A5023183070- Cell Image Analysis Techniques
- Congenital heart defects research
- Virus-based gene therapy research
- CRISPR and Genetic Engineering
- Chromosomal and Genetic Variations
- Digital Imaging for Blood Diseases
- Reproductive System and Pregnancy
- CAR-T cell therapy research
- Image Processing Techniques and Applications
- Tissue Engineering and Regenerative Medicine
- Advanced biosensing and bioanalysis techniques
- Cancer-related molecular mechanisms research
- Plant Virus Research Studies
- 3D Printing in Biomedical Research
- RNA Research and Splicing
- Single-cell and spatial transcriptomics
- Pluripotent Stem Cells Research
Consejo Nacional de Investigaciones Científicas y Técnicas
2019-2024
Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia
2019-2021
Instituto de Neurología Cognitiva
2021
Deep learning is a significant step forward for developing autonomous tasks. One of its branches, computer vision, allows image recognition with high accuracy thanks to the use convolutional neural networks (CNNs). Our goal was train CNN transmitted light microscopy images distinguish pluripotent stem cells from early differentiating cells. We induced differentiation mouse embryonic epiblast-like and took at several time points initial stimulus. found that can be trained recognize...
PIWI-interacting RNAs (piRNAs) are a class of non-coding initially thought to be restricted exclusively germline cells. In recent years, accumulating evidence has demonstrated that piRNAs actually expressed in pluripotent, neural, cardiac and even cancer However, controversy remains around the existence function somatic piRNAs. Using small RNA-seq samples from H9 pluripotent cells differentiated mesoderm progenitors cardiomyocytes we identified expression 447 piRNA transcripts, which 241...
Cell death experiments are routinely done in many labs around the world, these backbone of assays for drug development. detection is usually performed ways, and requires time reagents. However, cell preceded by slight morphological changes shape texture. In this paper, we trained a neural network to classify cells undergoing death. We found that was able highly predict after one hour exposure camptothecin. Moreover, prediction largely outperforms human ability. Finally, provide simple python...
Abstract The stem cell niche has a strong influence in the differentiation potential of human pluripotent cells with integrins playing major role communicating extracellular environment. However, it is not well understood how interactions between and matrix are involved cardiac differentiation. To evaluate this, we performed profile expression two stages differentiation: mesodermal progenitors cardiomyocytes. We found an active regulation different during In particular, integrin α 5 subunit...
Cancer immunotherapies based mainly on the blockade of immune-checkpoint (IC) molecules by anti-IC antibodies offer new alternatives for treatment in oncological diseases. However, a considerable proportion patients remain unresponsive to them. Hence, development novel clinical immunotherapeutic approaches and/or targets are crucial.W In this context, targeting HLA-G/ILT2/ILT4 has caused great interest since it is abnormally expressed several malignancies generating tolerogenic...
Abstract Cardiac development is a finely regulated process, transforming undifferentiated cells into the specialized cell components of heart. Super-Enhancers (SE), clusters enhancers densely populated with transcription factors, play central role in fate decisions, including cardiogenesis. In this work, we studied cardiac SE chr3q25.31. This locus also contains specific long non-coding RNA: LINC00881 (LINC881). We termed region interest SHEN-LONG ( S uper H eart EN hancer and LONG LINC881),...
Abstract Cell death experiments are routinely done in many labs around the world, these backbone of assays for drug development. detection is usually performed ways, and requires time reagents. However, cell preceded by slight morphological changes shape texture. In this paper, we trained a neural network to classify cells undergoing death. We found that was able highly predict after one hour exposure camptothecin. Moreover, prediction largely outperforms human ability. Finally, provide...
Summary PIWI-interacting RNAs (piRNAs) are a class of non-coding initially thought to be restricted almost exclusively germ line cells. In recent years, accumulating evidence has demonstrated that piRNAs actually expressed in somatic cells like pluripotent, neural, cardiac and even cancer However, controversy still remains around the existence function piRNAs. Using small RNA-seq samples from H9 pluripotent stem differentiated mesoderm progenitors cardiomyocytes we identified expression 447...
Abstract Cancer immunotherapies based mainly on the blockade of immune-checkpoint (IC) molecules by anti-IC antibodies offer new alternatives for treatment in oncological diseases. However, a considerable proportion patients remain unresponsive to them. Hence, development novel clinical immunotherapeutic approaches and/or targets are crucial. In this context, targeting HLA-G/ILT2/ILT4 has caused great interest since it is abnormally expressed several malignancies generating tolerogenic...