A5003372700- Reproductive System and Pregnancy
- Platelet Disorders and Treatments
- Blood groups and transfusion
- Hematopoietic Stem Cell Transplantation
- Reproductive Biology and Fertility
- Immune Cell Function and Interaction
- Brucella: diagnosis, epidemiology, treatment
- Cancer Cells and Metastasis
- Endometriosis Research and Treatment
- Mesenchymal stem cell research
- Immunodeficiency and Autoimmune Disorders
- Cancer, Hypoxia, and Metabolism
- Effects of Radiation Exposure
- Renal and related cancers
- Acute Myeloid Leukemia Research
- Immunotherapy and Immune Responses
- Chronic Lymphocytic Leukemia Research
- T-cell and B-cell Immunology
- Cancer Immunotherapy and Biomarkers
- Cytokine Signaling Pathways and Interactions
- Ovarian cancer diagnosis and treatment
- Virus-based gene therapy research
- CAR-T cell therapy research
- Ectopic Pregnancy Diagnosis and Management
- Salivary Gland Disorders and Functions
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2010-2024
Université Paris Cité
2005-2024
Hôpital Saint-Louis
2017-2024
Agence internationale de l'énergie
2021-2023
Sorbonne Paris Cité
2015-2023
Direction de la Recherche Fondamentale
2017-2021
University of London Institute in Paris
2020
Institut de recherche Saint-Louis
2020
Délégation Paris 7
2015-2017
Infectious Disease Models and Innovative Therapies
2010-2015
Immune checkpoints are powerful inhibitory molecules that promote tumor survival. Their blockade is now recognized as providing effective therapeutic benefit against cancer. Human leukocyte antigen G (HLA‐G), a recently identified immune checkpoint, has been detected in many types of primary tumors and metastases, malignant effusions well on tumor‐infiltrating cells, particularly patients with clear cell renal carcinoma (ccRCC). Here, order to define possible anticancer therapy, we used...
Despite some success, many patients do not benefit from immunotherapy. New strategies to improve clinical efficacy include identification of novel immune-checkpoint (IC) targets or a combination immunotherapy with antiangiogenic treatments. Here, we propose the therapeutic use IC, HLA-G/LILRB, and explore its enhanced synergistic antitumor activity when combined therapies.
The mechanisms that regulate the commitment of a totipotent stem cell to megakaryocytic lineage are largely unknown. Using molecular approach study megakaryocytopoiesis and platelet production, mice in which thrombocytopoiesis could be controlled were produced by targeting expression herpes simplex virus thymidine kinase toxigene megakaryocytes using regulatory region gene encoding alpha subunit integrin IIb beta 3. programmed eradication was induced treating transgenic bearing hybrid...
Ionizing radiation (IR) exposure causes rapid and acute bone marrow (BM) suppression that is reversible for nonlethal doses. Evidence accumulating IR can also provoke long-lasting residual hematopoietic injury. To better understand these effects, we analyzed phenotypic functional changes in the stem/progenitor compartment of irradiated mice over a 10-week period. We found stem cells (HSCs) identified by their repopulating ability continued to segregate within Hoechst dye excluding "side...
The establishment and maintenance of anti-tumor immune responses are the objectives cancer immunotherapy. Despite recent promising advances, effectiveness these approaches has been limited by multiple immunosuppressive mechanisms developed tumors (checkpoint). aim present study was to demonstrate intratumor heterogeneity at levels escape strategies tumor-host relationships. We focused on well-known checkpoints such as PD1/PDL1 a new checkpoint involving HLA-G its receptors ILT2/ILT4. A...
Mouse embryonic stem (ES) cells can be propagated in vitro while retaining their properties of pluripotency and self-renewal under the continuous presence leukemia inhibitor factor (LIF). An essential role has been attributed to subsequent activation Stat3 transcription mediating LIF response. To date, however, downstream target genes ES are still unknown. isolate these genes, we performed a microarray-based kinetic comparison LIF-stimulated (undifferentiated) versus induced differentiate by...
Abstract Background Clear cell renal carcinoma (ccRCC), the most aggressive cancer, is characterized by early lymph node metastases and bad prognosis. Most therapies targeting advanced or metastatic ccRCC are based, as first-line treatment, on administration of vascular endothelial growth factor (VEGF) neutralizing antibody termed Bevacizumab. Despite proven benefits, expected results were not obtained for majority patients. The possibility that an intricate interplay between angiogenesis...
Our understanding of system dynamics mixed cell populations in whole organisms has benefited from the advent individual marking by nonarrayed DNA barcodes subsequently analyzed high-throughput sequencing. However, key limitations include statistical biases compromising quantification and lack applicability to deconvolute fate vivo after pooling single cells differentially exposed different conditions ex vivo. Here, we have derived an arrayed lentiviral library obtained a proof-of-concept its...
Ionizing radiation causes rapid and acute suppression of hematopoietic cells that manifests as the syndrome. However, roles molecules regulatory pathways induced in vivo by irradiation different have not been completely elaborated. Using a strategy combined microarray bioinformatics tools, we identified gene networks might be involved early response vivo. The grouping similar time-ordered expression profiles using quality threshold clustering enabled successful identification common binding...
Cancer immunotherapies based mainly on the blockade of immune-checkpoint (IC) molecules by anti-IC antibodies offer new alternatives for treatment in oncological diseases. However, a considerable proportion patients remain unresponsive to them. Hence, development novel clinical immunotherapeutic approaches and/or targets are crucial.W In this context, targeting HLA-G/ILT2/ILT4 has caused great interest since it is abnormally expressed several malignancies generating tolerogenic...
Summary In microarray experiments, accurate estimation of the gene variance is a key step in identification differentially expressed genes. Variance models go from too stringent homoscedastic assumption to overparameterized model assuming specific for each gene. Between these two extremes there some room intermediate models. We propose method that identifies clusters genes with equal variance. use mixture on distribution. A test statistic ranking and detecting proposed. The illustrated...
With the use of Hoechst staining techniques, we have previously shown that C2C12 myogenic cell line contains a side population (SP) is largely increased in presence fibroblast growth factor 6 (FGF6). Here, compared transcriptional profiles from SP and main (MP) cells either or FGF6-expressing C2C12. Expression SPs show these are less differentiated than MPs display some similarities to stem cells. Moreover, principal component analysis made it possible distinguish specific contributions FGF6...
Purpose: To identify transcriptional gene-networks involved in the early vivo response of liver cells to radiation exposure and improve our understanding molecular processes responsible for tissue radiosensitivity.Materials methods: Transcriptome variations RNA samples were measured 3 hours post-irradiation using microarray technology. The results confirmed extended real-time polymerase-chain-reaction (RT-PCR).Results: We identified quantitative changes expression 126 genes, most which...
Understanding the role of Notch and its ligands within different bone marrow niches could shed light on mechanisms regulating haematopoietic progenitor cells (HPCs) maintenance self-renewal. Here, we report that murine HPCs activation by vascular Delta-4 ligand maintains a significant proportion specifically in G0 state. Furthermore, Delta-4/Notch pathway limits significantly loss vivo short-term reconstitutive potential upon transplantation activated into lethally irradiated recipient mice....