A5090706571- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Renal Transplantation Outcomes and Treatments
- Organ Transplantation Techniques and Outcomes
- Xenotransplantation and immune response
- Transplantation: Methods and Outcomes
- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- Cytomegalovirus and herpesvirus research
- Pancreatic function and diabetes
- Diabetes and associated disorders
- Reproductive System and Pregnancy
- RNA Interference and Gene Delivery
- Organ and Tissue Transplantation Research
- Pregnancy and Medication Impact
- Biochemical and Molecular Research
- CAR-T cell therapy research
- Antimicrobial Resistance in Staphylococcus
- Animal Genetics and Reproduction
- Diabetes Management and Research
- Dermatology and Skin Diseases
- Antimicrobial Peptides and Activities
- Tissue Engineering and Regenerative Medicine
- Pneumocystis jirovecii pneumonia detection and treatment
- Hematopoietic Stem Cell Transplantation
University of Chicago
2016-2025
GTx (United States)
2024
McGill University Health Centre
2023
Montreal Children's Hospital
2023
Dalhousie University
2023
University of Alberta
2023
University of Nebraska Medical Center
2023
The University of Sydney
2023
NorthShore University HealthSystem
2023
Saint Louis University
2023
Tumor environment can be critical for preventing the immunological destruction of antigenic tumors. We have observed a selective accumulation CD4+CD25+ T cells inside In murine fibrosarcoma Ld-expressing Ag104, these made up majority tumor-infiltrating lymphocytes at late stage tumor progression, and their depletion during effector phase, rather than priming successfully enhanced antitumor immunity. show here that suppressed proliferation interferon-γ production CD8+ in vivo local site....
Glycosylation processes are under high natural selection pressure, presumably because these can modulate resistance to infection. Here, we asked whether inactivation of the UDP-galactose:β-galactoside-α1-3-galactosyltransferase (α1,3GT) gene, which ablated expression Galα1-3Galβ1-4GlcNAc-R (α-gal) glycan and allowed for production anti-α-gal antibodies (Abs) in humans, confers protection against Plasmodium spp. infection, causative agent malaria a major driving force human evolution. We...
Self-assembling peptides and peptide derivatives have received significant interest for several biomedical applications, including tissue engineering, wound healing, cell delivery, drug vaccines. This class of materials has exhibited variability in immunogenicity, with many eliciting no detectable antibody responses but others very strong without any supplemental adjuvants. Presently, strategies either avoiding or specifically inducing them are not well-developed, even though they critical...
Leflunomide, a novel immunosuppressive drug, is able to prevent and reverse allograft xenograft rejection in rodents, dogs, monkeys. It also effective the treatment of several rodent models arthritis autoimmune disease. In vitro studies indicate that leflunomide capable inhibiting anti-CD3- interleukin-2 (IL-2)-stimulated T cell proliferation. However, biochemical mechanism for inhibitory activity has not been elucidated. this study, we characterized effects on Src family (p56lck...
In many experimental models, heart, pancreas and kidney allografts are accepted long-term following costimulation-targeting therapies, whereas skin, lung intestine resist the induction of tolerance under same regimens. We noted that a common feature resistant organs is their constant exposure to commensal microbes hypothesized these microorganisms may stimulate Toll-like receptors (TLRs), promote alloresponses prevent induction. This hypothesis prompts predictions TLR engagement at time...
Ischemic-preconditioning is a process whereby brief ischemic episode confers state of protection against subsequent long-term ischemia-reperfusion injury. Ischemic preconditioning has been studied in heart and liver injury; however, few studies have performed the model preservation-reperfusion injury transplantation. The current study was designed to evaluate ability protect grafts from injury.Male Sprague Dawley rats were used as donors recipients orthotopic done by interruption portal vein...
Epitope content plays a critical role in determining T‐cell and antibody responses to vaccines, biomaterials, protein therapeutics, but its effects are nonlinear difficult isolate. Here, molecular self‐assembly is used build vaccine with precise control over epitope content, order finely tune the magnitude phenotype of T helper responses. Self‐adjuvanting peptide nanofibers formed by co‐assembling high‐affinity universal CD4+ (PADRE) B‐cell from Staphylococcus aureus at specifiable...
ABSTRACT Although many microbial infections elicit an adaptive immune response that can protect against reinfection, it is generally thought Staphylococcus aureus fail to generate protective immunity despite detectable T and B cell responses. No vaccine yet proven prevent S. in humans, efforts develop one have been hampered by a lack of animal models which occurs. Our results describe novel mouse model recurrent infection, skin soft tissue infection (SSTI) strongly protected secondary SSTI...
This work illustrates a strategy for the design of molecularly defined immunotherapies, using blend supramolecular peptide self-assembly and active site-directed protein capture.
Transplantation is the only cure for end-stage organ failure, but without immunosuppression, T cells rapidly reject allografts. While genetic disparities between donor and recipient are major determinants of kinetics transplant rejection, little known about contribution environmental factors. Because colonized organs have worse outcome than sterile organs, we tested influence host microbiota on skin rejection. Compared with untreated conventional mice, pretreatment donors recipients...
Leflunomide is an immunosuppressive drug capable of inhibiting T and B cell responses in vivo. A number studies demonstrate that leflunomide functions both as a pyrimidine synthesis inhibitor tyrosine kinase inhibitor. We previously reported inhibits LPS-stimulated proliferation, cycle progression, IgM secretion. This inhibition can be reversed by the addition exogenous uridine, suggesting cells. report here while uridine restored proliferation secretion to leflunomide-treated cells,...
Abstract The immunosuppressive metabolite of leflunomide, A77 1726, inhibits the enzymatic activity protein tyrosine kinases and dihydro-orotic acid dehydrogenase, an enzyme involved in pyrimidine biosynthesis. Here murine CTLL cell lines were studied to determine which biochemical targets 1726 was responsible for observed inhibition proliferation cytotoxic activity. At low concentrations biosynthesis is target, since correlates with a reduction NTP levels reversed by uridine. higher uridine...
Leflunomide is an immunosuppressive drug capable of inhibiting cellular and humoral mediated responses in vivo. The mechanism responsible for suppression B cell antibody vivo has not been identified. In this study we demonstrate that leflunomide functions to inhibit murine production by directly acting on the cell. Experiments performed showed both T cell-dependent as well cell-independent antigen were suppressed leflunomide. Initial vitro experiments demonstrated inhibited decreasing...