A5036831306- Algal biology and biofuel production
- Microbial Metabolic Engineering and Bioproduction
- Photosynthetic Processes and Mechanisms
- Metabolomics and Mass Spectrometry Studies
- Mitochondrial Function and Pathology
- Microbial Community Ecology and Physiology
- Horticultural and Viticultural Research
- CRISPR and Genetic Engineering
- Cancer, Hypoxia, and Metabolism
- Advanced Proteomics Techniques and Applications
- Enzyme-mediated dye degradation
- Aquatic Ecosystems and Phytoplankton Dynamics
- Bioinformatics and Genomic Networks
- Genomics and Phylogenetic Studies
- Enzyme Catalysis and Immobilization
- Biochemical and biochemical processes
Pennsylvania State University
2018-2024
Oak Ridge National Laboratory
2022
Indian Institute of Technology Bombay
2016-2018
Significance There has been recent interest in fast-growing microbes to accelerate discoveries medicine, biology, and biotechnology. Additionally, with rapid-growth properties are of significant industrial as a chassis for bioproduction. While such have identified, the determinants their rapid growth remain poorly understood. In this study, we elucidated molecular basis Synechococcus 2973, oxygenic photosynthetic organism, applied these insights significantly enhance rate related model...
The factors controlling lignin composition remain unclear. Catechyl (C)-lignin is a homopolymer of caffeyl alcohol with unique properties as biomaterial and precursor industrial chemicals. synthesized in the seed coat Cleome hassleriana switches from guaiacyl (G)- to C-lignin at around 12 14 days after pollination (DAP), associated rerouting monolignol pathway. Lack synthesis limits formation before DAP, but coniferyl still highly accumulated DAP. We propose model which, during biosynthesis,...
ABSTRACT Cyanobacteria are photosynthetic organisms that have garnered significant recognition as potential hosts for sustainable bioproduction. However, their complex regulatory networks pose challenges to major metabolic engineering efforts, thereby limiting feasibility production hosts. Genome streamlining has been demonstrated be a successful approach improving productivity and fitness in heterotrophs but is yet explored its full phototrophs. Here, we present the systematic reduction of...
Cyanobacteria, which constitute a quantitatively dominant phylum, have attracted attention in biofuel applications due to favorable physiological characteristics, high photosynthetic efficiency and amenability genetic manipulations. However, quantitative aspects of cyanobacterial metabolism received limited attention. In the present study, we performed isotopically non-stationary 13 C metabolic flux analysis (INST-13 C-MFA) analyze rerouting carbon glycogen synthase deficient mutant strain...
Synechococcus elongatus UTEX 2973 (Synechococcus 2973) has the shortest reported doubling time (2.1 h) among cyanobacteria, making it a promising platform for solar-based production of biochemicals. In this meta-analysis, its intracellular flux distribution was recomputed using genome-scale isotopic nonstationary 13C-metabolic analysis given labeling dynamics 13 metabolites in an earlier study. To achieve this, mapping model, namely imSyu593, constructed imSyn617 model Synechocystis sp. PCC...
Accurate quantification of mass isotopologue distribution (MID) metabolites is a prerequisite for 13C-metabolic flux analysis. Currently used spectrometric (MS) techniques based on multiple reaction monitoring (MRM) place limitations the number MIDs that can be analyzed in single run. Moreover, deconvolution step results amplification error. Here, we demonstrate SWATH MS/MS, data independent acquisition (DIA) technique allows large precursor and product sequentially fragments all ions...
Abstract Cells face competing metabolic demands. These include efficient use of both limited substrates and proteome capacity, as well flexibility to deal with different environments. Flexibility requires spare enzyme which is inefficient. ATP generation can occur via fermentation or respiration. Fermentation much less substrate-efficient, but often assumed be more 1–3 , thereby favoring fast-growing cells engaging in aerobic glycolysis 4–8 . Here, however, we show that mitochondrial...
Nitrogen fixing-cyanobacteria can significantly improve the economic feasibility of cyanobacterial production processes by eliminating requirement for reduced nitrogen. Anabaena sp. ATCC 33047 is a marine, heterocyst forming, nitrogen fixing cyanobacteria with very short doubling time 3.8 h. We developed comprehensive genome-scale metabolic (GSM) model, iAnC892, this organism using annotations and content obtained from multiple databases. iAnC892 describes both vegetative cell types found in...
Cyanobacteria are photosynthetic organisms that have garnered significant recognition as potential hosts for sustainable bioproduction. However, their complex regulatory networks pose challenges to major metabolic engineering efforts, thereby limiting feasibility production hosts. Genome streamlining has been demonstrated be a successful approach improving productivity and fitness in heterotrophs but is yet explored its full phototrophs. Here we present the systematic reduction of genome...