A5101751667- RNA modifications and cancer
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Pediatric health and respiratory diseases
- Antibiotic Resistance in Bacteria
- Salmonella and Campylobacter epidemiology
- Bacterial biofilms and quorum sensing
- Immune Response and Inflammation
- Escherichia coli research studies
- Immunotherapy and Immune Responses
- Vibrio bacteria research studies
- Immune Cell Function and Interaction
- HIV Research and Treatment
- RNA regulation and disease
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- Cancer Research and Treatments
- SARS-CoV-2 and COVID-19 Research
- Tryptophan and brain disorders
- Inflammation biomarkers and pathways
- S100 Proteins and Annexins
- HVDC Systems and Fault Protection
- Respiratory viral infections research
- Porphyrin Metabolism and Disorders
- Bacteriophages and microbial interactions
Columbia University
2017-2025
Ludwig-Maximilians-Universität München
2024-2025
University of Chile
2016-2022
Columbia University Irving Medical Center
2018
Pontificia Universidad Católica de Chile
2010-2012
Millennium Institute on Immunology and Immunotherapy
2010-2012
Neutrophils are critical in the host defense against Staphylococcus aureus, a major human pathogen. However, even setting of robust neutrophil response, S. aureus can evade immune clearance. Here, we demonstrate that impairs function by triggering production anti-inflammatory metabolite itaconate. The enzyme synthesizes itaconate, Irg1, is selectively expressed neutrophils during pneumonia. Itaconate inhibits glycolysis and oxidative burst, which survival bacterial killing. In murine...
An HIV-1–based pseudotype allows the rapid quantification of neutralizing antibody titers against SARS-CoV-2 spike protein.
During retroviral replication, the full-length RNA serves both as mRNA and genomic RNA. However, mechanisms by which HIV-1 Gag protein selects two molecules that will be packaged into nascent virions remain poorly understood. Here, we demonstrate deposition of N6-methyladenosine (m6A) regulates packaging. While m6A METTL3/METTL14 onto was associated with increased synthesis reduced packaging, FTO-mediated demethylation promoted incorporation viral particles. Interestingly, associates...
Modifications of RNA, known as the epitranscriptome, affect gene expression, translation, and splicing in eukaryotes, with implications for developmental processes, cancer, viral infections. In prokaryotes, regulation at level epitranscriptome is still poorly understood. Here, we used nanopore direct RNA sequencing Escherichia coli to study modifications their changes under heat stress. With a single reaction, detected most modification types ribosomal (rRNA), transfer (tRNA), messenger...
The social amoeba Dictyostelium discoideum has proven to be a useful model for studying relevant aspects of the host-pathogen interaction. In this work, D. was used as study ability S. Typhimurium survive in amoebae and evaluate contribution selected genes process. To do this, we performed infection assays using axenic cultures co-cultured with wild-type and/or defined mutant strains. Our results confirmed that is able intracellularly discoideum. contrast, mutants ΔaroA ΔwaaL are defective...
IFN-λ and IL-22, cytokines that share the coreceptor IL-10RB, are both induced over course of Klebsiella pneumoniae ST258 (KP35) pneumonia. IL-22 is known to protect mucosal barriers, whereas effects on mucosa not established. We postulated plays a role in regulating airway epithelial barrier facilitate cellular trafficking site infection. In response IFN-λ, transmigration neutrophils across polarized monolayer cells was increased, consistent with diminished integrity. KP35 infection...
Salmonella enterica serovar Typhimurium can enter non-phagocytic cells, such as intestinal epithelial by virtue of a Type Three Secretion System (TTSS) encoded in the Pathogenicity Island 1 (SPI-1), which translocates bacterial effector molecules into host cell. also be taken up dendritic cells (DCs). Although role SPI-1 cell invasion is well established, its contribution to phagocytic has not been evaluated. Here, we have tested invasive capacity S. strain lacking key component TTSS-1...
Inorganic polyphosphate (polyP) deficiency in enteric bacterial pathogens reduces their ability to invade and establish systemic infections different hosts. For instance, inactivation of the kinase gene (ppk) encoding enzyme responsible for polyP biosynthesis invasiveness intracellular survival Salmonella enterica serovar Typhimurium (S. Typhimurium) epithelial cells macrophages vitro. In addition, virulence vivo a S. Δppk mutant is significantly reduced murine infection model. spite these...
causes chronic lung infection in cystic fibrosis (CF), resulting structural damage and progressive pulmonary decline.
Summary Dendritic cells (DCs) are phagocytic professional antigen‐presenting that can prime naive T and initiate anti‐bacterial immunity. However, several pathogenic bacteria have developed virulence mechanisms to impair DC function. For instance, Salmonella enterica serovar Typhimurium prevent DCs from activating antigen‐specific cells. In addition, it has been described the Pathogenicity Island 1 (SPI‐1), which promotes phagocytosis of in non‐phagocytic cells, suppress this process a...
Translation initiation of the human immunodeficiency virus type-1 (HIV-1) full-length RNA has been shown to occur through cap-dependent and IRES-driven mechanisms. Previous studies suggested that nuclear cap-binding complex (CBC) rather than eIF4E drives translation we have recently reported CBC subunit CBP80 supports function viral protein Rev during export this transcript. Ribosome recruitment CBC-dependent cellular mRNAs relies on activity CBP80/20 factor (CTIF), which bridges 40S...
ABSTRACT Modifications of RNA, known as the epitranscriptome, affect gene expression, translation, and splicing in eukaryotes, with implications for developmental processes, cancer, viral infections. In prokaryotes, regulation at level epitranscriptome is still poorly understood. Here, we used Nanopore direct RNA sequencing Escherichia coli to study modifications their changes under heat stress. With a single reaction, detected most modification types rRNA, tRNA, mRNA. was complemented by...
COVID-19 is a pandemic caused by SARS-CoV-2. In Chile, half million people have been infected and more than 16,000 died from COVID-19. As part of the clinical trial NCT04384588, we quantified IgG against S1-RBD SARS-CoV-2 (anti-RBD) in recovered Santiago evaluated their suitability as convalescent plasma donors. ELISA luminescent pseudotype were used for neutralizing antibody quantification. 72.9% population (468 639) showed seroconversion (5-55 μg/mL anti-RBD IgG) suitable candidates...
N6-methyladenosine (m6A) is the most abundant internal modification described in eukaryotic mRNA and several viral RNA including human respiratory syncytial virus (HRSV). Here, we evaluated impact of m6A writers, erasers readers on HRSV genomic accumulation inclusion bodies assembly during replication. We observed that METTL3/METTL14 writer complex plays a negative role protein synthesis titers, while FTO ALKBH5 had opposite effect. also YTHDF1-3 bind to inducing decrease its intracellular...
Abstract N 6 -methyladenosine (m A) is the most abundant internal modification of mRNA in eukaryotes that plays, among other mechanisms, an essential role virus replication. However, understanding m A RNA prokaryotes, especially relation to phage replication, limited. To address this knowledge gap, we investigated effects on replication two model organisms: Vibrio campbellii BAA-1116 (previously V. harveyi BB120) and Escherichia coli MG1655. An A-RNA depleted mutant (Δ rlmF Δ rlmJ ) did not...
-Methyladenosine (m
ABSTRACT During retroviral replication, the full-length RNA serves both as mRNA and genomic (gRNA). While simple retrovirus MLV segregates its into two functional populations, HIV-1 was proposed to exist a single population used indistinctly for protein synthesis or packaging. However, mechanisms by which Gag selects molecules that will be packaged nascent virions remain poorly understood. Here, we demonstrate packaging is regulated through an epitranscriptomic switch requiring demethylation...