A5057550617- Intraocular Surgery and Lenses
- Retinal Development and Disorders
- Glaucoma and retinal disorders
- Retinal and Macular Surgery
- Corneal surgery and disorders
- Retinal Diseases and Treatments
- Virus-based gene therapy research
- Nuclear reactor physics and engineering
- Ocular Surface and Contact Lens
- Photoreceptor and optogenetics research
- Ophthalmology and Visual Impairment Studies
- RNA Interference and Gene Delivery
- Photochromic and Fluorescence Chemistry
- Traumatic Ocular and Foreign Body Injuries
- Retinoids in leukemia and cellular processes
- Ocular Infections and Treatments
- Nuclear Engineering Thermal-Hydraulics
- Ophthalmology and Eye Disorders
- Nuclear Physics and Applications
- Metallurgical Processes and Thermodynamics
- Corneal Surgery and Treatments
- Risk and Safety Analysis
- Retinopathy of Prematurity Studies
- Nuclear and radioactivity studies
- Nerve injury and regeneration
Osaka Hospital
2022-2025
Hyogo Prefectural Amagasaki General Medical Center
2008-2023
Osaka Red Cross Hospital
2014-2023
American Society of Colon and Rectal Surgeons
2018
American Society of Cataract and Refractive Surgery
2018
Kyoto Medical Center
2011-2012
Osaka University
2010
Kyoto University Hospital
2004-2008
Kyoto University
1993-2007
University of Michigan
2004-2007
The Maf-family transcription factor Nrl is a key regulator of photoreceptor differentiation in mammals. Ablation the gene mice leads to functional cones at expense rods. We show that 2.5-kb promoter segment directs expression enhanced GFP specifically rod photoreceptors and pineal gland transgenic mice. detected shortly after terminal cell division, corresponding timing genesis revealed by birthdating studies. In −/− retinas, GFP+ express S-opsin, consistent with transformation precursors...
Mutations in the retinitis pigmentosa GTPase regulator (RPGR) gene account for almost 20% of patients with pigmentosa. Most mutations are detected alternatively spliced RPGR-ORF15 isoform(s), which primarily but not exclusively expressed retina. We show that, addition to axoneme, protein is localized basal bodies photoreceptor connecting cilium and tip axoneme sperm flagella. Mass spectrometric analysis proteins that were immunoprecipitated from retinal axoneme-enriched fraction using an...
The optic nerve head in severely myopic eyes may be particularly vulnerable to glaucomatous damage. To study this hypothesis, we examined 122 primary open-angle glaucoma with fair good control of the intraocular pressure and a sign baseline Then, parameters for progression visual field defects were evaluated by multivariate analysis. A high mean (p = 0.007) large refractive error 0.023) significant risk factors subsequent loss. cup-to-disk ratio 0.100) was marginal factor. Nonsignificant...
Retrospective and prospective studies examined the surgical effects in lowering intraocular pressure levels of trabeculotomy ab externo adult eyes with either primary open angle glaucoma or pseudoexfoliation syndrome. We report results as an intervention for glaucoma.Included retrospective study were 357 227 patients 82 65 syndrome, each which underwent alone none had a history laser treatment ocular disease. Included 33 22 17 15 (older than 40 years) syndrome.A life-table analysis showed...
To systematically explore changes in gene expression the retina of monkeys with laser-induced glaucoma and to validate microarray data on eyes experimental glaucoma.Glaucoma was induced right eye four by repeated argon laser photocoagulation trabecular meshwork. The left served as control. Retinas were isolated from glaucomatous control 30 days after photocoagulation. Gene analyzed human chips which displayed a total 9182 elements including Expression Sequence Tag (EST) clones. Changes some...
To elucidate long-term surgical outcome of trabeculotomy ab externo in the treatment developmental glaucoma.Included this retrospective study are 116 eyes 71 patients with glaucoma. We classified into three groups based on their age: congenital (33 eyes), existing before age 2 months; infantile (31 occurring from ages months to years; and juvenile (52 years or older.A life-table analysis showed that total success probabilities at 5 10 one more operations were, respectively, 92.5% +/- 2.7%...
Although recent studies revealed chondroitinase ABC (ChABC), an enzyme that degrades chondroitin sulfate proteoglycans, promotes CNS regeneration in vivo, the usefulness of its application for transplantation is not clear. We investigated if treatment with ChABC can promote synapse formation between graft and host neurons following retinal transplantation. Dissociated cells were prepared from neonatal Nrl-GFP transgenic mice which rod photoreceptors their progenitor are labeled GFP. Each...
