A5005552771- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- CRISPR and Genetic Engineering
- RNA Research and Splicing
- Virus-based gene therapy research
- Immune cells in cancer
- CAR-T cell therapy research
- Folate and B Vitamins Research
- Cancer, Hypoxia, and Metabolism
- RNA Interference and Gene Delivery
- Cancer, Stress, Anesthesia, and Immune Response
- Genomics and Chromatin Dynamics
- Hippo pathway signaling and YAP/TAZ
- Gene expression and cancer classification
- Cancer Genomics and Diagnostics
- Wnt/β-catenin signaling in development and cancer
- Genetic Associations and Epidemiology
- Metabolism and Genetic Disorders
- Adipose Tissue and Metabolism
- Cancer-related Molecular Pathways
German Cancer Research Center
2019-2024
Heidelberg University
2019-2024
Weizmann Institute of Science
2023
Technion – Israel Institute of Technology
2020
Medicon Village
2019
Lund University
2017-2019
Science for Life Laboratory
2019
Uppsala University
2019
Accurate classification of breast cancer using gene expression profiles has contributed to a better understanding the biological mechanisms behind disease and paved way for prognostication treatment prediction.We found that miRNA largely recapitulate intrinsic subtypes. In case HER2-enriched tumors small set miRNAs including HER2-encoded mir-4728 identifies group with very high specificity. We also identified differential miR-99a/let-7c/miR-125b cluster as marker separation Luminal A B High...
Abstract Studies of fusion genes have mainly focused on the formation fusions that result in production hybrid proteins or, alternatively, promoter-switching events put a gene under control aberrant signals. However, may also disrupt transcriptional are encoded introns downstream breakpoint. By ignoring structural constraints transcribed fusions, we highlight importance largely unexplored function genes. Here, show, using breast cancer as an example, miRNA host specifically enriched and many...
: Early and aggressive metastasis is a major feature of pancreatic ductal adenocarcinoma. Understanding the processes underlying crucial for making difference to disease outcome. Towards these ends, we looked in comprehensive manner genes that are metastasis-specific.
Binding of transcription factors to mutated DNA sequences is a likely regulator cancer progression. Noncoding regulatory mutations such as those on the core promoter gene encoding human telomerase reverse transcriptase have been shown affect expression in cancer. Using protein microarray 667 factor DNA-binding domains and subsequent functional assays, we looked for that preferentially bind mutant hTERT characterized their downstream effects. One them, friend leukemia integration 1 (FLI1),...
Abstract The combination of lentiviruses with techniques such as CRISPR-Cas9 has resulted in efficient and precise processes for targeted genome modification. An often-limiting aspect, however, is the efficiency cell transduction. Low efficiencies particular types and/or high complexity lentiviral libraries can cause insufficient representation. Here, we present a protocol that yielded substantial increases transduction various lines comparison to several other procedures.
The TP53 gene is mutated in approximately 30% of all breast cancer cases. Adipocytes and preadipocytes, which constitute a substantial fraction the stroma normal mammary tissue tumors, undergo transcriptional, metabolic, phenotypic reprogramming during development play an important role tumor progression. We report here that p53 loss cells facilitates inducing them to acquire unique transcriptional metabolic program combines impaired adipocytic differentiation with augmented cytokine...
MicroRNAs (miRNAs) are ~22-nucleotide-long small non-coding RNAs that regulate the expression of protein-coding genes by base pairing to partially complementary target sites, preferentially located in 3´ untranslated region (UTR) mRNAs. The and function miRNAs have been extensively studied human disease, as well possibility using these molecules biomarkers for prognostication treatment guidance. To identify validate biomarkers, their must be screened large collections patient samples. Here,...
We combined CAGE sequencing in human adipocytes during differentiation with data from genome-wide association studies to identify an enhancer the SNX10 locus on chromosome 7, presumably involved body fat distribution. Using reporter assays and CRISPR-Cas9 gene editing cell lines, we characterized role of adipogenesis. The was active adipogenesis responded strongly insulin isoprenaline. allele associated increased waist-hip ratio genetic higher activity. Mutations resulted less adipocyte...
The core Hippo pathway module consists of a tumour-suppressive kinase cascade that inhibits the transcriptional coactivators Yes-associated protein (YAP) and WW domain-containing transcription regulator 1 (WWTR1; also known as TAZ). When is downregulated, often occurs in breast cancer, YAP/TAZ activity induced. To elaborate roles TAZ triple-negative cancer (TNBC), we depleted Taz murine TNBC 4T1 cells, using either CRISPR/Cas9 or small hairpin RNA (shRNA). TAZ-depleted cells their controls,...