A5034258047- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Advanced Biosensing Techniques and Applications
- Single-cell and spatial transcriptomics
- COVID-19 Clinical Research Studies
- Immune cells in cancer
- Aquaculture disease management and microbiota
- SARS-CoV-2 and COVID-19 Research
- Monoclonal and Polyclonal Antibodies Research
- Cancer Cells and Metastasis
- RNA modifications and cancer
- SARS-CoV-2 detection and testing
- Sperm and Testicular Function
- Helicobacter pylori-related gastroenterology studies
- Nanoplatforms for cancer theranostics
- COVID-19 and Mental Health
- Epigenetics and DNA Methylation
- Aquaculture Nutrition and Growth
- Cancer Research and Treatments
- Long-Term Effects of COVID-19
- Esophageal Cancer Research and Treatment
- Immune Cell Function and Interaction
- Immune Response and Inflammation
- Cancer Genomics and Diagnostics
Fundación Arturo López Pérez
2019-2025
Austral University of Chile
2009-2025
San Sebastián University
2024-2025
Advanced Center for Chronic Diseases
2025
Yale University
2017-2022
Universidad de Santiago de Chile
2021
University of New Haven
2017-2020
Aristotle University of Thessaloniki
2018
Fluidigm (United States)
2018
Rensselaer Polytechnic Institute
2018
Purpose: Determine the localized expression pattern and clinical significance of VISTA/PD-1H in human non-small cell lung cancer (NSCLC).Experimental Design: Using multiplex quantitative immunofluorescence (QIF), we performed measurements VISTA, PD-1, PD-L1 protein 758 stage I-IV NSCLCs from 3 independent cohorts represented tissue microarray format. The targets were selectively measured cytokeratin+ tumor epithelial cells, CD3+ T CD4+ T-helper CD8+ cytotoxic CD20+ B lymphocytes CD68+...
Abstract Specific mechanisms by which tumor-infiltrating lymphocytes (TIL) become dysfunctional remain poorly understood. Here, we employed a two-pronged approach using single-cell mass cytometry and tissue imaging technologies to dissect TILs from 25 patients with resectable 35 advanced non–small cell lung cancer (NSCLC). We identified burned-out CD8+ TIL subset (Ebo) that specifically accumulated within the tumor microenvironment (TME) but not in adjacent nontumoral tissues. Ebo showed...
The role of tumor-associated macrophages (TAMs) in the cancer immune landscape and their potential as treatment targets or modulators response to are gaining increasing interest. TAMs display high molecular functional complexity. Therefore objective assessment breast biomarkers is critical. aims this study were objectively determine situ expression significance TAM (CD68, CD163, MMP-9) identify subclasses patients who could benefit from TAM-targeting therapies. We measured CD68, MMP-9...
Convalescent plasma (CP), despite limited evidence on its efficacy, is being widely used as a compassionate therapy for hospitalized patients with COVID-19. We aimed to evaluate the efficacy and safety of early CP in COVID-19 progression.The study was an open-label, single-center randomized clinical trial performed academic medical center Santiago, Chile, from May 10, 2020, July 18, final follow-up until August 17, 2020. The included within first 7 days symptom onset, presenting risk factors...
Abstract Purpose: Imaging mass cytometry (IMC) is among the first tools with capacity for multiplex analysis of more than 40 targets, which provides a novel approach to biomarker discovery. Here, we used IMC characterize tumor microenvironment patients metastatic melanoma who received immunotherapy in efforts find indicative factors treatment response. In spite new power IMC, image aspects are still limited by challenges cell segmentation. Experimental Design: rather segment, performed using...
An HIV-1–based pseudotype allows the rapid quantification of neutralizing antibody titers against SARS-CoV-2 spike protein.
Imaging mass cytometry (IMC) uses metal-conjugated antibodies to provide multidimensional, objective measurement of protein targets. We used this high-throughput platform perform an 18-plex assessment HER2 ICD/ECD, cytotoxic T-cell infiltration and other structural signaling proteins in a cohort patients treated with trastuzumab discover associations benefit.An antibody panel for detection 18 targets (pan-cytokeratin, ICD, ECD, CD8, vimentin, cytokeratin 7, β-catenin, HER3, MET, EGFR, ERK...
