A5073408015- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- SARS-CoV-2 and COVID-19 Research
- Insect Resistance and Genetics
- Immune Cell Function and Interaction
- Viral Infectious Diseases and Gene Expression in Insects
- Glycosylation and Glycoproteins Research
- Long-Term Effects of COVID-19
- Polysaccharides and Plant Cell Walls
- Plant Reproductive Biology
- Psychosomatic Disorders and Their Treatments
- Animal Virus Infections Studies
- COVID-19 Clinical Research Studies
- CAR-T cell therapy research
- Tryptophan and brain disorders
- COVID-19 and Mental Health
- Transgenic Plants and Applications
Children's Hospital of Philadelphia
2024
National Institutes of Health
2021-2023
National Institute of Allergy and Infectious Diseases
2021-2023
North Carolina State University
2018-2020
Background: A substantial proportion of persons who develop COVID-19 report persistent symptoms after acute illness. Various pathophysiologic mechanisms have been implicated in the pathogenesis postacute sequelae SARS-CoV-2 infection (PASC). Objective: To characterize medical and recovery from a cohort disease survivors controls. Design: Cohort study. (ClinicalTrials.gov: NCT04411147) Setting: National Institutes Health Clinical Center, Bethesda, Maryland. Participants: Self-referred adults...
Adaptive immunity requires the expansion of high-affinity lymphocytes from a heterogeneous pool. Whereas current models explain this through signal transduction, we hypothesized that antigen affinity tunes discrete metabolic pathways to license clonal lymphocyte dynamics. Here, identify nicotinamide adenine dinucleotide (NAD) biosynthesis as biochemical hub for T cell receptor affinity-dependent metabolome. Through central anabolic role, found NAD governs quiescence exit checkpoint, thereby...
Messenger RNA (mRNA) vaccines against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are highly effective at inducing protective immunity. However, weak antibody responses seen in some individuals, and cellular correlates of immunity remain poorly defined, especially for B cells. Here we used unbiased approaches to longitudinally dissect primary antibody, plasmablast, memory cell (MBC) the two-dose mRNA-1273 vaccine SARS-CoV-2-naive adults. Coordinated immunoglobulin A (IgA)...
Protective immunity following vaccination is sustained by long-lived antibody-secreting cells and resting memory B (MBCs). Responses to two-dose SARS-CoV-2 mRNA-1273 are evaluated longitudinally multimodal single-cell analysis in three infection-naïve individuals. Integrated surface protein, transcriptomics, cell receptor (BCR) repertoire of sorted plasmablasts spike
Summary / Abstract To maintain lineage-specific functions, cells must acquire and allocate nutrients across diverse cellular processes, even in metabolically-dysregulated environments. The mechanisms allowing CD8+ T to immune function perturbed environments are poorly understood. We find that adapt nutrient stresses over time, reconfiguring gene-regulatory metabolic networks license functional recovery. Under acute stress, reorient translational programming, limiting demand while...
It has been a challenge for scientists to express recombinant secretory eukaryotic proteins structural and biochemical studies. The baculovirus-mediated insect cell expression system is one of the systems used with some post-translational modifications. need be routed through pathways protein glycosylation, disulfide bonds formation, other To improve existing plant proteins, baculovirus vector modified by addition either GP67 or hemolin signal peptide sequence between promoter...
Abstract SARS-CoV-2 mRNA vaccines are highly effective, although weak antibody responses seen in some individuals with correlates of immunity that remain poorly understood. Here we longitudinally dissected antibody, plasmablast, and memory B cell (MBC) to the two-dose Moderna vaccine SARS-CoV-2-uninfected adults. Robust, coordinated IgA IgG were preceded by bursts spike-specific plasmablasts after both doses, but earlier more intensely dose two. Distinct antigen-specific MBC populations also...