A5080424299- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Immunotherapy and Immune Responses
- Advanced biosensing and bioanalysis techniques
- Animal Virus Infections Studies
- HIV Research and Treatment
- SARS-CoV-2 detection and testing
- HIV/AIDS drug development and treatment
- COVID-19 epidemiological studies
- HIV/AIDS Research and Interventions
- Biosensors and Analytical Detection
University of Central Florida
2023-2024
National Institute of Allergy and Infectious Diseases
2022-2023
National Institutes of Health
2022-2023
Protective immunity following vaccination is sustained by long-lived antibody-secreting cells and resting memory B (MBCs). Responses to two-dose SARS-CoV-2 mRNA-1273 are evaluated longitudinally multimodal single-cell analysis in three infection-naïve individuals. Integrated surface protein, transcriptomics, cell receptor (BCR) repertoire of sorted plasmablasts spike
A modular, multi-purpose, and cost-effective electrochemical biosensor based on a five-stranded four-way junction (5S-4WJ) system was developed for SARS-CoV-2 (genes S N) Influenza virus (gene M) detection. The 5S-4WJ structure consists of an electrode-immobilized universal stem-loop (USL) strand, two auxiliary DNA strands, methylene blue redox strand (UMeB). This design allows the detection specific nucleic acid sequences using square wave voltammetry (SWV). sequence-specific strands (m f)...
SARS-CoV-2 mRNA booster vaccines provide protection from severe disease, eliciting strong immunity that is further boosted by previous infection. However, it unclear whether these immune responses are affected the interval between infection and vaccination. Over a two-month period, we evaluated antibody B-cell to third dose vaccine in 66 individuals with different histories. Uninfected post-boost but not previously infected mounted robust ancestral variant spike-binding neutralizing...