A5063361434- Forensic Toxicology and Drug Analysis
- Drug Transport and Resistance Mechanisms
- Pharmacological Effects and Toxicity Studies
- Neurotransmitter Receptor Influence on Behavior
- Pituitary Gland Disorders and Treatments
- Cannabis and Cannabinoid Research
- Hormonal Regulation and Hypertension
- Adrenal and Paraganglionic Tumors
- Adrenal Hormones and Disorders
- Tea Polyphenols and Effects
- Psychedelics and Drug Studies
- Analytical Chemistry and Chromatography
- Sirtuins and Resveratrol in Medicine
- Mass Spectrometry Techniques and Applications
- Adipose Tissue and Metabolism
- Glioma Diagnosis and Treatment
- Cancer, Hypoxia, and Metabolism
- Trace Elements in Health
- Thermal and Kinetic Analysis
- Phytochemicals and Antioxidant Activities
- Hormonal and reproductive studies
- Medical Device Sterilization and Disinfection
- Ovarian function and disorders
- Metabolomics and Mass Spectrometry Studies
- Reproductive Biology and Fertility
Institut de Chimie
2021-2024
Laboratoire de Chimie Physique
2022-2024
Université Paris-Saclay
2021-2024
Centre National de la Recherche Scientifique
2022-2024
Université Paris Cité
2012-2023
Optimisation Thérapeutique en Neuropsychopharmacologie
2019-2023
Délégation Paris 5
2008-2020
Inserm
2015-2020
Délégation Paris 7
2015-2020
Hôpital Cochin
2016-2020
Black tea contains two major groups of pigments, theaflavins (TFs) and thearubigins (TRs). TFs contain a bis-flavan substituted 1,2-dihydroxy-3,4-benzotropolone moiety. Unlike the TFs, TRs have not yet been characterized. The chemical structure remains mystery. present paper reports our effort to study using delayed pulsed ion extraction ions generated via matrix-assisted laser desorption ionization (MALDI) technique, on line with Linear time-of-flight (TOF) mass spectrometer. Spectra...
The effect of cannabidiol (CBD), a high-affinity agonist the transient receptor potential vanilloid-2 (TRPV2) channel, has been poorly investigated in human brain microvessel endothelial cells (BMEC) forming blood–brain barrier (BBB). TRPV2 expression and its role on Ca2+ cellular dynamics, trans-endothelial electrical resistance (TEER), cell viability growth, migration, tubulogenesis were evaluated primary cultures BMEC (hPBMEC) or cerebral hCMEC/D3 line. Abundant was measured hPBMEC by...
Abstract Endothelial cells (ECs) are constantly submitted in vivo to hemodynamical forces derived from the blood circulation, including shear stress (SS). ECs able detect SS and consequently adapt their phenotype, thus affecting many endothelial functions. If a plethora of stress-regulated molecular networks have been described peripheral ECs, less is known about responses microvascular brain which constitute blood–brain barrier (BBB). In this work, we investigated response human cerebral...
Trans-resveratrol is widely studied for its potentially beneficial effects on numerous disorders. It rapidly metabolized and metabolites can exhibit biological activity. The present study aimed to investigate whether acute or sustained trans-resveratrol administration impacted the distribution of in brain, heart, liver. We used ultra-HPLC quadrupole-TOF (UHPLC-Q-TOF) a full-scan mode identify assess large numbers resveratrol metabolites. For intake, mice were overfed with single dose (150...
Organic cation transporters (OCTs) participate in the handling of compounds kidneys and at synaptic cleft. Their role blood-brain barrier (BBB) brain drug delivery is still unclear. The presence OCT1,2,3 (SLC22A1-3) mouse, rat human isolated microvessels was investigated by either qRT-PCR, quantitative proteomics and/or functional studies. BBB transport prototypical substrate [3H]-1-methyl-4-phenylpyridinium ([3H]-MPP+) measured situ perfusion six mouse strains Sprague Dawley rats, primary...
Breast cancer resistance protein (BCRP) is expressed in various tissues, such as the gut, liver, kidney and blood brain barrier (BBB), where it mediates unidirectional transport of substrates to apical/luminal side polarized cells. Thereby BCRP acts an efflux pump, mediating elimination or restricting entry endogenous compounds xenobiotics into tissues plays important roles drug disposition, efficacy safety. Bcrp knockout mice (Bcrp<sup>−/−</sup>) have been used widely study role this...
The blood-brain barrier (BBB) hinders the brain delivery of many anticancer drugs. In pediatric patients, diffuse intrinsic pontine glioma (DIPG) represents main cause cancer mortality lacking effective drug therapy. Using sham and DIPG-bearing rats, we analyzed 1) distribution 3-kDa-Texas red-dextran (TRD) or [14C]-sucrose as measures BBB integrity, 2) role major ATP-binding cassette (ABC) transporters at on efflux irinotecan metabolite [3H]-SN-38. unaffected TRD in cerebrum, cerebellum,...
The blood-brain barrier (BBB) is pivotal for the maintenance of brain homeostasis and it strictly regulates cerebral transport a wide range endogenous compounds drugs. While fasting increasingly recognized as potential therapeutic intervention in neurology psychiatry, its impact upon BBB has not been studied. This study was designed to assess global repertoire transporters. We used combination vivo vitro experiments response endothelium male rats that were fed ad libitum or fasted one three...
A heterogeneous system between vitamin C and stearic acid was characterized by thermal crystallographic analyses. The results showed that such a prevents from decomposition. evidence provided implementing new protocol associating chromatic assays analyses in order to quantify the percentage of non-degraded C. collected with C–stearic mixtures allowed deducing coherent interpretation obtained pure at different heating scan rates. Vitamin mainly degrades upon melting but also solid state for...
Liquid chromatography coupled to tandem mass spectrometry-based targeted absolute protein quantification (in fmol of the analyte per μg total protein) is employed for molecular characterization blood-brain barrier using isolated brain microvessels. Nevertheless, heterogeneity sample regarding levels different cells co-isolated within microvessels and bovine serum albumin (BSA) contamination (from buffers) are not always evaluated. We developed an unlabeled liquid spectrometry method survey...
The grey mouse lemur (Microcebus murinus) is a non-human primate used to study the ageing process. Resveratrol polyphenol that may increase lifespan by delaying age-associated pathologies. However, no information about resveratrol absorption and metabolism available for this primate. its metabolites were qualitatively quantitatively analyzed in male mouse-lemur plasma (after 200 mg.kg−1 of oral resveratrol) ultra-high performance liquid chromatography (UHPLC), coupled...
The exact solid–liquid equilibrium between ascorbic acid and acetaminophen was established combining high performance liquid chromatography differential scanning calorimetry.
The pharmacokinetics of the recreational drug 3,4-methylenedioxymethamphetamine (MDMA) and its mains metabolites have never been modeled together. We therefore designed a model with which to analyze MDMA, 3,4-methylenedioxyamphetamine (MDA), 4-hydroxy-3-methoxymethamphetamine (HMMA), 4-hydroxy-3-methoxyamphetamine (HMA) test effect covariates like gender body weight on pharmacokinetics. Rats (18 males 18 females) were given 1 mg/kg MDMA iv, concentrations MDA, HMMA measured by...