now, it has not been possible study these dynamically real time. In this study, we use spectrally distinct, well-recognised fluorescent markers enable temporal resolution quantification of early late phases single nerve cells different disease models. The tracking single-cell profiles same living eye over hours, days,...
Abstract The amyloid beta (A β ) pathway is strongly implicated in neurodegenerative conditions such as Alzheimer’s disease and more recently, glaucoma. Here, we identify the α 2 adrenergic receptor agonists ( 2ARA) used to lower intraocular pressure can prevent retinal ganglion cell (RGC) death via non-amyloidogenic A -pathway. Neuroprotective effects were confirmed vivo vitro different glaucoma-related models using 2ARAs brimonidine (BMD), clonidine (Clo) dexmedetomidine. 2ARA treatment...
Purpose to determine whether primary trabeculectomy or medical treatment produces better outcomes in term of quality life (QoL), clinical effectiveness and safety patients presenting with advanced glaucoma. Design multicentre randomised controlled trial Participants between June 3, 2014 May 31, 2017, 453 adults newly diagnosed open angle glaucoma at least one eye (Hodapp Classification) were recruited from 27 secondary care departments the UK, 227 allocated 226 management. Methods...
Involvement of the outer retina is controversial in glaucoma. The aim this study was to test, first, whether affected experimental ocular hypertension (OHT) and, second, whole retinal thickness can be used as a surrogate marker glaucomatous change.OHT surgically induced 20 Dark Agouti rats. Animals were imaged using modified Spectralis OCT (Heidelberg Engineering, Heidelberg, Germany) at baseline and 3 8 weeks after OHT induction. Measurements recorded for individual layer four...
A key objective in glaucoma is to identify those at risk of rapid progression and blindness. Recently, a novel first-in-man method for visualising apoptotic retinal cells called DARC (Detection-of-Apoptosing-Retinal-Cells) was reported. The aim develop an automatic CNN-aided spot detection enable prediction progression.
<h3>Aim</h3> To evaluate near-infrared (NIR) autofluorescence (AF) in patients with geographic atrophy (GA) secondary to age-related macular degeneration and investigate the origin of signal by vivo histological analysis rats a human donor eye. <h3>Methods</h3> Confocal scanning laser ophthalmoscopy imaging, including blue (excitation: 488 nm, emission 500–700 nm) NIR 790 >810 AF was performed 21 eyes 18 GA patients. Pigmented albino underwent same device both post-mortem imaging. For...
Abstract Cell-based therapies are increasingly recognized as a potential strategy to treat retinal neurodegenerative disease. Their administration, however, is normally indirect and complex, often with an inability assess in real time their effects on cell death migration/integration into the host retina. In present study, using partial optic nerve transection (pONT) rat model, we describe new method of Schwann (SC) delivery (direct application injured sheath, SC/DONS), which was compared...
Continuous-wave transscleral cyclophotocoagulation (CW-TSCP) is usually reserved for advanced/refractory glaucoma. Micropulse laser therapy (MPTLT) utilises short energy pulses separated by 'off'-periods. MPTLT postulated to have fewer complications, but its relative efficacy not known. The National Institute Health and Care Excellence (NICE) has deemed the evidence supporting use of inadequate quality, limiting research. This study aims evaluate safety compared CW-TSCP.
Secondary neurodegeneration is thought to play an important role in the pathology of neurodegenerative disease, which potential therapies may target. However, quantitative assessment degree secondary difficult. The present study describes a novel algorithm from estimates primary and degeneration are computed using well-established rodent models partial optic nerve transection (pONT) ocular hypertension (OHT). Brn3-labelled retinal ganglion cells (RGCs) were identified whole-retinal mounts...
Retinal ganglion cell (RGC) loss is one of the earliest and most important cellular changes in glaucoma. The DARC (Detection Apoptosing Cells) technology enables vivo real-time non-invasive imaging single apoptosing retinal cells animal models glaucoma Alzheimer’s disease. To date, RGCs imaged using have been counted manually. This time-consuming, labour-intensive, vulnerable to bias, has considerable inter- intra-operator variability. A semi-automated algorithm was developed which enabled...
Objective In a previous study, we reported the upregulation of Nerve Growth Factor (NGF) and trkANGFR expression in Ocular Cicatricial Pemphigoid (OCP), an inflammatory remodeling eye disease. Herein, hypothesize potential NGF-driven mechanism on fibroblasts (FBs) during OCP events. To verify, human derived OCP-FBs were isolated characterized either at baseline or after NGF exposure. Materials Methods Conjunctival biopsies obtained from 7 patients having 6 control subjects (cataract...