A5003683161- Renal Transplantation Outcomes and Treatments
- T-cell and B-cell Immunology
- Transplantation: Methods and Outcomes
- Immune Cell Function and Interaction
- Organ Transplantation Techniques and Outcomes
- Immunotherapy and Immune Responses
- Cytomegalovirus and herpesvirus research
- Monoclonal and Polyclonal Antibodies Research
- SARS-CoV-2 and COVID-19 Research
- Cancer Genomics and Diagnostics
- Viral Infections and Immunology Research
- Pneumocystis jirovecii pneumonia detection and treatment
- Platelet Disorders and Treatments
- Phagocytosis and Immune Regulation
- Genetic factors in colorectal cancer
- SARS-CoV-2 detection and testing
- Immunodeficiency and Autoimmune Disorders
- Renin-Angiotensin System Studies
- Blood groups and transfusion
- Renal Diseases and Glomerulopathies
- Adenosine and Purinergic Signaling
- Scientific Computing and Data Management
- Organ Donation and Transplantation
- Medical and Biological Sciences
- COVID-19 Clinical Research Studies
Northwestern University
2017-2024
Emory University
2012-2020
RELX Group (United States)
2020
Woodruff Health Sciences Center
2015
Currently, the ability to predict or monitor efficacy of HLA antibody–removal therapies is deficient. We previously reported that titration studies are a consistent and accurate means assessing antibody strength. To test whether can also which patients better candidates for desensitization, we studied 38 from 3 centers (29 receiving plasmapheresis/low-dose intravenous immunoglobulin [IVIg]; 9 high-dose IVIg). For undergoing IVIg, titer reduction correlated with number treatment cycles both...
Molecular mismatch load analysis was recently introduced as a means for performing risk stratification following organ transplantation. However, although good correlation demonstrated between molecular and generation of de novo donor-specific HLA antibody (DSA), quite few exceptions exist, the underlying factors that define immunogenicity remain unclear. Herein, we present new paradigm to interrogate differences mismatches lead DSA those do not (the 2MM1DSA cohort). Specifically, patients...
Abstract Blockade of the CD40/CD154 pathway remains one most effective means promoting graft survival following transplantation. However, effects antagonism on dendritic cell (DC) phenotype and functionality transplantation remain incompletely understood. To dissect CD154/CD40 blockade DC activation in vivo, we generated hematopoietic chimeras mice that expressed a surrogate minor Ag (OVA). Adoptive transfer OVA-specific CD4+ CD8+ T cells led to chimerism rejection, which was inhibited by...
Donor-reactive memory T cells generated via heterologous immunity represent a potent barrier to long-term graft survival following transplantation because of their increased precursor frequency, rapid effector function, altered trafficking patterns, and reduced reliance on costimulation signals for activation. Thus, the identification pathways that control cell secondary recall potential may provide new opportunities therapeutic intervention. Here, we discovered donor-specific...
Latent viral infections are a major concern among immunosuppressed transplant patients. During clinical trials with belatacept, CTLA4-Ig fusion protein, patients showed an increased risk of Epstein-Barr virus-associated posttransplant lymphoproliferative disorder, thought to be due deficient primary CD8(+) T cell response the virus. Using murine model latent infection, we observed that rapamycin treatment alone led significant increase in virus-specific cells, as well functionality these...
Despite ubiquitous exposure to sensitizing events, most Fontan PLE patients have low panel reactive antibodies (PRA). To assess whether they are at risk for donor-specific antibody (DSA) memory response following heart transplantation (HT) when their resolves, DSA profiles, incidence of rejection, and graft outcomes in recipients with without were compared.Patient characteristics, appearance newly detected (nDSA), compared between using Wilcoxon rank-sum Chi-squared tests. burden was...
T cells have the remarkable ability to recognize antigen with great specificity and in turn mount an appropriate robust immune response. Critical this process is initial cell recognition subsequent signal transduction events. This can be modulated at site of TCR interaction peptide:major histocompatibility (pMHC) or peptide MHC molecule. Both events could a range effects on fate. Though responses antigens that bind sub-optimally TCR, known as altered ligands (APL), been studied extensively,...
Signal-regulatory protein α (SIRPα), a polymorphic inhibitory membrane-bound receptor, and its ligand CD47 have recently been implicated in the modulation of innate immune allorecognition murine models. Here, we investigate potential impact SIRPα donor-recipient mismatches on graft outcomes human kidney transplantation. To eliminate specific role HLA-matching alloresponse, genotyped two most common variants cohort 55 HLA-identical, biologically-related, pairs. 69% pairs were identical. No...
Solid organ transplant recipients with immunosuppressant regimens to prevent rejection are less able mount effective immune responses pathogenic infection. Here, we apply a recently reported mass spectrometry-based serological approach known as Ig-MS characterize against infection SARS-CoV-2 in cohorts of and immunocompetent controls, both at single early time point following COVID-19 diagnosis well over the course one-month postdiagnosis. We found that antibody repertoires generated by do...
ABSTRACT Virtual crossmatch (VXM) compares a transplant candidate’s unacceptable antigens to the HLA typing of donor before an organ offer is accepted and, in selected cases, supplant prospective physical crossmatch. However, deceased can be ambiguous, leading uncertainty compatibility prediction. We have developed web application that utilizes ambiguous molecular data assist VXM assessments. The listed computed probabilities all possible two-field alleles and UNOS antigens. VIrtual...