A5002941103- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Renal Transplantation Outcomes and Treatments
- Renal Diseases and Glomerulopathies
- Neuroscience and Neuropharmacology Research
- Receptor Mechanisms and Signaling
- interferon and immune responses
- Influenza Virus Research Studies
- Ion channel regulation and function
- Cytomegalovirus and herpesvirus research
- Single-cell and spatial transcriptomics
- Cardiac electrophysiology and arrhythmias
- Complement system in diseases
- Adenosine and Purinergic Signaling
- Organ Transplantation Techniques and Outcomes
- Liver Disease Diagnosis and Treatment
- Neuroinflammation and Neurodegeneration Mechanisms
- COVID-19 Clinical Research Studies
- Immune cells in cancer
- Cytokine Signaling Pathways and Interactions
- Liver Diseases and Immunity
- Systemic Lupus Erythematosus Research
- Electrochemical Analysis and Applications
- Monoclonal and Polyclonal Antibodies Research
- Respiratory viral infections research
Icahn School of Medicine at Mount Sinai
2015-2024
Mount Sinai Hospital
2024
Universidad Politécnica de Madrid
2018
Brooklyn Hospital Center
1954
Kidney glomerulosclerosis commonly progresses to end-stage kidney failure, but pathogenic mechanisms are still poorly understood. Here, we show that podocyte expression of decay-accelerating factor (DAF/CD55), a complement C3 convertase regulator, crucially controls disease in murine models adriamycin (ADR)-induced focal and segmental (FSGS) streptozotocin (STZ)-induced diabetic glomerulosclerosis. ADR induces enzymatic cleavage DAF from surfaces, leading activation. deficiency or prevention...
Early interactions of influenza A virus (IAV) with respiratory epithelium might determine the outcome infection. The study global cellular innate immune responses often masks multiple aspects mechanisms by which populations cells work as organized and heterogeneous systems to defeat infection, how counteracts these systems. In this study, we experimentally dissected dynamics IAV human epithelial cell interaction during early infection at single-cell level. We found that number viruses...
Signals produced by early responding cells to viral infection calibrate the immune response of cell population.
Although spontaneous kidney transplant acceptance/tolerance occurs in mice and occasionally humans, mechanisms remain unclear. Herein we test the hypothesis that EPO, a hormone predominantly produced by adult kidney, has immunomodulating properties are required for graft acceptance. In vitro , manner dependent on EPO receptor CD131 antigen-presenting cells, induced secretion of active TGF β which turn converted naïve CD4 + T cells into functional Foxp3 regulatory (Treg). murine models,...
Kidney transplant recipients (KTRs) show poorer response to SARS-CoV-2 mRNA vaccination, yet patterns and mechanistic drivers following third doses are ill-defined. We administered monovalent vaccines n = 81 KTRs with negative or low-titer anti-receptor binding domain (RBD) antibody (n 39 anti-RBD
Membrane trafficking processes regulate G protein-coupled receptor (GPCR) activity. Although class A GPCRs are capable of activating proteins in a monomeric form, they can also potentially assemble into functional GPCR heteromers. Here, we showed that the serotonin 5-HT2A receptors (5-HT2ARs) affected localization and C metabotropic glutamate 2 (mGluR2) through mechanism required their assembly as heteromers mammalian cells. In absence agonists, 5-HT2AR was primarily localized within...
B cell depleting therapies permit immunosuppressive drug withdrawal and maintain remission in patients with frequently relapsing nephrotic syndrome (FRNS) or steroid–dependent (SDNS), but lack of biomarkers for treatment failure. Post-depletion immune reconstitution may identify patients, previous characterizations suffered from methodological limitations flow cytometry. Time-of-flight mass cytometry (CyTOF) is a comprehensive analytic modality that simultaneously quantifies over 40 cellular...
Immune cell metabolism plays a pivotal role in shaping and modulating immune responses. The metabolic state of cells influences their development, activation, differentiation, overall function, impacting both innate adaptive immunity. While glycolysis is crucial for activation effector function CD8 T cells, regulatory mainly use oxidative phosphorylation fatty acid oxidation, highlighting how different programs shape cells. Modification may provide new therapeutic approaches to prevent...
A large number of ion channels maintain their activity through direct interactions with phosphatidylinositol bisphosphate (PIP2). For such channels, hydrolysis PIP2 causes current inhibition. It has become controversial whether the inhibitory effects on channel represent or downstream PKC action. We studied Phospholipase C (PLC)-dependent inhibition G protein-activated inwardly rectifying K+ (Kir3) channels. By monitoring simultaneously and hydrolysis, we determined that both depletion...
Costimulatory blockade-induced murine cardiac allograft survival requires intragraft accumulation of CD11b+Ly6CloLy6G− regulatory myeloid cells (Mregs) that expand T (Tregs) and suppress effector (Teffs). We previously showed C5a receptor (C5aR1) signaling on activates Teffs inhibits Tregs, but whether and/or how C5aR1 affects Mregs required for transplant is unknown. Although BALB/c hearts survived >60 days in anti-CD154 (MR1)-treated or cytotoxic T-lymphocyte associated protein 4...
CD4+ follicular helper T (Tfh) cells are specialized providers of cell help to B and can function as pathogenic mediators murine antibody-dependent chronic graft-versus-host disease (GvHD). Using a parent→F1 model lupus-like GvHD, in which Tfh germinal center (GC) differentiation occurs over 14 days, we demonstrate that absence cell–expressed C5a receptor 1 (C5ar1) or pharmacological C5aR1 blockade abrogated generation/expansion cells, GC autoantibodies. In cell–dependent GvHD manifested by...
Abstract Mammalian red blood cells (RBCs), which primarily contain hemoglobin, exemplify an elaborate maturation process, with the terminal steps of RBC generation involving extensive cellular remodeling. This encompasses alterations content through distinct stages erythroblast that result in expulsion nucleus (enucleation) followed by loss mitochondria and all other organelles a transition to anaerobic glycolysis. Whether there is any link between erythroid removal function organelle,...
ABSTRACT Influenza viruses continue to present global threats human health. Antigenic drift and shift, genetic reassortment, cross-species transmission generate new strains with differences in epidemiology clinical severity. We compared the temporal transcriptional responses of dendritic cells (DC) infection two pandemic (A/Brevig Mission/1/1918, A/California/4/2009) seasonal (A/New Caledonia/20/1999, A/Texas/36/1991) H1N1 influenza viruses. Strain-specific response included stronger...
Abstract C5aR2 (C5L2/gp77) is a seven-transmembrane spanning receptor that binds to C5a but lacks motifs essential for G protein coupling and associated signal transduction. expressed on immune cells, modulates various inflammatory diseases in mice, has been shown facilitate murine human regulatory T cell (TREG) generation vitro. Whether how impacts vivo TREG pathogenic cell–dependent disease models have not established. In this article, we show cells express upregulate during induced...
We previously showed that the presence of a CKD-associated locus in SHROOM3 donor kidney results increased expression (an F-actin-binding protein important for epithelial morphogenesis, via rho-kinase [ROCK] binding); this facilitates TGF-b signaling and allograft fibrosis. However, other evidence suggests Shroom3 may have protective role glomerular development.We used human data, knockdown podocytes, inducible shRNA-mediated mice to study adult glomeruli.Expression data from Nephroseq...