A5052676605- Polysaccharides and Plant Cell Walls
- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant nutrient uptake and metabolism
- Biofuel production and bioconversion
- Advanced Cellulose Research Studies
- Microtubule and mitosis dynamics
- Polysaccharides Composition and Applications
- Plant Gene Expression Analysis
- Lignin and Wood Chemistry
- Genetic and Kidney Cyst Diseases
- Glycosylation and Glycoproteins Research
- Plant responses to water stress
- Enzyme-mediated dye degradation
- Plant Stress Responses and Tolerance
- Advanced Fluorescence Microscopy Techniques
- Plant Parasitism and Resistance
- Enzyme Production and Characterization
- Plant tissue culture and regeneration
- Insect-Plant Interactions and Control
- Plant Virus Research Studies
- Insect Pest Control Strategies
- Biochemical and biochemical processes
- Environmental, Ecological, and Cultural Studies
- Tree Root and Stability Studies
Pennsylvania State University
2016-2025
Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale
2024
Energy Biosciences Institute
2009-2012
University of California, Berkeley
2009-2012
Park University
2012
Stanford University
2007-2009
University of North Carolina at Chapel Hill
2003-2004
Coker College
2004
Abertay University
2000
The Ohio State University
1955
Primary cilia are sensory organelles that translate extracellular chemical and mechanical cues into cellular responses. Bone is an exquisitely mechanosensitive organ, its homeostasis depends on the ability of bone cells to sense respond stimuli. One such stimulus dynamic fluid flow, which triggers biochemical transcriptional changes in by unknown mechanism. Here we report possess primary project from cell surface deflect during flow these required for osteogenic resorptive responses flow. We...
Abstract Cellulose forms the major load-bearing network of plant cell wall, which simultaneously protects and directs its growth. Although process cellulose synthesis has been observed, little is known about behavior in wall after synthesis. Using Pontamine Fast Scarlet 4B, a dye that fluoresces preferentially presence excitation emission wavelengths suitable for confocal microscopy, we imaged architecture dynamics walls expanding root cells. We found exists Arabidopsis (Arabidopsis...
Plant cell walls are dynamic structures that synthesized by plants to provide durable coverings for the delicate cells they encase. They made of polysaccharides, proteins, and other biomolecules have evolved withstand large amounts physical force resist external attack herbivores pathogens but can in many cases expand, contract, undergo controlled degradation reconstruction facilitate developmental transitions regulate plant physiology reproduction. Recent advances genetics, microscopy,...
MINI REVIEW article Front. Plant Sci., 27 March 2013Sec. Biotechnology Volume 4 - 2013 | https://doi.org/10.3389/fpls.2013.00067
Pectins are acidic carbohydrates that comprise a significant fraction of the primary walls eudicotyledonous plant cells. They influence wall porosity and extensibility, thus controlling cell organ growth during development. The regulated degradation pectins is required for many separation events in plants, but role pectin expansion poorly defined. Using an activation tag screen designed to isolate genes involved expansion, we identified gene encoding putative polygalacturonase that, when...
Abstract Clathrin-mediated endocytosis (CME) is the best-characterized type of in eukaryotic cells. Plants appear to possess all molecular components necessary carry out CME; however, functional characterization still its infancy. A yeast two-hybrid screen identified μ2 as a putative interaction partner CELLULOSE SYNTHASE6 (CESA6). Arabidopsis (Arabidopsis thaliana) homologous medium subunit 2 mammalian ADAPTOR PROTEIN COMPLEX2 (AP2). In mammals, AP2 complex acts central hub CME by docking...
Xyloglucan constitutes most of the hemicellulose in eudicot primary cell walls and functions wall structure mechanics. Although Arabidopsis (Arabidopsis thaliana) xxt1 xxt2 mutants lacking detectable xyloglucan are viable, they display growth defects that suggestive alterations integrity. To probe mechanisms underlying these defects, we analyzed cellulose arrangement, microtubule patterning dynamics, microtubule- wall-integrity-related gene expression, biosynthesis plants. We found is highly...
