A5072009232- Plant nutrient uptake and metabolism
- Polysaccharides and Plant Cell Walls
- Plant Molecular Biology Research
- Plant Reproductive Biology
- Advanced Cellulose Research Studies
- Legume Nitrogen Fixing Symbiosis
- Plant Stress Responses and Tolerance
- Ion Transport and Channel Regulation
- Photosynthetic Processes and Mechanisms
- Aluminum toxicity and tolerance in plants and animals
- Piperaceae Chemical and Biological Studies
- Plant and animal studies
- Insect symbiosis and bacterial influences
- Plant Micronutrient Interactions and Effects
- Nematode management and characterization studies
- Coastal wetland ecosystem dynamics
- Traditional and Medicinal Uses of Annonaceae
- Advanced Proteomics Techniques and Applications
- Ubiquitin and proteasome pathways
- Protein Kinase Regulation and GTPase Signaling
- Healthcare Systems and Challenges
- Membrane-based Ion Separation Techniques
- Species Distribution and Climate Change
- Bioinformatics and Genomic Networks
- 14-3-3 protein interactions
University of Georgia
2024
University of Nevada, Reno
2015-2024
University of California, Berkeley
2012-2014
Energy Biosciences Institute
2012-2014
Queen's University Belfast
2014
Berkeley College
2012
University of Tennessee at Knoxville
2002-2011
Abstract Boron (B) in soil is taken up by roots through NIP5;1, a boric acid channel, and loaded into the xylem BOR1, borate exporter. Here, function of Arabidopsis thaliana NIP6;1, most similar gene to was studied. NIP6;1 facilitates rapid permeation across membrane but completely impermeable water. transcript accumulation elevated response B deprivation shoots not roots. promoter–β-glucuronidase predominantly expressed nodal regions shoots, especially phloem region vascular tissues. Three...
Abstract Major intrinsic proteins (MIPs) are a family of membrane channels that facilitate the bidirectional transport water and small uncharged solutes such as glycerol. The 35 full-length members MIP in Arabidopsis segregated into four structurally homologous subfamilies: plasma (PIPs), tonoplast (TIPs), nodulin 26-like (NIPs), basic (SIPs). Computational methods were used to construct structural models putative pore regions various plant MIPs based on homology modeling with atomic...
Polysaccharide-rich cell walls are a defining feature of plants that influence division and growth, but many details cell-wall organization dynamics unknown because lack suitable chemical probes. Metabolic labeling using sugar analogs compatible with click chemistry has the potential to provide new insights into structure dynamics. Using this approach, we found an alkynylated fucose analog (FucAl) is metabolically incorporated Arabidopsis thaliana roots significant fraction FucAl present in...
Significance Cellulose is the most abundant biopolymer on Earth and a critical component for plants to grow develop. synthesized by large cellulose synthase complexes containing multiple A (CESA) subunits; however, how synthesis regulated remains unclear. In this study, we identify BRASSINOSTEROID INSENSITIVE2 (BIN2) as protein kinase that directly phosphorylates Arabidopsis CESA1 further demonstrate phosphorylation event negatively regulates CESA activity, thus biosynthesis, in . Therefore,...
Soybean nodulin 26 is expressed and targeted to the symbiosome membrane of nitrogen-fixing nodules, where it forms an aquaporin channel with a modest water transport rate. In this study, we show that phosphorylation on Ser-262, which catalyzed by membrane–associated calcium-dependent protein kinase, stimulates its intrinsic Furthermore, using phosphospecific antibody, have elucidated developmental appearance regulation in vivo. Although detected first differentiating infected cells (16...
Major intrinsic proteins (MIPs) are a diverse class of integral membrane that facilitate the transport water and some small solutes across cellular membranes. X-ray structures MIPs indicate tetrad residues (the ar/R region) form narrow pore constriction constitutes selectivity filter. In comparison with mammalian microbial species, plants have greater number diversity than 30 genes encoding four phylogenetic subfamilies eight different classes sequences. The nodulin 26-like protein (NIP)...
