A5073654562- Metabolomics and Mass Spectrometry Studies
- Diet and metabolism studies
- Adipose Tissue and Metabolism
- Cancer, Hypoxia, and Metabolism
- Mitochondrial Function and Pathology
- Metabolism, Diabetes, and Cancer
- Mass Spectrometry Techniques and Applications
- Agriculture, Soil, Plant Science
- Mycorrhizal Fungi and Plant Interactions
- Intensive Care Unit Cognitive Disorders
- Biliary and Gastrointestinal Fistulas
- RNA modifications and cancer
- Plant Pathogens and Fungal Diseases
- Clinical Nutrition and Gastroenterology
- Mesenchymal stem cell research
- Esophageal and GI Pathology
- Microbial Metabolic Engineering and Bioproduction
- Food Quality and Safety Studies
- Pediatric Hepatobiliary Diseases and Treatments
- Genomics and Phylogenetic Studies
- Metastasis and carcinoma case studies
- Gastrointestinal disorders and treatments
- Fungal Biology and Applications
- Bone health and treatments
- Genetic Neurodegenerative Diseases
Ludwig Cancer Research
2021-2025
Princeton University
2020-2025
Technion – Israel Institute of Technology
2017-2023
Weatherford College
2023
Presbyterian Medical Center
2021-2022
Seoul National University
2014-2017
Inje University Busan Paik Hospital
2003
The inability to inspect metabolic activities within subcellular compartments has been a major barrier our understanding of eukaryotic cell metabolism. Here, we describe spatial-fluxomics approach for inferring fluxes in mitochondria and cytosol under physiological conditions, combining isotope tracing, rapid fractionation, LC-MS-based metabolomics, computational deconvolution, network modeling. Applied study reductive glutamine metabolism cancer cells, shown mediate fatty acid biosynthesis...
Skeletal fragility is associated with type 2 diabetes mellitus (T2D), but the underlying mechanism not well understood. Here, in a mouse model for youth-onset T2D, we show that both trabecular and cortical bone mass reduced due to diminished osteoblast activity. Stable isotope tracing vivo 13 C-glucose demonstrates glycolysis glucose fueling of TCA cycle are impaired diabetic bones. Similarly, Seahorse assays suppression oxidative phosphorylation by marrow mesenchymal cells as whole, whereas...
Plasma metabolite concentrations and labeling enrichments are common measures of organismal metabolism. In mice, blood is often collected by tail snip sampling. Here, we systematically examined the effect such sampling, relative to gold-standard sampling from an in-dwelling arterial catheter, on plasma metabolomics stable isotope tracing. We find marked differences between circulating metabolome, which arise two major factors: handling stress site, whose effects were deconvoluted taking a...
Reprogramming metabolism is of great therapeutic interest for reducing morbidity and mortality during sepsis-induced critical illness. Disappointing results from randomized controlled trials targeting glutamine antioxidant in patients with sepsis have begged a deeper understanding the tissue-specific metabolic response to sepsis. The current study sought fill this gap. We analyzed skeletal muscle transcriptomics critically ill patients, versus elective surgical controls, which revealed...
Orbitrap mass spectrometry in full scan mode enables the simultaneous detection of hundreds metabolites and their isotope-labeled forms. Yet, sensitivity remains limiting for many metabolites, including low-concentration species, poor ionizers, low-fractional-abundance forms isotope-tracing studies. Here, we explore selected ion monitoring (SIM) as a means enhancement. The analytes interest are enriched orbitrap analyzer by using quadrupole filter to select particular ions. In tissue...
Cancer cells reprogram their metabolism to survive and propagate. Thus, targeting metabolic rewiring in tumors is a promising therapeutic strategy. Genome-wide RNAi CRISPR screens are powerful tools for identifying genes essential cancer cell proliferation survival. Integrating loss-of-function genetic with genomics transcriptomics datasets reveals molecular mechanisms that underlie dependence on specific genes; though explaining line-specific essentiality of was recently shown be especially...
