A5081300110- Visual perception and processing mechanisms
- Neural dynamics and brain function
- Retinal Development and Disorders
- Neurobiology and Insect Physiology Research
- Retinal Diseases and Treatments
- Glaucoma and retinal disorders
- Memory and Neural Mechanisms
- Face Recognition and Perception
- Neuroscience and Neuropharmacology Research
- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- Cell Image Analysis Techniques
- Advanced Optical Imaging Technologies
- Pain Mechanisms and Treatments
- Medical Image Segmentation Techniques
- CCD and CMOS Imaging Sensors
- Botulinum Toxin and Related Neurological Disorders
- Photoreceptor and optogenetics research
- Photonic and Optical Devices
- Color perception and design
- Complementary and Alternative Medicine Studies
- Advanced Fluorescence Microscopy Techniques
- CRISPR and Genetic Engineering
- Retinopathy of Prematurity Studies
- Obsessive-Compulsive Spectrum Disorders
Center for Excellence in Brain Science and Intelligence Technology
2016-2024
Chinese Academy of Sciences
2015-2024
Shanghai Center for Brain Science and Brain-Inspired Technology
2020-2023
State Key Laboratory of Neuroscience
2019-2022
University College London
2000-2015
Shanghai Institutes for Biological Sciences
2015
Moorfields Eye Hospital
2012
National Institute for Health Research
2012
NIHR Biomedical Research Centre at The Royal Marsden and the ICR
2012
There is a tightly coupled bidirectional interaction between visual cortex and thalamus [lateral geniculate nucleus (LGN)]. Using drifting sinusoidal grating stimuli, we compared the response of cells in LGN with without feedback from cortex. Raster plots revealed striking difference pattern feedback. This was reflected results computing vector sum ratio zero harmonic to fundamental fast Fourier transform (FFT) for these responses. The variability responses assessed by using Fano factor also...
Many cells in both the central visual system and other sensory systems exhibit a center surround organization their receptive field, where response to centrally placed stimulus is modified when surrounding area also stimulated. This can follow from laterally directed connections local circuit at level of cell question but could involve more complex interactions. In lateral geniculate nucleus (LGN), relaying retinal input display concentric, that part follows similar characterizing providing...
The projections between the thalamus and primary visual cortex (V1) are a key reciprocal neural circuit, relaying retinal signals to cortical layers 4 & 6 while being simultaneously regulated by massive layer corticothalamic feedback. Effectively dissecting influence of this feedback circuit in higher mammals remains challenge for vision research. By pharmacologically increasing focal gain visually driven responses cat V1 controlled fashion, we examined effects such changes on response...
Feedback connections to early-level sensory neurons have been shown affect many characteristics of their neural response. Because selectivity for stimulus size is a fundamental property visual neurons, we examined the summation tuning and discretely mapped receptive field (RF) properties cells in lateral geniculate nucleus (LGN) both with without feedback from cortex. Using extracellular recording halothane-anesthetized cats, used small luminance probes displaced Cartesian coordinates...
Biological visual systems have evolved to process natural scenes. A full understanding of cortical functions requires a comprehensive characterization how neuronal populations in each area encode Here, we utilized widefield calcium imaging record V4 response tens thousands images male macaques. Using this large dataset, developed deep-learning digital twin that allowed us map the image preferences neural population at 100-µm scale. This detailed revealed diverse set functional domains V4,...
Significance Perceptually, the visual cortical areas are considered to reconstruct objects from diverse components of early distributed processing by grouping image elements and segregating them background as a figure. An assumption here is that raw, essentially unchanged information thalamus provides basic pattern essential for operation these higher-level abstractions. However, we demonstrate strong enhancement neuronal firing figure component figure-ground stimulus in recordings behaving...
Studying the mismatch between perception and reality helps us better understand constructive nature of visual brain. The Pinna–Brelstaff motion illusion is a compelling example illustrating how complex moving pattern can generate an illusory perception. When observer moves toward (expansion) or away (contraction) from figure, figure appears to rotate. neural mechanisms underlying complex-flow rotation, expansion, contraction remain unknown. We studied this question at both perceptual...
