A5003119785- 3D Printing in Biomedical Research
- Immunotherapy and Immune Responses
- Innovative Microfluidic and Catalytic Techniques Innovation
- T-cell and B-cell Immunology
- Microfluidic and Capillary Electrophoresis Applications
- Single-cell and spatial transcriptomics
- Additive Manufacturing and 3D Printing Technologies
- CAR-T cell therapy research
- Immune Response and Inflammation
- Microfluidic and Bio-sensing Technologies
- Monoclonal and Polyclonal Antibodies Research
- Cell Image Analysis Techniques
- Nanofabrication and Lithography Techniques
- Surface Modification and Superhydrophobicity
- Neuroscience and Neural Engineering
- Lymphatic System and Diseases
- RNA Interference and Gene Delivery
- Molecular Junctions and Nanostructures
- Cancer Cells and Metastasis
- Immune Cell Function and Interaction
- Angiogenesis and VEGF in Cancer
- Tissue Engineering and Regenerative Medicine
- vaccines and immunoinformatics approaches
- Electrohydrodynamics and Fluid Dynamics
- Advanced biosensing and bioanalysis techniques
University of Virginia
2016-2025
Carter Center
2018-2025
McCormick (United States)
2016-2024
Virginia College
2021-2024
University of Virginia Cancer Center
2021-2024
Urology of Virginia
2020
Charlottesville Medical Research
2016-2020
University of Chicago
2008-2017
University of Richmond
2005-2007
Columbia University
2004
Epitope content plays a critical role in determining T‐cell and antibody responses to vaccines, biomaterials, protein therapeutics, but its effects are nonlinear difficult isolate. Here, molecular self‐assembly is used build vaccine with precise control over epitope content, order finely tune the magnitude phenotype of T helper responses. Self‐adjuvanting peptide nanofibers formed by co‐assembling high‐affinity universal CD4+ (PADRE) B‐cell from Staphylococcus aureus at specifiable...
The closing of the nuclear fuel cycle is an unsolved problem great importance. Separating radionuclides produced in a reactor useful both for storage waste and recycling fuel. These separations can be performed by designing appropriate chelation chemistries liquid–liquid extraction schemes, such as TALSPEAK process (Trivalent Actinide-Lanthanide Separation Phosphorus reagent Extraction from Aqueous Komplexes). However, there are no approved methods industrial scale reprocessing civilian...
The first microfluidic device for co-culture of two tissue slices under continuous recirculating flow was used to model tumor-induced immunosuppression.
The lymph node is a structurally complex organ of the immune system, whose dynamic cellular arrangements are thought to control much human health. Currently, no methods exist precisely stimulate substructures within or analyze local stimulus-response behaviors, making it difficult rationally design therapies for inflammatory disease. Here we describe novel integration live slices with microfluidic system stimulation. Slices maintained organization while its core experimentally accessible....
The lymph node is a highly organized and dynamic structure that critical for facilitating the intercellular interactions constitute adaptive immunity. Most ex vivo studies of begin by reducing it to cell suspension, thus losing spatial organization, or fixing it, ability make repeated measurements. Live murine tissue slices offer potential retain complexity accessibility, but their viability, level immune activation, retention antigen-specific functions have not been validated. Here we...
ABSTRACT Antibody production is central to protection against new pathogens and cancers, as well certain forms of autoimmunity. Antibodies often originate in the lymph node (LN), specifically at extrafollicular border B cell follicles, where T lymphocytes physically interact drive maturation into antibody-secreting plasmablasts. In vitro models this process are sorely needed predict aspects human immune response. Microphysiological systems (MPSs) offer opportunity approximate lymphoid...
Lymph nodes (LNs) are common sites of metastatic invasion in breast cancer, often preceding spread to distant organs and serving as key indicators clinical disease progression. However, the mechanisms cancer cell into LNs not well understood. Existing vivo models struggle isolate specific impacts tumor-draining lymph node (TDLN) milieu on due coevolving relationship between TDLNs upstream tumor. To address these limitations, we used live ex LN tissue slices with intact chemotactic function...
On-chip 3D culture systems that incorporate immune cells such as lymphocytes and stromal are needed to model organs in engineered organs-on-chip. Photocrosslinking is a useful tool for creating immune-competent hydrogel cultures with spatial cell organization. However, loss of viability motility photocrosslinked gels can limit its utility, especially when working fragile primary cells. We hypothesized optimizing photoexposure-induced ROS production, porosity or combination both factors was...
Enzymatic antibody fragmentation has been well studied for various hosts and isotypes, but patterns also vary unpredictably by clone, optimizing Fab or F(ab′)2 production trial error consumes large quantities of antibodies. Here, we report a systematic strategy functional via pepsin digestion from small IgG. We tested three key parameters that affect fragmentation, pH, enzyme concentration (% w/w), reaction time, found pH had the greatest impact on yield efficiency. then developed approach...
Fluid flow is an integral part of microfluidic and organ-on-chip technology, ideally providing biomimetic fluid, cell, nutrient exchange as well physiological or pathological shear stress. Currently, many the pumps that actively perfuse fluid at rates are incompatible with use inside cell culture incubators, require tubing connections, too large to run devices in a confined space. To address these issues, we developed user-friendly impeller pump uses 3D-printed device recirculate cells...