A5044109796- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- SARS-CoV-2 and COVID-19 Research
- Immunotherapy and Immune Responses
- HIV Research and Treatment
- Cancer Immunotherapy and Biomarkers
- COVID-19 Clinical Research Studies
- Eosinophilic Esophagitis
- Clostridium difficile and Clostridium perfringens research
- Inflammatory Bowel Disease
- Cytomegalovirus and herpesvirus research
- Gut microbiota and health
- Monoclonal and Polyclonal Antibodies Research
- IL-33, ST2, and ILC Pathways
- Urinary and Genital Oncology Studies
- Microscopic Colitis
- Biosimilars and Bioanalytical Methods
- Immune Response and Inflammation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immunodeficiency and Autoimmune Disorders
- Bladder and Urothelial Cancer Treatments
- Immune cells in cancer
- Gastrointestinal motility and disorders
- Inflammasome and immune disorders
- Vitamin D Research Studies
Hospital Del Mar
2011-2025
Universitat de Barcelona
2023-2024
Hospital del Mar Research Institute
2013-2023
Icahn School of Medicine at Mount Sinai
2020-2021
Tisch Cancer Institute
2021
Barcelona Biomedical Research Park
2014-2020
Municipal Institute for Medical Research
2015
University of Freiburg
2014
Pompeu Fabra University
2009-2011
Centre de Recherche des Cordeliers
2006-2009
Secretory immunoglobulin A (SIgA) enhances host-microbiota symbiosis, whereas SIgM remains poorly understood. We found that gut IgM+ plasma cells (PCs) were more abundant in humans than mice and clonally related to a large repertoire of memory B disseminated throughout the intestine but rare systemic lymphoid organs. In addition sharing gut-specific gene signature with IgA+ cells, some clonotypes switched IgA response T cell-independent or cell-dependent signals. These signals induced IgM...
The vacuoles, E1-enzyme, X linked, autoinflammatory and somatic (VEXAS) syndrome is an adult-onset disease (AID) due to postzygotic UBA1 variants.To investigate the presence of VEXAS among patients with undiagnosed AID. Additional studies evaluated mosaicism distribution circulating cytokines.Gene analyses were performed by both Sanger amplicon-based deep sequencing. Patients' data collected from their medical charts. Cytokines quantified Luminex.Genetic enrolled (n=42) identified 30...
Abstract Here we report the characterization of 17T2, a SARS-CoV-2 pan-neutralizing human monoclonal antibody isolated from COVID-19 convalescent individual infected during first pandemic wave. 17T2 is class 1 VH1-58/κ3-20 antibody, derived receptor binding domain (RBD)-specific IgA + memory B cell, with broad neutralizing activity against former and new variants, including XBB.1.16 BA.2.86 Omicron subvariants. Consistently, demonstrates in vivo prophylactic therapeutic BA.1.1 infection...
Gain-of-function NLRP3 mutations cause cryopyrin-associated periodic syndrome (CAPS), with gene mosaicism playing a relevant role in the pathogenesis. This study was undertaken to characterize genetic underlying late-onset but otherwise typical CAPS.We studied 64-year-old patient who presented recurrent episodes of urticaria-like rash, fever, conjunctivitis, and oligoarthritis at age 56 years. DNA extracted from both unfractionated blood isolated leukocyte CD34+ subpopulations. Genetic...
Signaling by the B cell–activating receptor TACI drives T cell–independent IgA responses to gut commensal bacteria.
Stromal-derived follicular dendritic cells (FDCs) are essential for germinal centers (GCs), the site where B maturate their antibodies. FDCs present native antigen to and maintain a CXCL13 gradient form cell follicle. Yet despite role, transcriptome of human remains undefined. Using single-cell RNA sequencing microarray, we provided these enigmatic as comprehensive resource. Key genes were validated by flow cytometry microscopy. Surprisingly, marginal reticular (MRCs) rather than expressed...
Apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like 3G (APOBEC3G), a human cytidine deaminase, is potent inhibitor of HIV replication. To explore possible role this protein in modulating vivo susceptibility to infection, we analyzed APOBEC3G expression HIV-exposed seronegative individuals, HIV-seropositive patients, and healthy control subjects. The results showed that the significantly increased peripheral blood mononuclear cells (PBMCs)--mainly CD14(+) cells--and cervical...
Abstract The inhibitory CD94/NKG2A and activating CD94/NKG2C killer lectin-like receptors specific for HLA-E have been reported to be selectively expressed by discrete NK T cell subsets. In the present study, minor proportions of cells coexpressing both were found in fresh peripheral blood from adult donors. Moreover, surface expression was transiently detected upon vitro stimulation CD94/NKG2C+ presence irradiated allogeneic PBMC or rIL-12. A similar effect observed coculture NKG2C+ clones...
ABSTRACT MΦ comprise a heterogeneous population of cells, which contribute to host defense and maintenance immune homeostasis. may be infected by human cytomegalovirus (HCMV), has evolved different strategies subvert the response. In present study, we comparatively analyzed natural killer (NK) cell response against HCMV (TB40E)-infected proinflammatory (M1) antinflammatory (M2) MΦ, derived from autologous monocytes, cultured in presence GM-CSF M-CSF, respectively. M1 were more resistant...
Mechanistic target of rapamycin (mTOR) enhances immunity in addition to orchestrating metabolism. Here we show that mTOR coordinates immunometabolic reconfiguration marginal zone (MZ) B cells, a pre-activated lymphocyte subset mounts antibody responses T-cell-independent antigens through Toll-like receptor (TLR)-amplified pathway involving transmembrane activator and CAML interactor (TACI). This interacts with via the TLR adapter MyD88. The resulting activation instigates MZ B-cell...
The gut microbiota enhances systemic immunoglobulin G (IgG) responses to vaccines, but it is unknown whether this effect involves IgA, which coats intestinal microbes. That IgA may amplify postimmune IgG production suggested by the impaired response pneumococcal vaccines in some IgA-deficient patients. Here, we found that antipneumococcal not total was mice with deficiency. positive of on started very early life and could implicate bacteria, as these were attenuated germ-free recolonized...
mRNA-based vaccines effectively induce protective neutralizing antibodies against SARS-CoV-2, the etiological agent of COVID-19. Yet, kinetics and compositional patterns vaccine-induced antibody responses to original strain emerging variants concern remain largely unknown. Here we characterized serum classes subclasses targeting spike receptor-binding domain SARS-CoV-2 wild type α, β, γ δ in a longitudinal cohort naïve COVID-19 recovered individuals receiving mRNA-1273 vaccine. We found that...