Plant nodulin-26 intrinsic proteins (NIPs) are members of the aquaporin superfamily that serve as multifunctional transporters uncharged metabolites. In Arabidopsis thaliana, a specific NIP pore subclass, known II proteins, is represented by AtNIP5;1 and AtNIP6;1, which encode channel expressed in roots leaf nodes, respectively, participate transport critical cell wall nutrient boric acid. Modeling protein encoded AtNIP7;1 gene shows it third member subclass Arabidopsis. However, unlike...
Nodulin 26 (nod26) is a major intrinsic protein that constitutes the component on symbiosome membrane (SM) of N(2)-fixing soybean nodules. Functionally, nod26 forms low energy transport pathway for water, osmolytes, and NH(3) across SM. Besides their functions, emerging evidence suggests high concentrations proteins membranes provide interaction docking targets various cytosolic proteins. Here it shown C-terminal domain peptide interacts with 40-kDa from nodule extracts, which was identified...
Post-translational modifications (PTMs) are critical regulators of protein function, and nearly 200 different types PTM have been identified. Advances in high-resolution mass spectrometry led to the identification an unprecedented number sites numerous organisms, potentially facilitating a more complete understanding how PTMs regulate cellular behavior. While databases created house resulting data, most these resources focus on individual PTM, do not consider quantitative analyses or provide...
Plant cell walls are composed of interlinked polymer networks consisting cellulose, hemicelluloses, pectins, proteins, and lignin. The ordered deposition these components is a dynamic process that critically affects the development differentiation plant cells. However, our understanding wall synthesis remodeling, as well diverse architectures result from processes, has been limited by lack suitable chemical probes compatible with live-cell imaging. In this review, we summarize currently...
The nodulin-like intrinsic protein (NIP) subfamily of water and solute channels in plants is named for nodulin 26 legume nodules. Two NIPs, soybean Lotus japonicus LIMP2, show a distinct functional profile with low osmotic permeability (P(f)) the ability to flux uncharged polyols such as glycerol. NIPs have conserved signature sequence within 'aromatic/arginine' region that forms selectivity filter major proteins. This hybrid glyceroporin aquaporin residues well exhibiting substitutions...
Cellulose is an essential component of plant cell walls and economically important source food, paper, textiles, biofuel. Despite its economic biological significance, the regulation cellulose biosynthesis poorly understood. Phosphorylation dephosphorylation synthases (CESAs) were shown to impact direction velocity synthase complexes (CSCs). However, protein kinases that phosphorylate CESAs are largely unknown. We conducted research in Arabidopsis thaliana reveal CESAs. In this study, we...
During pollen-pistil interactions in angiosperms, the male gametophyte (pollen) germinates to produce a pollen tube. To fertilize ovules located within female pistil, tube must physically penetrate specialized tissues. Whereas process of penetration through pistil has been anatomically well described, genetic regulation remains poorly understood. In this study, we identify novel Arabidopsis (Arabidopsis thaliana) gene, O-FUCOSYLTRANSFERASE1 (AtOFT1), which plays key role stigma-style...
Abstract Studies of herbivores and secondary consumer communities rarely incorporate a comprehensive characterization primary producer trait variation, thus limiting our understanding how plants mediate community assembly consumers. We took advantage recent technological developments for efficient generation phytochemical, microbial genomic data to characterize individual alfalfa ( Medicago sativa ; Fabaceae) growing in an old‐field, semi‐naturalized state 770 traits (including 753 chemical...
The monosaccharide L-fucose (L-Fuc) is a common component of plant cell wall polysaccharides and other glycans, including the hemicellulose xyloglucan, pectic rhamnogalacturonan-I (RG-I) rhamnogalacturonan-II (RG-II), arabinogalactan proteins, N-linked glycans. Mutations compromising biosynthesis many are lethal, as result, small molecule inhibitors polysaccharide have been developed because these molecules can be applied at defined concentrations developmental stages. In this study, we...