A5018425784- 3D Printing in Biomedical Research
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Blood groups and transfusion
- Innovative Microfluidic and Catalytic Techniques Innovation
- Erythrocyte Function and Pathophysiology
- Additive Manufacturing and 3D Printing Technologies
- CAR-T cell therapy research
- Diabetes and associated disorders
- Chemokine receptors and signaling
- Spaceflight effects on biology
- Cell Image Analysis Techniques
- Planarian Biology and Electrostimulation
- Cytokine Signaling Pathways and Interactions
- Microfluidic and Bio-sensing Technologies
- RNA regulation and disease
- Single-cell and spatial transcriptomics
- Electrospun Nanofibers in Biomedical Applications
- Atherosclerosis and Cardiovascular Diseases
- Angiogenesis and VEGF in Cancer
- Tissue Engineering and Regenerative Medicine
- Mathematical Biology Tumor Growth
- Cancer Cells and Metastasis
- Blood disorders and treatments
University of Virginia
2016-2025
Massachusetts Institute of Technology
2013
University of Minnesota
2005
ABSTRACT Antibody production is central to protection against new pathogens and cancers, as well certain forms of autoimmunity. Antibodies often originate in the lymph node (LN), specifically at extrafollicular border B cell follicles, where T lymphocytes physically interact drive maturation into antibody-secreting plasmablasts. In vitro models this process are sorely needed predict aspects human immune response. Microphysiological systems (MPSs) offer opportunity approximate lymphoid...
Transfusion-induced anti-red blood cell (RBC) alloantibodies pose a significant risk to patients who require chronic transfusions. Anti-RBC can be remarkably short-lived (i.e. evanescent), leading clinically relevant that are not detected in later pre-transfusion antibody screens. Subsequent transfusion of alloantigen-positive RBCs stimulates rapid memory response may induce delayed hemolytic reaction (DHTR), causing morbidity and occasional mortality chronically transfused patients. It is...
Lymph nodes (LNs) are common sites of metastatic invasion in breast cancer, often preceding spread to distant organs and serving as key indicators clinical disease progression. However, the mechanisms cancer cell into LNs not well understood. Existing vivo models struggle isolate specific impacts tumor-draining lymph node (TDLN) milieu on due coevolving relationship between TDLNs upstream tumor. To address these limitations, we used live ex LN tissue slices with intact chemotactic function...
BACKGROUND: Alloimmunization to transfused red blood cells (RBCs) remains a significant clinical problem. However, the that initiate immune responses RBCs remain incompletely characterized. Recently published work has identified splenic marginal zone B (MZB) as being critically required for production of anti-RBC alloantibodies in response RBCs. In infectious models, MZB cell activation been shown depend on unique population macrophages (MZMs). We hypothesized MZMs would capture stored and...
Prevalent diseases of angiogenesis such as age-related macular degeneration (AMD) exhibit sex-specific prevalence, with female sex being an independent risk factor for the advanced neovascular form AMD. The basis these differences is poorly understood. Here, we quantify impact on suppression aberrant choroidal by human immunoglobulin 1 (hIgG1), which possesses class-wide, antigen-independent anti-angiogenic activity. Males exhibited significantly more robust hIgG1-induced chemotaxis...
On-chip 3D culture systems that incorporate immune cells such as lymphocytes and stromal are needed to model organs in engineered organs-on-chip. Photocrosslinking is a useful tool for creating immune-competent hydrogel cultures with spatial cell organization. However, loss of viability motility photocrosslinked gels can limit its utility, especially when working fragile primary cells. We hypothesized optimizing photoexposure-induced ROS production, porosity or combination both factors was...
IL-2 and IL-15 are common γ-chain family cytokines involved in regulation of T cell differentiation homeostasis. Despite signaling through the same receptors, have non-redundant roles biology, both physiologically at cellular level. The mechanisms by which trigger distinct phenotypes cells remain elusive. To elucidate these mechanisms, we performed a quantitative comparison phosphotyrosine network resulting triggered IL-15. This study revealed that networks activated or highly similar...
