A5023474509- Ovarian cancer diagnosis and treatment
- Cancer Immunotherapy and Biomarkers
- PARP inhibition in cancer therapy
- COVID-19 Clinical Research Studies
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Cancer-related molecular mechanisms research
- Cancer, Stress, Anesthesia, and Immune Response
- Immune cells in cancer
- Reproductive System and Pregnancy
- Single-cell and spatial transcriptomics
- Viral Infectious Diseases and Gene Expression in Insects
- Ferroptosis and cancer prognosis
- COVID-19 Impact on Reproduction
- CAR-T cell therapy research
- Cytomegalovirus and herpesvirus research
- Integrated Circuits and Semiconductor Failure Analysis
- Maternal and fetal healthcare
- Extracellular vesicles in disease
- Long-Term Effects of COVID-19
- Chromatin Remodeling and Cancer
- RNA modifications and cancer
- Cell death mechanisms and regulation
- RNA Research and Splicing
- Thermal properties of materials
University of Pennsylvania
2019-2025
Cancer Research Center
2019-2025
Penn Center for AIDS Research
2024
Abramson Cancer Center
2024
University of Pennsylvania Health System
2024
Coronavirus disease 2019 (COVID-19) is currently a global pandemic, but human immune responses to the virus remain poorly understood. We used high-dimensional cytometry analyze 125 COVID-19 patients and compare them with recovered healthy individuals. Integrated analysis of ~200 ~50 clinical features revealed activation T cell B subsets in proportion patients. A subgroup had characteristic acute viral infection plasmablast reaching >30% circulating cells. However, another lymphocyte...
Pediatric COVID-19 following SARS-CoV-2 infection is associated with fewer hospitalizations and often milder disease than in adults. A subset of children, however, present Multisystem Inflammatory Syndrome Children (MIS-C) that can lead to vascular complications shock, but rarely death. The immune features MIS-C compared pediatric or adult remain poorly understood. We analyzed peripheral blood responses hospitalized infected patients (pediatric COVID-19) MIS-C. had patterns T cell-biased...
The molecular and functional contributions of intratumoral nerves to disease remain largely unknown. We localized synaptic markers within tumors suggesting that these form connections. Consistent with this, electrophysiological analysis shows malignancies harbor significantly higher electrical activity than benign or normal tissues. also demonstrate pharmacologic silencing tumoral activity. Tumors implanted in transgenic animals lacking nociceptor neurons show reduced These data suggest at...
Abstract Some risk factors for severe coronavirus disease 2019 (COVID-19) have been identified, including age, race, and obesity. However, 20%–50% of cases occur in the absence these factors. Cytomegalovirus (CMV) is a herpesvirus that infects about 50% all individuals worldwide among most significant nongenetic determinants immune system. We hypothesized latent CMV infection might influence severity COVID-19. Our analyses demonstrate seropositivity associated with more than twice...
Single-cell gene expression profiling provides unique opportunities to understand tumor heterogeneity and the microenvironment. Because of cost feasibility, bulk tumors remains primary population-scale analytical strategy. Many algorithms can deconvolve these using single-cell profiles infer their composition. While experimental choices do not change true underlying composition tumor, they affect measurements produced by assay.
The detection of tumor-specific T cells in solid tumors is integral to interrogate endogenous antitumor responses and advance downstream therapeutic applications. Multiple biomarkers are reported identify tumor-infiltrating lymphocytes (TILs), namely CD137, PD-1, CD103, CD39; however, a direct comparison these molecules has yet be performed. We evaluated primary human ovarian tumor samples using single-cell mass cytometry compare their relative phenotypic profiles, examined response...
Long interspersed element 1 (LINE-1) retrotransposons are repetitive sequences that can move within the genome by an autonomous mechanism. To limit their mutagenic potential, benign cells restrict LINE-1 expression through molecular mechanisms such as DNA methylation and histone modification, but these usually impaired in cancer. Clear cell ovarian carcinoma (CCOC) represents 5-10% of cancers is thought to arise from endometriosis. Women with advanced CCOC face poor prognoses, highlighting...
The prognosis of patients diagnosed with advanced ovarian or endometrial cancer remains poor, and effective therapeutic strategies are limited. Müllerian inhibiting substance type 2 receptor (MISIIR) is a transforming growth factor β (TGF-β) family member, overexpressed by most cancers while absent in normal tissues. Restricted tissue expression, coupled an understanding that MISIIR ligation transmits apoptotic signals to cells, makes attractive target for tumor-directed therapeutics....
