A5079376137- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Tissue Engineering and Regenerative Medicine
- RNA Research and Splicing
- Mesenchymal stem cell research
- Advanced biosensing and bioanalysis techniques
- Chromosomal and Genetic Variations
- MicroRNA in disease regulation
- Electrospun Nanofibers in Biomedical Applications
- Cytokine Signaling Pathways and Interactions
- Genomics and Chromatin Dynamics
- Molecular Biology Techniques and Applications
- Congenital heart defects research
- Advanced Fluorescence Microscopy Techniques
- Plant Virus Research Studies
- Viral Infections and Immunology Research
- PI3K/AKT/mTOR signaling in cancer
- Circular RNAs in diseases
- Cardiac Ischemia and Reperfusion
Centre for Genomic Regulation
2022-2024
Consejo Nacional de Investigaciones Científicas y Técnicas
2015-2020
Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia
2015-2019
Abstract Human embryonic and induced pluripotent stem cells are self-renewing (PSC) that can differentiate into a wide range of specialized cells. Basic fibroblast growth factor is essential for PSC survival, stemness self-renewal. PI3K/AKT pathway regulates cell viability apoptosis in many types. Although it has been demonstrated activation by bFGF relevant maintenance its role on survival remains elusive. In this study we explored the molecular mechanisms involved regulation AKT. We found...
Mesenchymal stem cells (MSCs) are a promising source of for regenerative therapies. Although they can be isolated easily from several tissues, cell expansion is limited since their properties lost with successive passages. Hence, pluripotent derived MSCs (PD-MSCs) arise as suitable alternative MSC production. Nevertheless, at present, PD-MSC derivation protocols either expensive or not clinical purposes.In this work we present therapy-grade, inexpensive and simple protocol to derive (PSCs)...
MicroRNAs are small non-coding RNAs involved in post-transcriptional regulation of gene expression related to many cellular functions. We performed a small-RNAseq analysis cardiac differentiation from pluripotent stem cells. Our analyses identified some new aspects about microRNA this process. First, we described dynamic profile microRNAs where them clustered according their level. Second, the extensive network isomiRs and ADAR modifications. Third, families clusters establishment lineage...
PIWI-interacting RNAs (piRNAs) are a class of non-coding initially thought to be restricted exclusively germline cells. In recent years, accumulating evidence has demonstrated that piRNAs actually expressed in pluripotent, neural, cardiac and even cancer However, controversy remains around the existence function somatic piRNAs. Using small RNA-seq samples from H9 pluripotent cells differentiated mesoderm progenitors cardiomyocytes we identified expression 447 piRNA transcripts, which 241...
Abstract The stem cell niche has a strong influence in the differentiation potential of human pluripotent cells with integrins playing major role communicating extracellular environment. However, it is not well understood how interactions between and matrix are involved cardiac differentiation. To evaluate this, we performed profile expression two stages differentiation: mesodermal progenitors cardiomyocytes. We found an active regulation different during In particular, integrin α 5 subunit...
Abstract During early development, gene expression is tightly regulated. However, how genome organization controls during the transition from naïve embryonic stem cells to epiblast still poorly understood. Using single-molecule microscopy approaches reach nanoscale resolution, we show that remodeling affects transcription pluripotency transition. Specifically, after exit state, chromatin becomes less compacted, and OCT4 factor has lower mobility more bound its cognate sites. In cells, active...
Cell reprogramming has been well described in mouse and human cells. The expression of specific microRNAs demonstrated to be essential for pluripotent maintenance cell differentiation, but not much information is available domestic species. We aim generate horse iPSCs, characterize them evaluate the different (miR-302a,b,c,d, miR-205, miR-145, miR-9, miR-96, miR-125b miR-296). Two equine iPSC lines (L2 L3) were characterized after fibroblasts with four Yamanaka's factors...
Summary PIWI-interacting RNAs (piRNAs) are a class of non-coding initially thought to be restricted almost exclusively germ line cells. In recent years, accumulating evidence has demonstrated that piRNAs actually expressed in somatic cells like pluripotent, neural, cardiac and even cancer However, controversy still remains around the existence function piRNAs. Using small RNA-seq samples from H9 pluripotent stem differentiated mesoderm progenitors cardiomyocytes we identified expression 447...