Porphyromonas gingivalis ( P. ) is a gram-negative oral pathogen associated with chronic periodontitis. Previous studies have linked poor health and periodontitis cancer. Severe cases of periodontal disease can result in advanced periodontitis, leading to tissue degradation, tooth loss, may also correlate higher gastric cancer (GC) risk. In fact, loss an elevated risk However, the clinical evidence for this association remains inconclusive. Periodontitis characterized by inflammation...
In this article, we focus on the fundamental role of vitamin C transporters for normal delivery to germ cells in adluminal compartment seminiferous tubules.We argue that redox status within spermatozoa or semen is partly responsible etiology infertility.In context, antioxidant defence plays a critical male fertility.Vitamin C, micronutrient required wide variety metabolic functions, has long been associated with reproduction.Two systems transport have described mammals.Facilitative hexose...
ABSTRACT The development and survival of male germ cells depend on the antioxidant capacity seminiferous tubule. Glutathione (GSH) plays an important role in defenses spermatogenic epithelium. Autophagy can act as a pro‐survival response during oxidative stress or nutrient deficiency. In this work, we evaluated whether autophagy is involved spermatogonia‐type cell severe GSH We showed that disruption metabolism with l ‐buthionine‐(S,R)‐sulfoximine (BSO) decreased reduced (GSH), oxidized...
Helicobacter pylori (H. pylori) is responsible for causing chronic gastritis, which can cause peptic ulcer and premalignant lesions such as atrophic intestinal metaplasia, dysplasia, with the risk of developing gastric cancer. Recent data describe that H. colonizes mucosa more than 50% world’s population; however, this bacterium has been described infecting human population since its prehistory. This review focuses on populations subpopulations pylori, differentiated by polymorphisms present...
Glycogen is the main source of glucose for many biological events. However, this molecule may have other functions, including those that deleterious effects on cells. The rate-limiting enzyme in glycogen synthesis synthase (GS). It encoded by two genes, GYS1, expressed muscle (muscle synthase, MGS) and tissues, GYS2, primarily liver (liver LGS). Expression GS its activity been widely studied tissues. To date, it not clear which isoform responsible role testis. Using RT-PCR, Western blot...
Dopamine is a catecholamine with multiple physiological functions, playing key role in nervous system; however its participation reproductive processes and sperm physiology controversial. High dopamine concentrations have been reported different portions of the feminine masculine tract, although fulfilled by this as yet unknown. We previously shown that type 2 receptor functional boar sperm, suggesting acts modulator viability, capacitation motility. In present study, using immunodetection...
Abstract Background Convalescent plasma (CP), despite limited evidence on its efficacy, is being widely used as a compassionate therapy for hospitalized patients with COVID-19. We aimed to evaluate the efficacy and safety of early CP in COVID-19 progression. Methods Open-label, single-center, randomized clinical trial performed an academic center Santiago, Chile from May 10, 2020, July 18, final follow-up August 17, 2020. The included within first 7 days symptoms onset, presenting risk...
The histological changes in the gastric epithelium are crucial progression from premalignant to neoplastic lesions. TLR4, TLR5 and TLR9 have been localized studied separately using conventional techniques without a focus on protein or cell interactions within microenvironment. Therefore, we developed multiplex immunohistochemistry/immunofluorescence (mIHC/IF) technology for simultaneous detection of single tissue section human biopsies 10 paired cases collected two independent visits, its...
The main objective of this study was to estimate the performance, under local epidemiological conditions, two in-house ELISA assays for combined detection anti-SARS-CoV-2 IgA, IgM, and IgG immunoglobulins. A total 94 serum samples were used assessment, where 44 corresponded sera collected before pandemic (free SARS-CoV-2 antibodies), 50 from confirmed COVID-19 patients admitted public hospital in city Valdivia, southern Chile. Nucleocapsid (Np) receptor-binding domain (RBD) proteins...
The expression of SYK in cancer cells has been associated with both tumor promoting and suppressive effects. Despite being proposed as anticancer therapeutic target, the possible role modulating local adaptive antitumor immune responses remains uncertain. Using detailed analysis primary human tumors vitro models, we reveal immunomodulatory effect protein solid cancer. We spatially mapped kinase cells, stromal tumor-infiltrating leukocytes (TILs) 808 non-small cell lung carcinomas (NSCLCs)...