A5019094352- Glutathione Transferases and Polymorphisms
- Genetic and Kidney Cyst Diseases
- Retinal Development and Disorders
- Cellular Mechanics and Interactions
- Growth Hormone and Insulin-like Growth Factors
- Genomics, phytochemicals, and oxidative stress
- Bone Metabolism and Diseases
- Genetic Syndromes and Imprinting
- Photoreceptor and optogenetics research
- Renal and related cancers
- Cell Adhesion Molecules Research
- Cancer Cells and Metastasis
- Cytokine Signaling Pathways and Interactions
- CRISPR and Genetic Engineering
- Tendon Structure and Treatment
- Chemotherapy-induced organ toxicity mitigation
- interferon and immune responses
- IL-33, ST2, and ILC Pathways
- Knee injuries and reconstruction techniques
- Hedgehog Signaling Pathway Studies
- Hippo pathway signaling and YAP/TAZ
- Digestive system and related health
- Alkaline Phosphatase Research Studies
- Photochromic and Fluorescence Chemistry
- Connective tissue disorders research
Australian National University
2011-2024
Australian Cancer Research Foundation
2018-2021
Applied StemCell (United States)
2018
Smith-Kettlewell Eye Research Institute
2010-2011
Oakland University
2010-2011
Rehabilitation Research and Development Service
2007-2010
University of Rochester
2009-2010
VA Palo Alto Health Care System
2004-2009
Veterans Health Administration
2008-2009
Palo Alto Veterans Institute for Research
2007
Primary cilia are sensory organelles that translate extracellular chemical and mechanical cues into cellular responses. Bone is an exquisitely mechanosensitive organ, its homeostasis depends on the ability of bone cells to sense respond stimuli. One such stimulus dynamic fluid flow, which triggers biochemical transcriptional changes in by unknown mechanism. Here we report possess primary project from cell surface deflect during flow these required for osteogenic resorptive responses flow. We...
The success of tissue engineering applications can potentially be dramatically improved with the addition adjuncts that increase proliferation and differentiation progenitor or stem cells. Platelet-rich plasma (PRP) has recently emerged as a potential biologic tool to treat acute chronic tendon disorders. regenerative PRP is based on release growth factors occurs platelet rupture. Its autologous nature gives it significant advantage in applications. To test whether may useful specifically...
Many biochemical factors regulating progenitor cell differentiation have been examined in detail; however, the role of local mechanical environment on stem fate has only recently investigated. In this study, we whether oscillatory fluid flow, an exogenous signal within bone, regulates osteogenic, adipogenic or chondrogenic C3H10T1/2 murine mesenchymal cells by measuring Runx2, PPARgamma and SOX9 gene expression, respectively. Furthermore, hypothesized that small GTPase RhoA isometric tension...
Understanding how the mechanical microenvironment influences cell fate, and more importantly, by what molecular mechanisms, will enhance not only knowledge of mesenchymal stem biology but also field regenerative medicine. Mechanical stimuli, specifically loading induced oscillatory fluid flow, plays a vital role in promoting healthy bone development, homeostasis morphology. Recent studies suggest that such flow has potential to regulate osteogenic differentiation via upregulation multiple...
Primary cilia are chemosensing and mechanosensing organelles that regulate remarkably diverse processes in a variety of cells. We previously showed primary play role mediating bone cells through an unknown mechanism does not involve extracellular Ca(2+)-dependent intracellular Ca(2+) release, which has been implicated all other transduce mechanical signals via the cilium. Here, we identify molecular linking cell mechanotransduction involves adenylyl cyclase 6 (AC6) cAMP. Intracellular cAMP...
The NLRP3 inflammasome is a cytosolic complex sensing phagocytosed material and various damage-associated molecular patterns, triggering production of the pro-inflammatory cytokines interleukin-1 beta (IL)-1β IL-18 promoting pyroptosis. Here, we characterize glutathione transferase omega 1-1 (GSTO1-1), constitutive deglutathionylating enzyme, as regulator inflammasome. Using small molecule inhibitor GSTO1-1 termed C1-27, endogenous knockdown, GSTO1-1-/- mice, report that involved in...
