A5076063501- Glycosylation and Glycoproteins Research
- Proteoglycans and glycosaminoglycans research
- Mass Spectrometry Techniques and Applications
- Seaweed-derived Bioactive Compounds
- Microfluidic and Capillary Electrophoresis Applications
- Carbohydrate Chemistry and Synthesis
- Nanopore and Nanochannel Transport Studies
- Metabolomics and Mass Spectrometry Studies
- Marine Sponges and Natural Products
- Enzyme Structure and Function
- Analytical Chemistry and Chromatography
- Complement system in diseases
- Advanced Biosensing Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Polysaccharides Composition and Applications
- Electrochemical sensors and biosensors
- Microbial Metabolites in Food Biotechnology
- Polyamine Metabolism and Applications
- Ion-surface interactions and analysis
- Biochemical effects in animals
- Hemoglobin structure and function
- Marine and coastal plant biology
- Monoclonal and Polyclonal Antibodies Research
- Amino Acid Enzymes and Metabolism
- Microfluidic and Bio-sensing Technologies
Université d'Évry Val-d'Essonne
2010-2023
Laboratoire Analyse et Modélisation pour la Biologie et l'Environnement
2014-2023
Centre National de la Recherche Scientifique
2013-2023
Université Paris-Saclay
2017-2023
CY Cergy Paris Université
2020-2021
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2005-2018
CEA Paris-Saclay
2017-2018
Chimie ParisTech
1994-2007
Sorbonne Université
1998-2007
Institut de Biologie Structurale
2005
The implementation of a reliable, rapid, inexpensive, and simple method for whole-proteome identification would greatly benefit cell biology research clinical medicine. Proteins are currently identified by cleaving them with proteases, detecting the polypeptide fragments mass spectrometry, mapping latter to sequences in genomic/proteomic databases. Here, we demonstrate that can instead be detected classified at single-molecule limit using nanometer-scale pore formed protein aerolysin....
Glycosaminoglycans are biologically active anionic carbohydrates that among the most challenging biopolymers with regards to their structural analysis and functional assessment. The potential of newly introduced biosensors using protein nanopores have been mainly described for nucleic acids date, has here applied this polysaccharide-based third class bioactive biopolymer. This nanopore approach harnessed in study analyze hyaluronic acid glycosamiglycan its depolymerization-derived...
Abstract Glycosaminoglycans are highly anionic functional polysaccharides with information content in their structure that plays a major role the communication between cell and extracellular environment. The study presented here reports label-free detection analysis of glycosaminoglycan molecules at single molecule level using sensing by biological nanopore, thus addressing need to decipher structural oligo- polysaccharide sequences, which remains challenge for glycoscience. We demonstrate...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPreparation and Evaluation of Immunosorbents for Selective Trace Enrichment Phenylurea Triazine Herbicides in Environmental WatersV. Pichon, L. Chen, M.-C. Hennion, R. Daniel, A. Martel, F. Le Goffic, J. Abian, D. BarceloCite this: Anal. Chem. 1995, 67, 14, 2451–2460Publication Date (Print):July 15, 1995Publication History Published online1 May 2002Published inissue 15 July...
Complement C1q is a hexameric molecule assembled from 18 polypeptide chains of three different types encoded by genes. This versatile recognition protein senses wide variety immune and nonimmune ligands, including pathogens altered self components, triggers the classical complement pathway through activation its associated proteases C1r C1s. We report method for expression recombinant full-length human involving stable transfection HEK 293-F mammalian cells fusion an affinity tag to...
Sulfs are extracellular sulfatases that have emerged recently as critical regulators of heparan sulfate (HS) activities through their ability to catalyze specific 6-O-desulfation the polysaccharide. Consequently, been involved in many physiological and pathological processes, notably for Sulf-2, development cancers with poor prognosis. Despite growing interest, little is known about structure activity these enzymes way they induce dynamic remodeling HS 6-O-sulfation status. Here, we combined...
IR spectroscopy of gas-phase ions is proposed to resolve positional isomers sulfated carbohydrates. Mass spectrometric fingerprints and vibrational spectra in the near mid-IR regions were obtained for monosaccharides, yielding unambiguous signatures isomers. We report first systematic exploration biologically relevant but notoriously challenging deprotonated state region. Remarkably, anions displayed very atypical profiles, which challenge well-established DFT (Density Functionnal Theory)...
