A5089795219- Metabolomics and Mass Spectrometry Studies
- Mass Spectrometry Techniques and Applications
- Bioinformatics and Genomic Networks
- RNA modifications and cancer
- Microbial Metabolic Engineering and Bioproduction
- Molecular Biology Techniques and Applications
- Cancer, Hypoxia, and Metabolism
- Gene expression and cancer classification
- Folate and B Vitamins Research
- Analytical Chemistry and Chromatography
- Advanced Proteomics Techniques and Applications
- Metabolism and Genetic Disorders
Technion – Israel Institute of Technology
2019-2020
Mass spectrometry based metabolomics is a widely used approach in biomedical research. However, current methods coupling mass with chromatography are time-consuming and not suitable for high-throughput analysis of thousands samples. An alternative flow-injection (FI-MS) which samples directly injected to the ionization source. Here, we show that sensitivity Orbitrap FI-MS limited by ion competition effect. We describe an overcoming this effect analyzing distribution m/z values...
Cancer cells reprogram their metabolism to survive and propagate. Thus, targeting metabolic rewiring in tumors is a promising therapeutic strategy. Genome-wide RNAi CRISPR screens are powerful tools for identifying genes essential cancer cell proliferation survival. Integrating loss-of-function genetic with genomics transcriptomics datasets reveals molecular mechanisms that underlie dependence on specific genes; though explaining line-specific essentiality of was recently shown be especially...