Abstract Glutathione transferase Omega 1 (GSTO1-1) is an atypical GST reported to play a pro-inflammatory role in response LPS. Here we show that genetic knockout of Gsto1 alters the mice three distinct inflammatory disease models. GSTO1-1 deficiency ameliorates stimulated by LPS and attenuates impact high fat diet on glucose tolerance insulin resistance. In contrast, deficient more severe increased escape bacteria from colon into lymphatic system dextran sodium sulfate mediated model bowel...
Cisplatin is an effective anticancer drug; however, cisplatin use often leads to nephrotoxicity, which limits its clinical effectiveness. In this study, we determined the effect of dichloroacetate, a novel agent, in mouse model cisplatin-induced AKI. Pretreatment with dichloroacetate significantly attenuated increase BUN and serum creatinine levels, renal tubular apoptosis, oxidative stress. Additionally, pretreatment accelerated regeneration after damage. Whole transcriptome sequencing...
Whole-exome sequencing of two unrelated kindreds with systemic autoimmune disease featuring antinuclear antibodies IgG4 elevation uncovered an identical ultrarare heterozygous TNIP1
Malnutrition and muscle wasting are common in chronic renal failure (CRF) adversely affect morbidity mortality. Contributing to the is resistance growth hormone (GH). For testing whether impaired GH signaling a cause of skeletal for elucidating its mechanisms, action were studied GH-deficient rats with surgically induced CRF sham-operated pairfed control rats. treatment increased gastrocnemius IGF-1 mRNA levels significantly but not GH-activated Janus-associated kinase 2 (JAK2)-signal...
Neural retina leucine zipper (NRL) is an essential transcription factor for cell fate specification and functional maintenance of rod photoreceptors in the mammalian retina. In Nrl(-/-) mouse retina, photoreceptor precursors fail to produce rods generate cone that predominantly express S-opsin. Previous global expression analysis using microarrays revealed dramatically reduced myocyte enhancer Mef2c adult We undertook this study examine biological relevance retinal photoreceptors....
ABSTRACT Several prophylactic vaccines targeting herpes simplex virus 2 (HSV-2) have failed in the clinic to demonstrate sustained depression of viral shedding or protection from recurrences. Although these generated high titers neutralizing antibodies (NAbs), their induction robust CD8 T cells has largely been unreported, even though evidence for importance HSV-2 antigen-specific is mounting animal models and translational studies involving subjects with active HSV-2-specific immune...
Infection with Helicobacter pylori is known to decrease the level of glutathione in gastric epithelial cells and increase production reactive oxygen species ( ROS ), which can lead DNA damage development cancer. Cation transport regulator 1 CHAC 1) has γ‐glutamylcyclotransferase activity that degrades glutathione. We found cagA ‐positive H. infection triggered overexpression human AGS ) leading degradation accumulation . Nucleotide alterations TP 53 tumour suppressor gene were induced...
Glutathione transferase omega-1 (GSTO1-1) is an enzyme whose function supports the activation of interleukin (IL)-1β and IL-18 that are implicated in a variety inflammatory disease states for which small-molecule inhibitors sought. The potent reactivity active-site cysteine has resulted reported act by covalent labeling. In this study, structure–activity relationship (SAR) elaboration GSTO1-1 inhibitor C1-27 was undertaken. Compounds were evaluated inhibitory activity toward purified...
ABSTRACT. Potassium deficiency (KD) is associated with severe growth failure, in part caused by hormone (GH) resistance. This study set out to determine whether the resistance could be a defect GH-mediated janus kinase–signal transducers and activators of transcription (STAT) signaling as occurs uremia. To this end, rats were fed K-deficient diet for 8 d pair-fed controls received K-replete diet. Animals from each group GH or vehicle, during period, KD resistant; induced body liver weight...