We present an easily applicable and inexpensive method for patterning cells on arbitrary surfaces including biological gels with little loss of viability or function. Single-cell suspensions human umbilical vein endothelial NIH 3T3 fibroblasts were sprayed off-the-shelf airbrush through a mask to create 100-µm scale patterns collagen gels. Three-dimensional created by layering gel top the first pattern gel. Coculture rat hepatocytes resulted in localized increased activity cytochrome P-450...
Abstract Anemias of chronic disease and inflammation (ACDI) result from restricted iron delivery to erythroid progenitors. The current studies reveal an organellar response in restriction consisting disassembly the microtubule cytoskeleton associated Golgi disruption. Isocitrate supplementation, known abrogate response, induces reassembly microtubules deprived Ferritin, based on proteomic profiles, regulation by isocitrate, putative interaction with microtubules, is assessed as a candidate...
Lymph nodes (LNs) are common sites of metastatic invasion in breast cancer, often preceding spread to distant organs and serving as key indicators clinical disease progression. However, the mechanisms cancer cell into LNs not well understood. Existing vivo models struggle isolate specific impacts tumor-draining lymph node (TDLN) milieu on due co-evolving relationship between TDLNs upstream tumor. To address these limitations, we used live ex LN tissue slices with intact chemotactic function...
The lymph node (LN) performs essential roles in immunosurveillance throughout the body. Developing vitro models of this key tissue is great importance to enhancing physiological relevance immunoengineering. LN consists stromal populations and immune cells, which are highly organized bathed constant interstitial flow. stroma, notably fibroblastic reticular cells (FRCs) lymphatic endothelial (LECs), play crucial guiding T cell migration known be sensitive fluid During inflammation, flow rates...
Bubbles are a common cause of microfluidic malfunction, as they can perturb the fluid flow within micro-sized features device. Since gas bubbles form easily warm cell culture reagents, degassing is often necessary for biomicrofluidic systems. However, fabrication microscale degasser that be used modularly with pre-existing chips may cumbersome or challenging, especially labs not equipped traditional microfabrication, and current commercial options expensive. Here, we address need an...
Studies of human patients have shown that most anti-RBC alloantibodies are IgG1 or IgG3 subclasses, although it is unclear why transfused RBCs preferentially drive these subclasses over others. Though mouse models allow for the mechanistic exploration class-switching, previous studies RBC alloimmunization in mice focused more on total IgG response than relative distribution, abundance, mechanism subclass generation. Given this major gap, we compared distribution generated to protein alum...
Abstract Micropatterning techniques for 3D cell cultures enable the recreation of tissue-level structures, but their combination with well-defined, microscale fluidic systems perfusion remains challenging. To address this technological gap, we developed a user-friendly in-situ micropatterning protocol that integrates photolithography crosslinkable, cell-laden hydrogels simple microfluidic housing, and tested impact crosslinking chemistry on stability spatial resolution. Working gelatin...
Studies of human patients have shown that most anti-RBC alloantibodies are IgG1 or IgG3 subclasses, though it is unclear why transfused RBCs preferentially drive these subclasses over others. Though mouse models allow for the mechanistic exploration class-switching, previous studies RBC alloimmunization in mice focused more on total IgG response than relative distribution, abundance, mechanism subclass generation. Given this major gap, we compared distribution generated to protein alum...
Abstract IL-2 and IL-15 signal through a heterotrimeric surface receptor complex (IL-2/15R) composed of an α, β, the common gamma (γc) chain. Signaling occurs shared β γc chains, α-chains function as high affinity ligand capture agents. Despite sharing signaling chains pathways, have non-redundant roles in T cell biology. In vitro, although equivalent mitogens, they greatly differ their activities growth factors. cells cultured with are larger size, show increased protein synthesis,...
Abstract Alloimmunization to non-ABO red blood cell (RBC) antigens remains a significant clinical problem in transfusion medicine. Presence of these alloantibodies can lead delayed hemolytic reactions resulting morbidity and occasional mortality upon antigenic re-exposure. For patients that generate multiple alloantibodies, provision compatible antigen-negative RBCs be time resource intensive. In rare cases, it result the inability locate provide life-saving therapy. Despite consequences RBC...