A5024631191- Advanced Proteomics Techniques and Applications
- Microbial Metabolic Engineering and Bioproduction
- Mitochondrial Function and Pathology
- Plant Reproductive Biology
- Photosynthetic Processes and Mechanisms
- Mass Spectrometry Techniques and Applications
- Enzyme Structure and Function
- Plant Molecular Biology Research
- Cancer, Hypoxia, and Metabolism
- Genomics and Phylogenetic Studies
- RNA Research and Splicing
- Photoreceptor and optogenetics research
- Molecular Biology Techniques and Applications
- CRISPR and Genetic Engineering
- Protist diversity and phylogeny
- Metabolomics and Mass Spectrometry Studies
- Polysaccharides and Plant Cell Walls
- Nuclear Structure and Function
- Genomics and Chromatin Dynamics
Princeton University
2022-2024
University of Nevada, Reno
2018-2020
Abstract Protein turnover is critical for proteostasis, but quantification challenging, and even in well-studied E. coli , proteome-wide measurements remain scarce. Here, we quantify the rates of ~3200 proteins under 13 conditions by combining heavy isotope labeling with complement reporter ion find that cytoplasmic are recycled when nitrogen limited. We use knockout experiments to assign substrates known ATP-dependent proteases. Surprisingly, none these proteases responsible observed...
Post-translational modifications (PTMs) are critical regulators of protein function, and nearly 200 different types PTM have been identified. Advances in high-resolution mass spectrometry led to the identification an unprecedented number sites numerous organisms, potentially facilitating a more complete understanding how PTMs regulate cellular behavior. While databases created house resulting data, most these resources focus on individual PTM, do not consider quantitative analyses or provide...
Abstract The development of a fertilized egg to an embryo requires the proper temporal control gene expression. During cell differentiation, timing is often controlled via cascades transcription factors (TFs). However, in early development, inactive, and many TF levels stay constant, suggesting that alternative mechanisms govern observed rapid ordered onset Here, we find embryonic access maternally deposited nuclear proteins genome temporally importin affinities, thereby expression...
During pollen-pistil interactions in angiosperms, the male gametophyte (pollen) germinates to produce a pollen tube. To fertilize ovules located within female pistil, tube must physically penetrate specialized tissues. Whereas process of penetration through pistil has been anatomically well described, genetic regulation remains poorly understood. In this study, we identify novel Arabidopsis (Arabidopsis thaliana) gene, O-FUCOSYLTRANSFERASE1 (AtOFT1), which plays key role stigma-style...
Summary Protein turnover is critical for proteostasis, but quantification challenging, and even in well-studied E. coli , proteome-wide measurements remain scarce. Here, we quantify the degradation rates of ∼3.2k proteins under 12 conditions by combining heavy isotope labeling with complement reporter ion find that cytoplasmic are recycled when nitrogen limited. We use knockout experiments to assign substrates known ATP-dependent proteases. Surprisingly, none these proteases responsible...
Abstract Cells face competing metabolic demands. These include efficient use of both limited substrates and proteome capacity, as well flexibility to deal with different environments. Flexibility requires spare enzyme which is inefficient. ATP generation can occur via fermentation or respiration. Fermentation much less substrate-efficient, but often assumed be more 1–3 , thereby favoring fast-growing cells engaging in aerobic glycolysis 4–8 . Here, however, we show that mitochondrial...
Pyrenoids are algal CO
Multiplexed proteomics has become a powerful tool for investigating biological systems. Using balancer-peptide conjugates (e.g., TMTproC complementary ions) in the MS2 spectra quantification circumvents ratio distortion problem inherent multiplexed proteomics. However, scans require long Orbitrap transients and extended ion injection times to achieve sufficient statistics spectral resolution. Real-Time Search (RTS) algorithms have demonstrated increased speed sensitivity by selectively...