A5062003677- Retinal Development and Disorders
- Retinal Diseases and Treatments
- Retinal and Macular Surgery
- Glaucoma and retinal disorders
- Cell Adhesion Molecules Research
- Corneal Surgery and Treatments
- Pluripotent Stem Cells Research
- Photoreceptor and optogenetics research
- Intraocular Surgery and Lenses
- Neuroscience and Neural Engineering
- Neuroinflammation and Neurodegeneration Mechanisms
- Neuroscience and Neuropharmacology Research
- Nerve injury and regeneration
- Reproductive Biology and Fertility
- Protease and Inhibitor Mechanisms
- Photochromic and Fluorescence Chemistry
- Ocular Surface and Contact Lens
- Neurogenesis and neuroplasticity mechanisms
- RNA regulation and disease
- Advanced Glycation End Products research
- T-cell and B-cell Immunology
- Tissue Engineering and Regenerative Medicine
- Ocular Disorders and Treatments
- Monoclonal and Polyclonal Antibodies Research
- Ocular Diseases and Behçet’s Syndrome
University College London
2015-2024
Moorfields Eye Hospital NHS Foundation Trust
2011-2024
National Institute for Health Research
2014-2024
Moorfields Eye Hospital
2009-2024
University Hospitals of Morecambe Bay NHS Foundation Trust
2015
Institute of Ophthalmology
1999-2013
UCL Australia
2010
St Thomas' Hospital
1990-1999
King's College London
1999
Royal Victoria Hospital
1997
Abstract Growing evidence suggests that glial cells may have a role as neural precursors in the adult central nervous system. Although it has been shown Müller exhibit progenitor characteristics postnatal chick and rat retinae, their progenitor-like developed human retina is unknown. We first reported of spontaneously immortalized cell line MIO-M1, but recently we derived similar lines from several eye donors. Since immortalization one main properties stem cells, investigated whether these...
Abstract Müller glia with stem cell characteristics have been identified in the adult human eye, and although there is no evidence that they regenerate retina vivo, can be induced to grow differentiate into retinal neurons vitro. We differentiated cells ganglion (RGC) precursors by stimulation fibroblast growth factor 2 together NOTCH inhibition using γ-secretase inhibitor N-[N-(3,5-difluorophenacetyl)-l-alanyl]-S-phenylglycine t-butyl ester (DAPT). Differentiation RGC was confirmed gene...
Retinal gliosis is characterized by biochemical and physiological changes that often lead to Müller glia proliferation hypertrophy a feature of many neuro‐degenerative inflammatory diseases such as proliferative vitreoretinopathy (PVR). Although are known release factors cytokines, it not clear whether cytokine production these cells mirrors the pattern present in gliotic retina. Lysates from normal cadaveric retina retinal specimens patients undergoing retinectomy for treatment PVR, cell...
AIMS: This study investigated the presence of cytokine tumour necrosis factor alpha (TNF alpha) and vascular adhesion glycoproteins ICAM-1, VCAM-1, E-selectin, P-selectin, PECAM within fibrovascular membranes eyes with proliferative diabetic retinopathy (PDR). METHODS: The these molecules was determined by immunohistochemical staining using monoclonal antibodies APAAP technique. RESULTS: Staining for TNF observed on retinal endothelium five 12 specimens, infiltrating cells all membranes,...
The presence of interleukin 6 (IL-6), 1 (IL-1), 2 (IL-2) and tumour necrosis factor (TNF) was investigated in vitreous aqueous aspirates from eyes undergoing vitrectomy for the treatment different inflammatory conditions. Cadaveric 10 normal subjects were used as controls. IL-6 observed 5 specimens with idiopathic uveitis (range = 26–264 pg/ml), complicated retinal detachment (28 279 pg/ml, respectively), samples diabetic retinopathy 5–480 one sample an eye phacolytic glaucoma (1190 pg/ml)...
Abstract At present, there are severe limitations to the successful migration and integration of stem cells transplanted into degenerated retina restore visual function. This study investigated potential role chondroitin sulfate proteoglycans (CSPGs) microglia in human Müller glia with neural cell characteristics following subretinal injection Lister hooded (LH) Royal College Surgeons (RCS) rat retinae. Neonate LH showed minimal baseline microglial accumulation (CD68-positive cells) that...
Stem cell transplantation is a potential treatment strategy for neurodegenerative diseases such as glaucoma. The Müller stem line MIO-M1 can be differentiated to produce retinal neurons and glia. survival, migration, differentiation, integration of cells were investigated in rat model effect modulating the environment with either chondroitinase ABC or erythropoietin was also studied.Intraocular pressure chronically increased unilaterally by using laser glaucoma adult rats. EGFP-transduced...
Abstract Müller glia possess stem cell characteristics that have been recognized to be responsible for the regeneration of injured retina in fish and amphibians. Although these cells are present adult human eye, they not known regenerate vivo. Human with (hMSCs) can acquire phenotypic genotypic rod photoreceptors vitro, suggesting may potential use transplantation strategies treat photoreceptor degenerations. Much work has undertaken rodents using various sources allogeneic restore function,...
Abstract Human Müller glia with stem cell characteristics (hMGSCs) have been shown to improve retinal function upon transplantation into rat models of ganglion (RGC) depletion. However, their translational potential may depend successful engraftment and improvement in experimental anatomical functional features resembling those the human eye. We investigated effect allogeneic feline ability differentiate cells expressing RGC markers, following ablation RGCs by N-methyl-d-aspartate (NMDA)....
Glaucoma is one of the leading causes blindness, and there an ongoing need for new therapies. Recent studies indicate that cell transplantation using Müller glia may be beneficial, but a novel sources cells to provide therapeutic benefit. In this study, we have isolated from retinal organoids formed by human induced pluripotent stem (hiPSCs) in vitro shown their ability partially restore visual function rats depleted ganglion NMDA. Based on present results, suggest derived hiPSC attractive...