Sasikanth Manne
A5088868602- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Cancer Immunotherapy and Biomarkers
- CAR-T cell therapy research
- Immune cells in cancer
- Single-cell and spatial transcriptomics
- Complement system in diseases
- Cancer Mechanisms and Therapy
- Cancer Genomics and Diagnostics
- COVID-19 Clinical Research Studies
- IL-33, ST2, and ILC Pathways
- Tuberous Sclerosis Complex Research
- Renal and related cancers
- Asthma and respiratory diseases
- CRISPR and Genetic Engineering
- Viral gastroenteritis research and epidemiology
- Nanoplatforms for cancer theranostics
- Genetic and Kidney Cyst Diseases
- Chemokine receptors and signaling
- Adipokines, Inflammation, and Metabolic Diseases
- Epigenetics and DNA Methylation
- Neuroblastoma Research and Treatments
- Platelet Disorders and Treatments
- Cytomegalovirus and herpesvirus research
Translational Therapeutics (United States)
2019-2025
University of Pennsylvania
2016-2025
Philadelphia University
2023
California University of Pennsylvania
2018-2020
Institute of Immunology
2020
Texas Tech University
2014-2019
Texas Tech University Health Sciences Center
2014-2019
Parker Institute for Cancer Immunotherapy
2019
Iowa State University
2018
Coronavirus disease 2019 (COVID-19) is currently a global pandemic, but human immune responses to the virus remain poorly understood. We used high-dimensional cytometry analyze 125 COVID-19 patients and compare them with recovered healthy individuals. Integrated analysis of ~200 ~50 clinical features revealed activation T cell B subsets in proportion patients. A subgroup had characteristic acute viral infection plasmablast reaching >30% circulating cells. However, another lymphocyte...
Epigenetic profiling suggests that exhausted T cells are a distinct cell linage.
The gut microbiota is a key environmental determinant of mammalian metabolism. Regulation white adipose tissue (WAT) by the process critical to maintaining metabolic fitness, and dysbiosis can contribute development obesity insulin resistance (IR). However, how regulates WAT function remains largely unknown. Here, we show that tryptophan-derived metabolites produced controlled expression miR-181 family in adipocytes mice regulate energy expenditure sensitivity. Moreover, dysregulation...
Abstract The impact of complement on cancer metastasis has not been well studied. In this report, we demonstrate in a preclinical mouse model breast that the anaphylatoxin C5a receptor (C5aR) facilitates by suppressing effector CD8+ and CD4+ T-cell responses lungs. Mechanisms suppression involve recruitment immature myeloid cells to lungs regulation TGFβ IL10 production these cells. favored generation T regulatory (Treg) Th2-oriented rendered dysfunctional. Importantly, pharmacologic...
Current paradigms of CD8 + T cell–mediated protection in HIV infection center almost exclusively on studies peripheral blood, which is thought to provide a window into immune activity at the predominant sites viral replication lymphoid tissues (LTs). Through extensive comparison thoracic duct lymph (TDL), and LTs different species, we show that many LT memory cells bear phenotypic, transcriptional, epigenetic signatures resident (T RMs ). Unlike their circulating counterparts blood or TDL,...
T follicular helper cells (Tfh), a subset of CD4+ cells, provide requisite help to B in the germinal centers (GC) lymphoid tissue. GC Tfh are identified by high expression chemokine receptor CXCR5 and inhibitory molecule PD-1. Although more accessible, blood contains lower frequencies CXCR5+ PD-1+ that have been termed circulating (cTfh). However, it remains unclear whether exit tissues populate this cTfh pool. To examine exiting we assessed phenotype present within major conduit efferent...
Abstract COVID-19 has become a global pandemic. Immune dysregulation been implicated, but immune responses remain poorly understood. We analyzed 71 patients compared to recovered and healthy subjects using high dimensional cytometry. Integrated analysis of ∼200 >30 clinical features revealed activation T cell B subsets, only in some patients. A subgroup had characteristic acute viral infection plasmablast could reach >30% circulating cells. However, another lymphocyte comparable...
The transcription factors T-bet and Eomesodermin (Eomes) regulate CD8 T cell exhaustion through undefined mechanisms. Here, we show that the subcellular localization of Eomes dictate their regulatory activity in exhausted cells (TEXs). TEXs had a higher ratio nuclear Eomes:T-bet than memory (TMEMs) during chronic lymphocytic choriomeningitis virus (LCMV) infection preclinical cancer models human tumors. Biochemically, compete for same DNA sequences, including Pdcd1 T-box. High strongly...