A5014255499- Adipose Tissue and Metabolism
- Mitochondrial Function and Pathology
- Genomics and Rare Diseases
- Birth, Development, and Health
- Physiological and biochemical adaptations
- Biochemical and Molecular Research
- Metabolism and Genetic Disorders
- Autophagy in Disease and Therapy
- Genomics and Chromatin Dynamics
- Cancer, Hypoxia, and Metabolism
- Animal Behavior and Reproduction
- Antioxidant Activity and Oxidative Stress
- Effects of Environmental Stressors on Livestock
- Sirtuins and Resveratrol in Medicine
- Adolescent and Pediatric Healthcare
- Genetics, Aging, and Longevity in Model Organisms
- ATP Synthase and ATPases Research
- Viral Infections and Vectors
- Immune Cell Function and Interaction
- Glioma Diagnosis and Treatment
- Endoplasmic Reticulum Stress and Disease
- Epigenetics and DNA Methylation
- Extracellular vesicles in disease
- Diet and metabolism studies
- Vector-borne infectious diseases
Princeton University
2019-2024
Ludwig Cancer Research
2022
Auburn University
2018-2020
Carotenoid coloration is widely recognized as a signal of individual condition in various animals, but despite decades study, the mechanisms that link carotenoid to remain unresolved. Most birds with red feathers convert yellow dietary carotenoids an oxidation process requiring gene encoding putative cytochrome P450 enzyme CYP2J19. Here, we tested hypothesis and feather pigmentation functionally linked mitochondrial performance. Consistent this hypothesis, observed high levels ketolated...
Abstract Many genes that drive normal cellular development also contribute to oncogenesis. Medulloblastoma (MB) tumors likely arise from neuronal progenitors in the cerebellum, and we hypothesized heterogeneity observed MBs with sonic hedgehog (SHH) activation could be due differences developmental pathways. To investigate this question, here perform single-nucleus RNA sequencing on highly differentiated SHH extensively nodular histology malignant cells resembling each stage of canonical...
Detection of small molecule metabolites (SMM), particularly those involved in energy metabolism using MALDI-mass spectrometry imaging (MSI), is challenging due to factors including ion suppression from other analytes present (e.g., proteins and lipids). One potential solution enhance SMM detection remove that cause tissue sections before matrix deposition through solvent washes. Here, we systematically investigated treatment conditions improve signal preserve metabolite localization. Washing...
Diffuse large B-cell lymphomas (DLBCL) are broadly dependent on anaplerotic metabolism regulated by mitochondrial SIRT3. Herein we find that translational upregulation of ATF4 is coupled with in DLBCLs due to nutrient deprivation caused SIRT3 driving rapid flux glutamine into the tricarboxylic acid (TCA) cycle. depletion led downregulation and cell death, which was rescued ectopic expression. Mechanistically, translation inhibited SIRT3-deficient cells increased pools amino acids derived...
Autophagy defects are a risk factor for inflammatory bowel diseases (IBDs) through unknown mechanisms. Whole-body conditional deletion of autophagy-related gene (Atg) Atg7 in adult mice (Atg7Δ/Δ) causes tissue damage and death within 3 mo due to neurodegeneration without substantial effect on intestine. In contrast, we report here that whole-body other essential Atg genes Atg5 or Fip200/Atg17 (Atg5Δ/Δ Fip200Δ/Δ) caused 5 d rapid autophagy inhibition, elimination ileum stem cells, loss...
Understanding of physiological responses organisms is typically based on data collected during an isolated event. Although many fundamental insights have been gained from these studies, evaluating the response to a single event ignores fact that each individual has experienced unique set events throughout its life may altered physiology. The idea prior experiences can influence subsequent performance known as carry-over effect. Carry-over effects explain much variation in found among...
Abstract Carotenoid coloration is widely recognized as a signal of individual condition in various animals, but despite decades study, the mechanisms that link carotenoid to remain unresolved. Most birds with red feathers convert yellow dietary carotenoids an oxidation process requiring gene encoding putative cytochrome P450 enzyme CYP2J19. Here, we tested hypothesis and feather pigmentation functionally linked mitochondrial performance. Consistent this hypothesis, observed high levels...
