A5007971369- Angiogenesis and VEGF in Cancer
- Cancer Research and Treatments
- Reproductive System and Pregnancy
- Ovarian cancer diagnosis and treatment
- Cell Adhesion Molecules Research
- MicroRNA in disease regulation
- Viral-associated cancers and disorders
- Acute Ischemic Stroke Management
- Anatomy and Medical Technology
- Epigenetics and DNA Methylation
- Cancer Genomics and Diagnostics
- Immune cells in cancer
- Chromatin Remodeling and Cancer
- Cancer-related molecular mechanisms research
- Electrospun Nanofibers in Biomedical Applications
- Peripheral Artery Disease Management
- Cerebrovascular and genetic disorders
- Mechanisms of cancer metastasis
- RNA modifications and cancer
- Immunotherapy and Immune Responses
- Nanoplatforms for cancer theranostics
- Tissue Engineering and Regenerative Medicine
University of Pennsylvania
2024-2025
Cancer Research Center
2025
Pontificia Universidad Católica de Chile
2017-2024
The term vasculogenic mimicry (VM) refers to the capacity of certain cancer cells form fluid-conducting structures within a tumor in an endothelial cell (EC)-free manner. Ever since its first report by Maniotis 1999, existence VM has been extremely contentious issue. overwhelming consensus literature suggests that is frequently observed highly aggressive tumors and correlates lower patient survival. While presence vivo animal are claimed upon strong positive staining for glycoproteins...
Long interspersed element 1 (LINE-1) retrotransposons are repetitive sequences that can move within the genome by an autonomous mechanism. To limit their mutagenic potential, benign cells restrict LINE-1 expression through molecular mechanisms such as DNA methylation and histone modification, but these usually impaired in cancer. Clear cell ovarian carcinoma (CCOC) represents 5-10% of cancers is thought to arise from endometriosis. Women with advanced CCOC face poor prognoses, highlighting...
Abstract Vasculogenic mimicry (VM) describes a process by which cancer cells establish an alternative perfusion pathway in endothelial cell-free manner. Despite its strong correlation with reduced patient survival, controversy still surrounds the existence of vitro model VM. Furthermore, many studies that claim to demonstrate VM fail provide solid evidence true hollow channels, raising concerns as whether actual is actually being examined. Herein, we standardized assay recreates formation...
Glioblastoma (GBM) is the most common and aggressive type of brain tumor due to its elevated recurrence following treatments. This mainly mediated by a subpopulation cells with stemness traits termed glioblastoma stem-like (GSCs), which are extremely resistant anti-neoplastic drugs. Thus, an advancement in understanding molecular processes underlying GSC occurrence should contribute significantly towards progress reducing aggressiveness. High levels endothelin-converting enzyme-1 (ECE1), key...
High-Grade Serous Ovarian Cancer (HGSOC) originates from fallopian tube (FT) precursors. However, the molecular changes that occur as precancerous lesions progress to HGSOC are not well understood. To address this, we integrated high-plex imaging and spatial transcriptomics analyze human tissue samples at different stages of development, including p53 signatures, serous tubal intraepithelial carcinomas (STIC), invasive HGSOC. Our findings reveal immune modulating mechanisms within precursor...
Abstract High-Grade Serous Ovarian Cancer (HGSOC) originates from fallopian tube (FT) precursors. However, the molecular changes that occur as precancerous lesions progress to HGSOC are not well understood. To address this, we integrated high-plex imaging and spatial transcriptomics analyze human tissue samples at different stages of development, including p53 signatures, serous tubal intraepithelial carcinomas (STIC), invasive HGSOC. Our findings reveal immune modulating mechanisms within...
Abstract Introduction: Despite the clear genetic evidence linking serous tubal intraepithelial carcinoma (STIC) and high-grade ovarian (HGSOC), specific conditions events that promote progression of STIC lesions into invasive disease remain poorly understood. Method: As a critical initial step, we have assembled cohort incidental p53 signatures, lesions, with concurrent HGSOC. We performed extensive multi-modal analysis using multiplexed tissue imaging spatial transcriptomics identify...
Abstract Introduction: Vasculogenic mimicry (VM) is a clinical phenomenon by which cancer cells can form vessel-like structures in an endothelial-free (CD31-) fashion. VM presence tumors correlates to poor patient prognosis. In our laboratory we have established vitro model where from either cell lines or primary culture lumen-lined and fluid-conducting tubular when grown on laminin-rich Matrigel over four-day period. Given the duration of formation complexity these structures, hypothesize...
Abstract Introduction: A key step in cancer progression is tumor irrigation. The process of angiogenesis can be complemented sub-sets highly aggressive cancers by the vasculogenic mimicry, which formation tubular structures cells. Herein, we sought to establish an vitro assay mimicry and determine percentage ovarian gastrointestinal primary cultures capable undergoing this process. Materials & Methods: Gastric cell lines, AGS Hs746T, lines SKOV-3 HEY were used conjunction with cultured...
Abstract Introduction: The presence of an endothelial cell-free perfusion pathway, known as Vasculogenic Mimicry (VM), strongly correlates with reduced patient survival, however the mechanisms by which a tumor can create self-generated irrigation system remain unknown. Currently there is no mechanism VM formation reported in fluid conducting tubular structures. Herein, using standardized vitro model, we sought to understand pathways required for formation. Methods: We utilized confocal...
Abstract Introduction: Vasculogenic mimicry (VM) describes a process by which cancer cells establish an alternative perfusion pathway in endothelial cell-free manner. Despite the strong correlation with reduced patient survival, mechanisms tumor can create this self-generated irrigation system are still not fully understood. The of VM vitro occur laminin-111-containing Matrigel and requires PI3K pathway. However, membrane protein component signaling pathways involved unknown. Methods: In...
Abstract Introduction: Vasculogenic mimicry (VM) describes a process by which cancer cells establish an alternative perfusion pathway in endothelial cell-free manner. Despite the strong correlation with reduced patient survival, mechanisms tumor can create this self-generated irrigation system are still not fully understood. The of VM vitro only occur Matrigel; however, protein component and signaling pathways involved unknown. Methods: Using established model VM, ovarian breast (HEY...