A5090771274- Immunodeficiency and Autoimmune Disorders
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Chronic Lymphocytic Leukemia Research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Systemic Sclerosis and Related Diseases
- Platelet Disorders and Treatments
- Gastrointestinal disorders and treatments
- SARS-CoV-2 and COVID-19 Research
- IL-33, ST2, and ILC Pathways
- Immunotherapy and Immune Responses
- Cancer Treatment and Pharmacology
- Chemokine receptors and signaling
- Computational Drug Discovery Methods
- Cell Adhesion Molecules Research
- Blood disorders and treatments
- Retinoids in leukemia and cellular processes
Mount Sinai Medical Center
2024-2025
Icahn School of Medicine at Mount Sinai
2015-2024
Child Health and Development Institute
2024
Institute of Immunology
2015
Hospital Universitario La Paz
2009-2012
Mechanistic target of rapamycin (mTOR) enhances immunity in addition to orchestrating metabolism. Here we show that mTOR coordinates immunometabolic reconfiguration marginal zone (MZ) B cells, a pre-activated lymphocyte subset mounts antibody responses T-cell-independent antigens through Toll-like receptor (TLR)-amplified pathway involving transmembrane activator and CAML interactor (TACI). This interacts with via the TLR adapter MyD88. The resulting activation instigates MZ B-cell...
We previously described that fibroblast-like cells from the synovium of rheumatoid arthritis patients (RASFib) constitutively express intracellular and surface IL-15, which induces activation cocultured T cells. Our objective was to study effect RASFib IL-15 expression on function human CD4(+)CD25(+) regulatory (Treg). RASFib, through their constitutive expression, were able induce proliferation Tregs stimulated TCR, at same time potentiated suppressive action cytokine secretion...
Central B cell tolerance is believed to be regulated by receptor signaling induced the recognition of self-antigens in immature cells. Using humanized mice with defective MyD88, TLR7, or TLR9 expression, we demonstrate that TLR9/MYD88 are required for central and removal developing autoreactive clones. We also show CXCL4, a chemokine involved systemic sclerosis (SSc), abrogates function cells sequestering ligands away from endosomal compartments where this resides. The vivo production CXCL4...
Introduction The purpose of this study was to examine the role RA Synovial Fibroblast (RASFib) IL-15 expression on B cell survival. Methods Magnetically sorted peripheral blood memory cells from 15 healthy subjects were cocultured with RASFib. Results RASFib constitutively expressed membrane IL-15. Survival isolated cultured for 6 days, below 5%, extended in coculture 52+/−8% (p<0.001). neutralizing agents but not isotype controls, reduced rate 31+/−6% (p<0.05). Interestingly, rhIL-15 had no...
TACI signals activate B cell proliferation, isotype switch and antibody production in both normal immunity autoimmune states. In contrast to murine TACI, the human gene undergoes alternative splicing produce short long isoforms (TACI-S TACI-L). previous studies, we showed that transduction of short, but not isoform, into cells or pre-B lacking caused them become transcriptional morphologically identical plasma cells. These data suggest expression different humans provides unique controls on...
WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome is a rare primary immunodeficiency predominantly caused by heterozygous gain-of-function mutations in the C-terminus of gene CXCR4. These CXCR4 variants display impaired receptor trafficking with persistence on surface, resulting hyperactive downstream signaling after CXCL12 stimulation. In turn, this results defective lymphoid differentiation, reduced blood neutrophil lymphocyte numbers. Here, we report mutation...
Distinguishing between primary (PID) and secondary (SID) immunodeficiencies, particularly in relation to hematological B-cell lymphoproliferative disorders (B-CLPD), poses a major clinical challenge. We aimed analyze define the laboratory variables SID patients associated with B-CLPD, identifying overlaps late-onset PIDs, which could potentially improve diagnostic precision prognostic assessment. studied 37 clinical/laboratory 151 B-CLPD. Patients were classified as "Suspected PID Group"...
Abstract Background: Systemic sclerosis (SSc) is an autoimmune connective tissue disease, characterized by defective central B cell tolerance. Deficiency of MYD88, which mediates the function TLRs, results in a failure to silence autoreactive cells. CXCL4, chemokine found elevated sera SSc patients, potentiates IFN-α secretion from TLR9-induced plasmacytoid dendritic The objective investigate whether CXCL4 regulates TLR9 signaling and tolerance Methods: cells patients or healthy donors were...
<title>Abstract</title> Distinguishing between primary (PID) and secondary (SID) immunodeficiencies, particularly in relation to hematological B-cell lymphoproliferative disorders (B-CLPD), poses a major clinical challenge. We aimed analyze define the laboratory variables SID B-CLPD, identifying overlaps with late-onset PIDs, which could potentially improve diagnostic precision prognostic assessment. studied 37 clinical/laboratory 151 patients B-CLPD. Patients were classified as “Suspected...
Abstract The human IgA response is composed of two structurally different subclasses termed 1 and 2 . Compared to , has a shorter hinge region, which makes it more resistant bacterial proteases. produced both systemically in mucosal surfaces, whereas mostly confined the intestines. While overall known be involved intestinal homeostasis, specific contribution remains largely unknown (Chen 2020). Selective deficiency (SIgAD) most prevalent primary immune deficiency. About half SIgAD cases are...