AbstractAs a major physiological mechanism involved in cellular renewal and repair, immune function is vital to the body's capacity support tissue maintenance organismal survival. Because defenses can be energetically expensive, activities of metabolically active organs, such as liver, are predicted increase during infection by most pathogens. However, some pathogens immunosuppressive, which might reduce metabolic capacities select organs suppress response. Mycoplasma gallisepticum (MG)...
An animal's pace of life is mediated by the physiological demands and stressors it experiences (e.g., reproduction) one likely mechanism that underlies these effects oxidative stress. Reproduction has been shown to increase or reduce stress under different conditions modify mitochondrial performance. We hypothesized changes associated with reproduction can alter how animals respond future stressors. tested this theory comparing organ-specific response in female wild-derived house mice....
Tumor-infiltrating immune cells experience significant metabolic reprogramming in the tumor microenvironment (TME), and they share similar pathways nutrient needs with malignant cells. This positions these cell types direct competition TME. We currently lack a complete understanding of similarities, differences, functional consequences utilized by activated from different lineages versus neoplastic study applies novel situ approach using implantable microdevices to expose 27 controlled...
Abstract Cancer cells need metabolic adaptations to survive in hostile environment such as low nutrient and drug treatment conditions. DLBCLs (Diffuse large B cell lymphoma) originate from a competitive/challenging of germinal center (GC) when undergo positive selections against antigens. However, it is largely unknown whether how flexibility (the ability use different nutrients) plasticity process the same differently) contribute DLBCLs. Our previous study identified that SIRT3, only...
<div>Abstract<p>Diffuse large B-cell lymphomas (DLBCL) are broadly dependent on anaplerotic metabolism regulated by mitochondrial SIRT3. Herein we find that translational upregulation of ATF4 is coupled with in DLBCLs due to nutrient deprivation caused SIRT3 driving rapid flux glutamine into the tricarboxylic acid (TCA) cycle. depletion led downregulation and cell death, which was rescued ectopic expression. Mechanistically, translation inhibited SIRT3-deficient cells increased...
Supplementary Data from Translational Activation of ATF4 through Mitochondrial Anaplerotic Metabolic Pathways Is Required for DLBCL Growth and Survival
Supplementary Data from Translational Activation of ATF4 through Mitochondrial Anaplerotic Metabolic Pathways Is Required for DLBCL Growth and Survival
<div>Abstract<p>Diffuse large B-cell lymphomas (DLBCL) are broadly dependent on anaplerotic metabolism regulated by mitochondrial SIRT3. Herein we find that translational upregulation of ATF4 is coupled with in DLBCLs due to nutrient deprivation caused SIRT3 driving rapid flux glutamine into the tricarboxylic acid (TCA) cycle. depletion led downregulation and cell death, which was rescued ectopic expression. Mechanistically, translation inhibited SIRT3-deficient cells increased...
Supplementary Data from Translational Activation of ATF4 through Mitochondrial Anaplerotic Metabolic Pathways Is Required for DLBCL Growth and Survival
Supplementary Data from Translational Activation of ATF4 through Mitochondrial Anaplerotic Metabolic Pathways Is Required for DLBCL Growth and Survival
Life‐history theory suggests that tradeoffs can occur between self‐maintance and an animal's capacity to allocate resources reproduction. This effect is thought be exacerbated when a female faced with immune challenge during reproductive event. Ultimately, such are curtail lifespan, but the bioenergetic underpinnings of this not well understood. We determined extent any effects in mitochondrial function oxidative damage mice exposed (keyhole limpet haemocyanin) Female ICR were paired male...
Abstract Medulloblastoma (MB) is one of the most common malignant pediatric brain tumors. The sonic hedgehog (SHH) subtype accounts for 30% MB cases and likely arises from mutations in granule cell precursors (GCPs), neuronal progenitors cerebellar cortex that differentiate into neurons. SHH extremely heterogeneous, but it unknown whether this heterogeneity relates to tumors’ developmental origins. To investigate question, we performed single-nucleus RNA-Sequencing on seven highly...