Theranostics are emerging as a pillar of cancer therapy that enable the use single molecule constructs for diagnostic and therapeutic application. As poly adenosine diphosphate (ADP)-ribose polymerase 1 (PARP-1) is overexpressed in various types, localized to nucleus, PARP-1 can be safely targeted with Auger emitters induce DNA damage tumors. Here, we investigated radioiodinated PARP inhibitor, [125I]KX1, show drug target specific subsequent killing BRCA1 non-BRCA mutant ovarian cells at...
Dense tumor innervation is associated with enhanced cancer progression and poor prognosis. We observed in breast, prostate, pancreatic, lung, liver, ovarian, colon cancers. Defining high-grade serous ovarian carcinoma (HGSOC) was a focus since sensory whereas the normal tissue contains predominantly sympathetic input. The origin, specific nerve type, mechanisms promoting driving nerve-cancer cell communications remain largely unknown. technique of neuro-tracing enhances study by offering...
5510 Background: Currently there are no alternatives to chemotherapy for patients (pts) with platinum-sensitive recurrent high-grade serous ovarian cancer (PSOC). Preclinical data have demonstrated strong synergy between poly (ADP-ribose) polymerase inhibitors (PARPi) and ataxia telangiectasia Rad3-related kinase (ATRi) regardless of homologous recombination deficiency (HRD) status. We present results an investigator-initiated study examining the combination PAPRi ATRi in PSOC. Methods: Pts...
High-Grade Serous Ovarian Cancer (HGSOC) originates from fallopian tube (FT) precursors. However, the molecular changes that occur as precancerous lesions progress to HGSOC are not well understood. To address this, we integrated high-plex imaging and spatial transcriptomics analyze human tissue samples at different stages of development, including p53 signatures, serous tubal intraepithelial carcinomas (STIC), invasive HGSOC. Our findings reveal immune modulating mechanisms within precursor...
Abstract High-Grade Serous Ovarian Cancer (HGSOC) originates from fallopian tube (FT) precursors. However, the molecular changes that occur as precancerous lesions progress to HGSOC are not well understood. To address this, we integrated high-plex imaging and spatial transcriptomics analyze human tissue samples at different stages of development, including p53 signatures, serous tubal intraepithelial carcinomas (STIC), invasive HGSOC. Our findings reveal immune modulating mechanisms within...
Abstract Background Single-cell gene expression profiling provides unique opportunities to understand tumor heterogeneity and the microenvironment. Because of cost feasibility, bulk tumors remains primary population-scale analytical strategy. Many algorithms can deconvolve these using single-cell profiles infer their composition. While experimental choices do not change true underlying composition tumor, they affect measurements produced by assay. Results We generated a dataset high-grade...
ABSTRACT Patients with densely innervated tumors do poorly as compared to those sparsely disease. Why some heavily recruit nerves while others not, remains unknown does the functional contribution of tumor-infiltrating cancer. Moreover, patients receive chemotherapeutic treatment, whether these drugs affect nerve recruitment has not been tested. Using a murine model ovarian cancer, we show that sensory potentiate tumor growth, decrease survival, and contribute treatment resistance....
Abstract The detection of tumor-specific T cells in solid tumors is integral to the interrogation endogenous antitumor responses and advancement downstream therapeutic applications, such as checkpoint immunotherapy adoptive cell transfer. A number biomarkers are reported identify tumor infiltrating lymphocytes (TILs), namely CD137, PD-1, CD103, CD39, however a direct comparison these molecules has yet be performed. Here, we evaluate primary human high-grade serous ovarian samples using...
Background: One of the top five primary causes maternal and newborn death is severe preeclampsia eclampsia.Aim: The aim this study to assess maternity nurses, perception, compliance safety practices among women.Methods: A descriptive cross sectional design was used.This carried out at Menyte El -Nasr Hospital, Delta, Egypt.A purposive non probability sample utilized.The included 107 nurses chosen according inclusion criteria.Three tools were used; structured interview questionnaire, women...
<h3>Background</h3> Clinical data from our group supports that ovarian cancer (OC) patients respond better to immune checkpoint blockade (ICB) when targeting both PD-1 and CTLA4 compared anti-PD-1 alone (33% v. 10%, respectively),<sup>1</sup> yet there is still room improve dual-ICB response. Bevacizumab approved as a front-line therapy for OC targets vascular endothelial growth factor A (VEGF-A). VEGF has been shown have anti-tumor immunomodulatory effects it induces macrophage infiltration...