A5013625442- Mesenchymal stem cell research
- Gut microbiota and health
- Immune Cell Function and Interaction
- RNA Interference and Gene Delivery
- Immune cells in cancer
- Immunotherapy and Immune Responses
- Hematopoietic Stem Cell Transplantation
- Neuroinflammation and Neurodegeneration Mechanisms
- Gastrointestinal motility and disorders
- Vagus Nerve Stimulation Research
- Inflammatory Bowel Disease
- Pancreatic function and diabetes
- Tissue Engineering and Regenerative Medicine
- Inflammasome and immune disorders
- IL-33, ST2, and ILC Pathways
- Cancer Cells and Metastasis
- MicroRNA in disease regulation
- Single-cell and spatial transcriptomics
- Alzheimer's disease research and treatments
- Pluripotent Stem Cells Research
- Pediatric health and respiratory diseases
- Infant Health and Development
- Tryptophan and brain disorders
- Bone fractures and treatments
- T-cell and B-cell Immunology
Harvard University
2019-2024
Boston VA Research Institute
2023-2024
Massachusetts General Hospital
2024
Brigham and Women's Hospital
2019-2024
The University of Adelaide
2014-2020
RELX Group (United States)
2018-2020
Royal Adelaide Hospital
2014-2019
University of South Australia
2018
Centre for Cancer Biology
2015
Interferon-γ (IFN-γ)-preactivated mesenchymal stem cells (MSC-γ) are highly immunosuppressive but immunogenic in vivo due to their inherent expression of major histocompatibility (MHC) molecules. Here, we present an improved approach where modified human bone marrow-derived MSC with interleukin-17A (MSC-17) enhance T cell immunosuppression not immunogenicity. MSC-17, unlike MSC-γ, showed no induction or upregulation MHC class I, II, and costimulatory molecule CD40, maintained normal...
Porous silicon nanoparticles (pSiNP), modified to target dendritic cells (DC), provide an alternate strategy for the delivery of immunosuppressive drugs. Here, we aimed develop a DC-targeting pSiNP displaying c-type lectin, cell-specific intercellular adhesion molecule-3-grabbing non-integrin (DC-SIGN), and CD11c monoclonal antibodies. The in vivo tracking these fluorescent was assessed both C57BL/6 mice common marmosets (Callithrix jacchus) by intravenous injection (20 mg/kg). Rapamycin...
ABSTRACT MSC‐like populations derived from induced pluripotent stem cells (iPSC‐MSC) serve as an alternative cell source due to their high proliferative capacity. In this study, we assessed the immunomodulatory potential of iPSC‐MSC generated periodontal ligament (PDL) and gingival (GF) tissue. The lines exhibited a similar level suppression mitogen‐stimulated peripheral blood mononuclear (PBMNC) proliferation compared respective parental fibroblast in vitro. Moreover, demonstrated ability...
Human mesenchymal stem cells pretreatment with IL-17A (MSC-17) potently enhances T cell immunosuppression but not their immunogenicity, in addition to avidly promoting the induction of suppressive regulatory cells. The aim this study was identify potential mechanisms by which human MSC-17 mediate superior immunomodulatory function. Untreated-MSC (UT-MSC), IFN-γ treated MSC (MSC-γ), and were assessed for gene expression profile microarray. Significantly regulated genes identified biological...
Gut inflammation involves contributions from immune and non-immune cells, whose interactions are shaped by the spatial organization of healthy gut its remodeling during inflammation. The crosstalk between fibroblasts cells is an important axis in this process, but our understanding has been challenged incomplete cell-type definition biogeography. To address challenge, we used MERFISH to profile expression 940 genes 1.35 million imaged across onset recovery a mouse colitis model. We...
Compact bones (CB) are major reservoirs of mouse mesenchymal stem cells (mMSC). Here, we established a protocol to isolate MSC from CB and tested their immunosuppressive potential. Collagenase type II digestion BM-flushed C57B/6 mice was performed liberate mMSC precursors bone surfaces establish nondepleted mMSC. were also immunodepleted based on the expression CD45 (leukocytes) TER119 (erythroid cells) eliminate hematopoietic cells. CD45-TER119- subsequently used generate depleted...
SUMMARY Gut-innervating nociceptor sensory neurons respond to noxious/tissue-damaging stimuli by initiating protective responses and releasing mediators that regulate tissue inflammation, gastrointestinal secretion, motility. The role of nociceptors in host defense against enteric pathogens is unclear. Here, we found gut-extrinsic are critical protecting the Salmonella typhimurium (STm) infection. Nociceptors responded STm neuropeptide calcitonin gene-related peptide (CGRP). Targeted...
Background Adult mesenchymal stem cells (MSC) are a promising avenue to generate insulin-secreting beta replace the need for large numbers of human islets required transplantation. In this preliminary study, we aim induce expression endodermal markers FoxA2 and Sox17 in MSC by priming them with demethylation agents as strategy enhance efficiency differentiation into cells. Methods Human bone marrow derived were isolated cultured from 3 healthy donors. either untreated (untreated-MSC) or...
MSC have immunosuppressive properties beneficial for transplantation cellular therapy. We modified human bone marrow-derived with inflammatory cytokines as a strategy to enhance immunosuppression on T cells. were preconditioned IFN-γ, TNF-α, IL-1β, IL-2, IL-12 and IL-17 5d co-cultured cells activated by the mitogen phytohemagglutinin (PHA). Modified displayed greater cell proliferation compared unmodified-MSC (UT:MSC). IL-6 gene expression significantly increased in TNF-α IL-1β-modified...