A5027051818- Plant Virus Research Studies
- CRISPR and Genetic Engineering
- Biochemical and Molecular Research
- Mosquito-borne diseases and control
- Advanced biosensing and bioanalysis techniques
- RNA modifications and cancer
- Cancer Research and Treatments
- 3D Printing in Biomedical Research
- Viral Infections and Vectors
- Bacteriophages and microbial interactions
- Viral Infections and Immunology Research
- Viral Infections and Outbreaks Research
- SARS-CoV-2 and COVID-19 Research
Centre National de la Recherche Scientifique
2019-2024
Aix-Marseille Université
2019-2024
Architecture et Fonction des Macromolécules Biologiques
2019-2023
Alphaviruses, such as chikungunya virus (CHIKV), are mosquito-borne viruses that represent a significant threat to human health due the current context of global warming. Efficient alphavirus infection relies on activity non-structural protein 3 (nsP3), puzzling multifunctional molecule whose role in remains largely unknown. NsP3 is component plasma membrane-bound viral RNA replication complex (vRC) essential for amplification and also found large cytoplasmic aggregates unknown function
Bemnifosbuvir (AT-527) and AT-752 are guanosine analogues currently in clinical trials against several RNA viruses. Here, we show that these drugs require a minimal set of 5 cellular enzymes for activation to their common 5′-triphosphate AT-9010, with an obligate order reactions. AT-9010 selectively inhibits essential viral enzymes, accounting antiviral potency. Functional structural data at atomic resolution decipher N 6 -purine deamination compatible its metabolic activation. Crystal...
Bemnifosbuvir (AT-527) and AT-752 are guanosine analogues currently in clinical trials against several RNA viruses. Here we show that these drugs require a minimal set of 5 cellular enzymes for activation to their common 5'-triphosphate AT-9010, with an obligate order reactions. AT-9010 selectively inhibits essential viral enzymes, accounting broad spectrum antiviral potency. Functional structural data at atomic resolution decipher N6-purine deamination compatible metabolic by human ADALP1....
In the arms race against bacteria, bacteriophages have evolved diverse anti-CRISPR proteins (Acrs) that block CRISPR-Cas immunity. Acrs play key roles in molecular coevolution of bacteria with their predators, use a variety mechanisms action, and provide tools to regulate Cas-based genome manipulation. Here, we present structural functional analyses AcrIIA6, an Acr from virulent phages, exploring its unique action. Our cryo-EM structures data AcrIIA6 binding Streptococcus thermophilus Cas9...