Intravascular erythrocyte destruction, accompanied by the release of pro-oxidative and pro-inflammatory components hemoglobin heme, is a common event in pathogenesis numerous diseases with heterogeneous etiology clinical features. A frequent adverse effect related to massive hemolysis renal injury inflammation. Nevertheless, it still unclear whether heme – danger-associated molecular pattern ligand for TLR4 or upstream hemolysis-derived products are responsible these effects....
Algal fucoidan is an α-l-fucose-based polysaccharide endowed with important biological properties for which the structure has not yet been fully elucidated. In attempt to implement new enzymatic tools structural study of this polysaccharide, we have found a fucosidase activity in digestive glands common marine mollusk Pectenmaximus, active on extracted from brown algae Ascophyllum nodosum. We now report purification and characterization α-l-fucosidase (EC 3.2.1.51). The enzyme was purified...
Fucoidan is a potent inhibitor of the human complement system whose activity mediated through interactions with certain proteins belonging to classical pathway, particularly protein C4. Branched fucoidan oligosaccharides displayed higher anticomplementary as compared linear structures. Nuclear magnetic resonance (NMR) characterization branched and saturation transfer difference-NMR experiment interaction C4 allowed identification glycan residues in close contact target protein. Transferred...
Compared to other analytical methods, matrix‐assisted laser desorption ionization mass spectrometry (MALDI‐MS) presents several unique advantages for the structural characterization of degradation products carbohydrates. Our final goal is implement this technique as a high‐throughput platform, with aim exploring natural bio‐diversity discover new carbohydrate depolymerizing enzymes. In approach, variety carbohydrates will be used enzymes substrates and MALDI‐MS employed monitor...
Most of the recent developments aiming to coupling between surface plasmon resonance (SPR) and mass spectrometry (MS) are based on use a biochip with limited number flow cells requiring elution steps for recovery captured biomolecules. In this work, direct on-chip MALDI-MS detection is presented using SPRi-sensor in microarray format that allows multiplex SPR−MS analysis. The gold was functionalized by self-assembled monolayer (SAM) short polyoxyethylene (POE) chains carrying...
Sulfs represent a class of unconventional sulfatases which provide an original post-synthetic regulatory mechanism for heparan sulfate polysaccharides and are involved in multiple physiopathological processes, including cancer. However, remain poorly characterized enzymes, with major discrepancies regarding their vivo functions. Here we show that human Sulf-2 (HSulf-2) harbors chondroitin/dermatan glycosaminoglycan (GAG) chain, attached to the enzyme substrate-binding domain. We demonstrate...
Alpha-synuclein is one of several key factors in the regulation nerve activity. It striking that single- or multiple-point mutations 140-amino-acid-long protein can change its structure, which leads to protein's aggregation and fibril formation (which associated with neurodegenerative diseases, e.g., Parkinson's disease). We recently demonstrated a single nanometer-scale pore identify proteins based on ability discriminate between protease-generated polypeptide fragments. show here variation...
Chemokine stromal cell-derived factor-1 (SDF-1) is a potent chemoattractant involved in leukocyte trafficking and metastasis. Heparan sulfate on the cell surface binds SDF-1 may modulate its function as coreceptor of this chemokine. A major effect glycosaminoglycan binding be quaternary structure SDF-1, which has been controversially reported monomer or dimer. We have investigated sulfated oligosaccharides oligomerization mutated form (3/6), using affinity capillary electrophoresis (ACE)...
Polysulfated carbohydrates such as heparin (HP) and heparan sulfate (HS) are not easily amenable to usual ultraviolet matrix-assisted laser desorption/ionization-mass spectrometry (UV-MALDI)-MS analysis due the thermal lability of their O- N-SO3 moieties, poor ionization efficiency with common crystalline matrices. Recently, ionic liquid matrices showed considerable advantages over conventional for MALDI-MS acidic compounds. Two new (ILMs) based on combination 2-(4-hydroxyphenylazo)benzoic...
Abstract— —High circulating levels of l ‐methionine produced by inclusion in the diet or parenteral injection amino acid caused alterations free pattern liver and brain tissues. Acute effects following were more pronounced than those long term feeding where adaptation played a role. The net effect was to increase total acids while decreasing brain. Individually, hepatic aspartic acid, threonine, serine, glutamine, glutamic glycine, alanine depressed taurine, cystathionine, methionine,...