Primates need to detect and recognize camouflaged animals in natural environments. Camouflage-breaking movements are often the only visual cue available accomplish this. Specifically, sudden detected before full recognition of animal is made, suggesting that initial processing motion precedes motion-defined contours or shapes. What neuronal mechanisms underlying this primate brain? We investigated question using intrinsic-signal optical imaging macaque V1, V2 V4, along with computer...
Humans are more sensitive to luminance decrements than increments, as evidenced by lower thresholds and shorter latencies for dark stimuli. This asymmetry is consistent with results of neurophysiological recordings in dorsal lateral geniculate nucleus (dLGN) primary visual cortex (V1) cat monkey. Specifically, V1 population responses demonstrate that darks elicit higher levels activation brights, the latency OFF dLGN ON responses. The removal a or bright disc often generates perception...
The ventral visual pathway is crucially involved in integrating low-level features into complex representations for objects and scenes. At an intermediate stage of the pathway, V4 plays a crucial role supporting this transformation. Many neurons are selective shape segments like curves corners; however, it remains unclear whether these organized clustered functional domains, structural motif common across other cortices. Using two-photon calcium imaging awake macaques, we confirmed localized...
Visual fixation is a dynamic process, with the spontaneous occurrence of microsaccades and macrosaccades. These fixational saccades are sensitive to structural functional alterations cortical-subcortical-cerebellar circuit. Given that dysfunctional circuit contributes cognitive behavioral impairments in schizophrenia, we hypothesized patients schizophrenia would exhibit abnormal these abnormalities be associated clinical manifestations.
Rapid and efficient gene transduction via recombinant adeno-associated viruses (rAAVs) is highly desirable across many basic clinical research domains. Here, we report that vector co-infusion with doxorubicin, a anti-cancer drug, markedly enhanced rAAV-mediated transgene expression in the cerebral cortex mammalian species (cat, mouse, macaque), acting throughout time period examined detectable at just three days after transfection. This enhancement showed serotype generality, being common to...
Negative afterimages are perceptual phenomena that occur after physical stimuli disappear from sight. Their origin is linked to transient post-stimulus responses of visual neurons. The receptive fields (RFs) these subcortical ON- and OFF-center neurons exhibit antagonistic interactions between central surrounding space, resulting in selectivity for stimulus polarity size. These two features closely intertwined, yet their relationship negative afterimage perception remains unknown. Here we...
Metabotropic glutamate subtype 1 (mGlu1) receptor is thought to play a role in synaptic responses thalamic relay nuclei. The aim of this study was evaluate the positive allosteric modulator (PAM) Ro67-4853 as tool modulate mGlu1 receptors on single neurones vivo. Ro67-4853, applied by iontophoresis onto ventrobasal thalamus urethane-anaesthetised rats, selectively enhanced agonist (S)-3,5-dihydroxy-phenylglycine (DHPG), an effect consistent with potentiation. PAM also able enhance maintained...
Abstract The ventral visual pathway is crucially involved in integrating low-level features into complex representations for objects and scenes. At an intermediate stage of the pathway, V4 plays a crucial role supporting this transformation. Many neurons are selective shape segments like curves corners, however it remains unclear whether these organized clustered functional domains, structural motif common across other cortices. Using two-photon calcium imaging awake macaques, we confirmed...
Purpose: To physiologically examine the impairment of cortical sensitivity to visual motion during acute elevation intraocular pressure (IOP). Methods: Motion processing in cat brain is well characterized, its X and Y cell pathways being functionally analogous parvocellular magnocellular primates. Using this model, we performed ocular anterior chamber perfusion reversibly elevate IOP over a range from 30 90 mm Hg while monitoring activity with intrinsic signal optical imaging. Drifting...
Glaucoma is a leading cause of irreversible blindness worldwide, and intraocular pressure (IOP) an established modifiable risk factor for both chronic acute glaucoma. The relationship between color vision deficits glaucoma has been described previously. However, the effects or primary angle closure, which high prevalence in China, on remains unclear. To address above question, red-green blue-yellow responses V1, V2, V4 seven rhesus macaques were monitored using intrinsic-signal optical...