A5014363474- Genomics and Chromatin Dynamics
- Diet and metabolism studies
- Mitochondrial Function and Pathology
- Cancer, Hypoxia, and Metabolism
- Adipose Tissue and Metabolism
- RNA Research and Splicing
- Dermatology and Skin Diseases
- Metabolomics and Mass Spectrometry Studies
- Metabolism, Diabetes, and Cancer
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Immune Response and Inflammation
- T-cell and B-cell Immunology
- Epigenetics and DNA Methylation
- Tryptophan and brain disorders
- Mass Spectrometry Techniques and Applications
- Clostridium difficile and Clostridium perfringens research
- Urticaria and Related Conditions
- Gut microbiota and health
- RNA modifications and cancer
- Psoriasis: Treatment and Pathogenesis
- Adenosine and Purinergic Signaling
- Autoimmune Bullous Skin Diseases
Translational Therapeutics (United States)
2024-2025
University of Pennsylvania
2015-2025
Princeton University
2020-2024
Ludwig Cancer Research
2021-2024
California University of Pennsylvania
2024
Children's Hospital of Philadelphia
2014-2018
University of Chicago
2012-2015
Epigenetic profiling suggests that exhausted T cells are a distinct cell linage.
During mitosis, RNA polymerase II (Pol II) and many transcription factors dissociate from chromatin, ceases globally. Transcription is known to restart in bulk by telophase, but whether de novo at the mitosis-G1 transition any way distinct later interphase remains unknown. We tracked Pol occupancy genome-wide mammalian cells progressing mitosis through late G1. Unexpectedly, during earliest rounds of transition, ∼50% active genes distal enhancers exhibit a spike transcription, exceeding...
Transplantation is the only cure for end-stage organ failure, but without immunosuppression, T cells rapidly reject allografts. While genetic disparities between donor and recipient are major determinants of kinetics transplant rejection, little known about contribution environmental factors. Because colonized organs have worse outcome than sterile organs, we tested influence host microbiota on skin rejection. Compared with untreated conventional mice, pretreatment donors recipients...
Summary / Abstract To maintain lineage-specific functions, cells must acquire and allocate nutrients across diverse cellular processes, even in metabolically-dysregulated environments. The mechanisms allowing CD8+ T to immune function perturbed environments are poorly understood. We find that adapt nutrient stresses over time, reconfiguring gene-regulatory metabolic networks license functional recovery. Under acute stress, reorient translational programming, limiting demand while...
Orbitrap mass spectrometry in full scan mode enables the simultaneous detection of hundreds metabolites and their isotope-labeled forms. Yet, sensitivity remains limiting for many metabolites, including low-concentration species, poor ionizers, low-fractional-abundance forms isotope-tracing studies. Here, we explore selected ion monitoring (SIM) as a means enhancement. The analytes interest are enriched orbitrap analyzer by using quadrupole filter to select particular ions. In tissue...
Abstract Staphylococcus aureus is both a commensal and pathogen, USA300, strain that usually methicillin-resistant but can sometimes be methicillin-susceptible, has been causing skin soft tissue infections (SSTIs) in epidemic proportions among otherwise healthy individuals. Although many people are colonized with S. strains, including some few of these individuals develop SSTIs. This prompts the hypothesis may somewhat reduced innate and/or adaptive immune responses to aureus, either because...
There is considerable interest in investigating the role of microbiota various diseases, including transplant rejection. Germ-free (GF) and gnotobiotic mice are powerful models for this line investigation, but performing surgery within confines a sterile housing isolator exceptionally challenging. Development rigorous protocols to be able remove axenic from their surgical intervention Class II biological safety cabinet (BSC) without compromising sterility would give many investigators access...
Summary The tricarboxylic acid (TCA) cycle oxidizes carbon substrates to dioxide, with the resulting high energy electrons fed into electron transport chain produce ATP by oxidative phosphorylation. Healthy tissues derive most of their from metabolism, and remainder glycolysis. corresponding balance in tumors remains unclear. Tumors upregulate aerobic glycolysis (the Warburg effect), yet they also typically require an intact TCA 1–6 . Recent studies have measured which nutrients contribute...
Orbitrap mass spectrometry in full scan mode enables simultaneous detection of hundreds metabolites and their isotope-labeled forms. Yet sensitivity remains limiting for many metabolites, including low concentration species, poor ionizers, fractional abundance forms isotope tracing studies. Here we explore selected ion monitoring (SIM) as a means enhancement. The analytes interest are enriched the orbitrap analyzer by using quadrupole filter to select particular ions. In tissue extracts, SIM...