The inability to inspect metabolic activities within distinct subcellular compartments has been a major barrier our understanding of eukaryotic cell metabolism. Previous work addressed this challenge by analyzing metabolism in isolated organelles, which grossly bias activity. Here, we describe method for inferring physiological fluxes and metabolite concentrations mitochondria cytosol based on isotope tracing experiments performed with intact cells. This is made possible computational...
Summary The tricarboxylic acid (TCA) cycle oxidizes carbon substrates to dioxide, with the resulting high energy electrons fed into electron transport chain produce ATP by oxidative phosphorylation. Healthy tissues derive most of their from metabolism, and remainder glycolysis. corresponding balance in tumors remains unclear. Tumors upregulate aerobic glycolysis (the Warburg effect), yet they also typically require an intact TCA 1–6 . Recent studies have measured which nutrients contribute...
Orbitrap mass spectrometry in full scan mode enables simultaneous detection of hundreds metabolites and their isotope-labeled forms. Yet sensitivity remains limiting for many metabolites, including low concentration species, poor ionizers, fractional abundance forms isotope tracing studies. Here we explore selected ion monitoring (SIM) as a means enhancement. The analytes interest are enriched the orbitrap analyzer by using quadrupole filter to select particular ions. In tissue extracts, SIM...
In response to a meal, insulin drives hepatic glycogen synthesis help regulate systemic glucose homeostasis. The mechanistic target of rapamycin complex 1 (mTORC1) is well-established and contributes the postprandial control liver lipid metabolism, autophagy, protein synthesis. However, its role in metabolism less understood. Here, we used metabolomics, isotope tracing, mouse genetics define for mTORC1 signaling glycolytic intermediates deposition. We show that required synthase activity...
Orbitrap mass spectrometry in full scan mode enables simultaneous detection of hundreds metabolites and their isotope-labeled forms. Yet sensitivity remains limiting for many metabolites, including low concentration species, poor ionizers, fractional abundance forms isotope tracing studies. Here we explore selected ion monitoring (SIM) as a means enhancement. The analytes interest are enriched the orbitrap analyzer by using quadrupole filter to select particular ions. In tissue extracts, SIM...
Friedreich's ataxia (FRDA) is a progressive disorder caused by insufficient expression of frataxin, which plays critical role in assembly iron-sulfur centers mitochondria. Individuals are cognitively normal but display loss motor coordination and cardiac abnormalities. Many ultimately develop heart failure. Administration nicotinamide adenine dinucleotide-positive (NAD+) precursors has shown promise human mitochondrial myopathy rodent models failure, including mice lacking frataxin...
Despite decades of study, large parts the mammalian metabolome remain unexplored. Mass spectrometry-based metabolomics routinely detects thousands small molecule-associated peaks within human tissues and biofluids, but typically only a fraction these can be identified, structure elucidation novel metabolites remains low-throughput endeavor. Biochemical language models have transformed interpretation DNA, RNA, protein sequences, not yet had comparable impact on understanding molecule...
Basidiomycetous macrofungi play important roles in maintaining forest ecosystems via carbon cycling and the mobilization of nitrogen phosphorus. To understand impact human activity on macrofungi, an ongoing project at Korea National Arboretum is focused surveying unexploited areas. Mt. Oseo was targeted this survey because number visitors to destination has been steadily increasing, management conservation plans for are urgently required. Through 5 field surveys from April October 2012, 116...
Abstract Skeletal fragility is associated with type 2 diabetes mellitus (T2D), but the underlying mechanism not well understood. Here, in a mouse model for youth-onset T2D, we show that both trabecular and cortical bone mass are reduced due to diminished osteoblast activity. Stable isotope tracing vivo 13 C-glucose demonstrates glycolysis glucose fueling of TCA cycle impaired diabetic bones. Similarly, Seahorse assays suppression oxidative phosphorylation by marrow mesenchymal cells as...
Abstract Pathogenic fungi are a growing health concern worldwide, particularly in large, densely populated cities. The dramatic upsurge of pigeon populations cities has been implicated the increased incidence invasive fungal infections. In this study, we used culture‐independent, high‐throughput sequencing approach to describe diversity clinically relevant ( CRF ) associated with faeces and map relative abundance across Seoul, Korea. addition, tested whether certain geographical,...