Polysaccharide-rich cell walls are a defining feature of plants that influence division and growth, but many details cell-wall organization dynamics unknown because lack suitable chemical probes. Metabolic labeling using sugar analogs compatible with click chemistry has the potential to provide new insights into structure dynamics. Using this approach, we found an alkynylated fucose analog (FucAl) is metabolically incorporated Arabidopsis thaliana roots significant fraction FucAl present in...
Plant cell separation and expansion require pectin degradation by endogenous pectinases such as polygalacturonases, few of which have been functionally characterized. Stomata are a unique system to study both processes because stomatal maturation involves limited between sister guard cells responses reversible elongation contraction. However, the molecular mechanisms for how pores form walls facilitate dynamic remain poorly understood. We characterized POLYGALACTURONASE INVOLVED IN...
Primary cilia, solitary microtubule-based structures that grow from the centriole and extend into extracellular space, have increasingly been implicated as sensors of a variety biochemical biophysical signals. Mutations in primary cilium-related genes linked to number rare developmental disorders well dysregulation cell proliferation. We propose cilia are also important mechanically regulated bone formation adults their malfunction could play role complex multi-factorial diseases, such...
The cell wall consists of cellulose microfibrils embedded within a matrix hemicellulose and pectin. Cellulose are synthesized at the plasma membrane, whereas polysaccharides in Golgi apparatus secreted. trafficking vesicles containing components is thought to depend on actin-myosin. Here, we implicate microtubules this process through studies kinesin-4 family member, Fragile Fiber1 (FRA1). In an fra1-5 knockout mutant, expansion rate inflorescence stem halved compared with wild type along...
Abstract Pectins are abundant in the cell walls of dicotyledonous plants, but how they interact with other wall polymers and influence integrity growth has remained mysterious. Here, we verified that QUASIMODO2 (QUA2) is a pectin methyltransferase determined QUA2 required for normal biosynthesis. To gain further insight into affects assembly maintenance, investigated cellulose biosynthesis, organization, cortical microtubules, signaling two mutant alleles Arabidopsis (Arabidopsis thaliana)...
In Arabidopsis thaliana, lateral roots initiate in a process preceded by periodic gene expression known as the root clock. We identified vesicle-trafficking regulator GNOM and its suppressor, ADENOSINE PHOSPHATE RIBOSYLATION FACTOR GTPase ACTIVATION PROTEIN DOMAIN3, clock regulators. is required for proper distribution of pectin, mediator intercellular adhesion, whereas pectin esterification state essential functional sites primordia emergence, both esterified de-esterified variants are...
Summary Herbivore‐induced plant volatiles (HIPVs) are widely recognized as an ecologically important defensive response of plants against herbivory. Although the induction this ‘cry for help’ has been well documented, only a few studies have investigated inhibition HIPVs by herbivores and little is known about whether evolved mechanisms to inhibit release HIPVs. To examine role herbivore effectors in modulating stomatal dynamics, we conducted series experiments combining pharmacological,...
Abstract Stomatal guard cells are pairs of specialized epidermal that control water and CO2 exchange between the plant environment. To fulfill functions stomatal opening closure driven by changes in turgor pressure, cell walls must be both strong flexible, but how structure dynamics enable function remains poorly understood. address this question, we applied biological genetic analyses to investigate their relationship Arabidopsis (Arabidopsis thaliana). Using live-cell spinning disk...
Lignin is the second most abundant biopolymer on Earth, providing plants with mechanical support in secondary cell walls and defense against abiotic biotic stresses. However, lignin also acts as a barrier to biomass saccharification for biofuel generation (Carroll Somerville, 2009; Zhao Dixon, 2011; Wang et al., 2013 ). For these reasons, studying properties of great interest researchers agriculture bioenergy fields. This protocol describes acetyl bromide method total extraction...