We invented a new method for fixing an intraocular lens (IOL) in the scleral tunnel without using wide conjunctival incision. Modified bent catheter needles were used to penetrate IOL haptics through sclerotomy sites. The inserted into 30-guage (G) tunnels guided by double 30-G piercing sclera. All procedures performed conjunctiva procedure does not require special forceps, trocars or fibrin glue, only and needles. aid of assistant was required support haptic. easily learnt based on our...
In nucleofractis during cataract surgery, inserting an ultrasound (US) tip near the posterior part of nucleus is difficult in limited space available. While disassembling harder/thicker nuclear cataracts, surgeons may damage zonule Zinn by pushing on with phacochopper and phaco-tip. this study, a chop technique described which inserted from US anterior part, sandwiching longitudinally to facilitate its crushing. The was successfully disassembled second third division. time mean cumulative...
NRL (neural retina leucine zipper) is a key basic motif-leucine zipper (bZIP) transcription factor, which orchestrates rod photoreceptor differentiation by activating the expression of rod-specific genes. The deletion Nrl in mice results functional cones that are derived from precursors. However, signaling pathways modulating or activity have not been elucidated. Here, we show retinoic acid (RA), diffusible factor implicated development, activates serum-deprived Y79 human retinoblastoma...
To assess the outcomes of 23-gauge sutureless transconjunctival vitrectomies (TSV), as compared with 25-gauge TSV in macular hole surgeries.A retrospective, consecutive, interventional case series 47 eyes idiopathic holes treated by 23- or were analysed.The operative time was 37.2 (SD 8.9) min and 34.2 (8.7) (p = 0.388). The anatomical success rate 96% 92% (p>0.999). logarithm minimum angle resolution best-corrected visual acuity (BCVA) at sixth postoperative month 0.19 (0.16) (0.25) 0.521)....
Many mammalian retinas are rod-dominant, and hence our knowledge of cone photoreceptor biology is relatively limited. To gain insights into the molecular differences between rods cones, we compared gene expression profile rod-dominated retina wild type mouse with that cone-only Nrl-/- (Neural leucine zipper knockout) mouse. Our analysis, using custom microarrays eye-expressed genes, provided equivalent data either direct or reference-based experimental designs, confirmed differential rod-...
Three patients with iridodialysis were recruited. A 6-0 polypropylene suture was cut to the proper length and flanged by cautery. long (19.0 mm), ultrathin 30-gauge needle inserted in anterior chamber from sclera around iris penetrated dialyzed iris, then exited through cornea on contralateral side. The unflanged After withdrawn, remained, passing sclera. ends of sutures until each flange reached It easy place multiple sutures. There no cases extrusion nor recurrence up 1 year. This...
The success of functional retinal cell transplantation has been limited by the low efficiency transplanted integration into host retina. Given that extracellular matrix (ECM) is thought to inhibit entry and axonal outgrowth grafted neural cells retina, modulation ECMs in environment may overcome this limitation. Here, we demonstrate metalloprotease-2 (MMP-2) expression associated with high migratory potential adult rat hippocampus-derived stem compared progenitor cells. In addition, MMP-2,...
We report a new intrascleral fixation technique for standard 3-piece posterior intraocular lens (IOL) using catheter needles and 30-gauge ultrathin instead of special forceps minimal manipulation in eyes without capsule support. Modified are used to deliver the IOL haptic. The haptic is secured extraocularly between cannula needle then advanced through sclerotomy site. Scleral tunnels created needles, which also serve as guide introduce haptics. This can be performed even cases miosis...
A female with von Hippel-Lindau (VHL) disease type 2A first presented erythrocytosis at the age of 9 years. This patient revealed multiple paragangliomas 22. After removal tumors, a retinal hemangioblastoma developed. Our diagnosis VHL was confirmed. Moreover, systemic examination showed duodenal somatostatinoma. Frequent and long-term monitoring is important for patients pheochromocytomas or paragangliomas, screening other hereditary cancer syndromes recommended. Recognition neuroendocrine...
To establish lines of transgenic mice that express Cre-recombinase in M- or S-cone photoreceptors for generating cone photoreceptor-specific (conditional) mutants.Five kilobases 5' upstream sequence the mouse red-green (M) opsin gene 0.5 kb blue (S) was cloned into a Cre-expression plasmid. Transgenic were generated and characterized, appropriate established. The Cre-transgenic crossed with ROSA26-lacZ (containing floxed beta-galactosidase gene) analyzed to determine...