A5015127428- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- COVID-19 epidemiological studies
- Long-Term Effects of COVID-19
- Immunotherapy and Immune Responses
- Animal Virus Infections Studies
- Biosensors and Analytical Detection
- Glycosylation and Glycoproteins Research
- Field-Flow Fractionation Techniques
- Pluripotent Stem Cells Research
- Genetics, Aging, and Longevity in Model Organisms
- CRISPR and Genetic Engineering
- Monoclonal and Polyclonal Antibodies Research
- Advancements in Transdermal Drug Delivery
- Particle Dynamics in Fluid Flows
- vaccines and immunoinformatics approaches
- Innovations in Concrete and Construction Materials
- Neuroinflammation and Neurodegeneration Mechanisms
- Ubiquitin and proteasome pathways
- Toxoplasma gondii Research Studies
- Galectins and Cancer Biology
- Infection Control and Ventilation
- thermodynamics and calorimetric analyses
- Advanced Thermodynamics and Statistical Mechanics
Centre for Genomic Regulation
2020-2024
Barcelona Institute for Global Health
2022
Institute of Science and Technology
2020-2021
Laboratoire Génie de Production
2019
Universitat Politècnica de Catalunya
2019
Center for Research in Agricultural Genomics
2017
Recombinant Antibody Technology (United Kingdom)
2017
Unraveling the long-term kinetics of antibodies to SARS-CoV-2 and individual characteristics influencing it, including impact pre-existing human coronaviruses causing common cold (HCoVs), is essential understand protective immunity COVID-19 devise effective surveillance strategies. IgM, IgA IgG levels against six antigens nucleocapsid antigen four HCoV (229E, NL63, OC43 HKU1) were quantified by Luminex, antibody neutralization capacity was assessed flow cytometry, in a cohort health care...
COVID-19 patients elicit strong responses to the nucleocapsid (N) protein of SARS-CoV-2 but binding antibodies are also detected in prepandemic individuals, indicating potential crossreactivity with common cold human coronaviruses (HCoV) and questioning its utility seroprevalence studies. We investigated immunogenicity full-length shorter fragments N protein, HCoV. identified a C-terminus region SARS-CoV2 minimal sequence homology HCoV that was more specific for highly immunogenic. IgGs...
Reliable serological tests are required to determine the prevalence of antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and characterize immunity disease in order address key knowledge gaps 2019 (COVID-19) pandemic. Quantitative suspension array technology (qSAT) assays based on xMAP Luminex platform overcome limitations rapid diagnostic enzyme-linked immunosorbent (ELISAs) with their higher precision, dynamic range, throughput, miniaturization,...
Two doses of mRNA vaccination have shown >94% efficacy at preventing COVID-19 mostly in naïve adults, but it is not clear if the second dose needed to maximize effectiveness those previously exposed SARS-CoV-2 and what other factors affect responsiveness.We measured IgA, IgG IgM levels against spike (S) nucleocapsid (N) antigens from wild-type S Alpha, Beta Gamma variants concern, after BNT162b2 (Pfizer/BioNTech) or mRNA-1273 (Moderna) a cohort health care workers (N=578). Neutralizing...
Abstract The requirement for Cas nucleases to recognize a specific PAM is major restriction genome editing. SpCas9 variants SpG and SpRY, recognizing NGN NRN PAMs, respectively, have contributed increase the number of editable genomic sites in cell cultures plants. However, their use has not been demonstrated animals. Here we study nuclease activity SpRY by targeting 40 zebrafish C. elegans . Delivered as mRNA-gRNA or ribonucleoprotein (RNP) complexes, were able induce mutations vivo, albeit...
Abstract Background Heterogeneity of the population in relation to infection, COVID-19 vaccination, and host characteristics is likely reflected underlying SARS-CoV-2 antibody responses. Methods We measured IgM, IgA, IgG levels against spike nucleocapsid antigens 1076 adults a cohort study Catalonia between June November 2020 second time May July 2021. Questionnaire data electronic health records on vaccination testing were available both periods. Data several lifestyle, health-related,...
Abstract Here we report the characterization of 17T2, a SARS-CoV-2 pan-neutralizing human monoclonal antibody isolated from COVID-19 convalescent individual infected during first pandemic wave. 17T2 is class 1 VH1-58/κ3-20 antibody, derived receptor binding domain (RBD)-specific IgA + memory B cell, with broad neutralizing activity against former and new variants, including XBB.1.16 BA.2.86 Omicron subvariants. Consistently, demonstrates in vivo prophylactic therapeutic BA.1.1 infection...
We assessed the duration and baseline determinants of antibody responses to SARS-CoV-2 spike antigens occurrence reinfections in a prospective cohort 173 Spanish primary health care worker patients followed initially for 9 months subsequently up 12.5 after COVID-19 symptoms onset. Seropositivity receptor-binding domain 149-270 days was 92.49% (90.17% IgG, 76.3% IgA, 60.69% IgM). In subset 64 workers who had not yet been vaccinated by April 2021, seropositivity 96.88% (95.31% 82.81% IgA)...
SARS-CoV-2 infected pregnant women are at increased risk of severe COVID-19 than non-pregnant and have a higher adverse pregnancy outcomes like intrauterine/fetal distress preterm birth. However, little is known about the impact infection on maternal neonatal immunological profiles. In this study, we investigated inflammatory humoral responses to in cord blood paired samples. Thirty-six were recruited delivery Hospital Sant Joan de Déu, Barcelona, Spain, between April-August 2020, before...
The blood–brain barrier (BBB) is a formidable obstacle for brain delivery of therapeutic antibodies. However, antibodies against the transferrin receptor (TfR), enriched in endothelial cells, have been developed as carriers cargoes into via receptor-mediated transcytosis pathway. In vitro and vivo studies demonstrated that either low-affinity or monovalent binding these to TfR improves their release on abluminal side BBB target engagement parenchyma. performed with mouse-selective recognize...
The development of materials based on renewable and low‐cost resources is today’s crucial interest for civil engineering. This work intends to investigate two new vegetable fibers as composite reinforcement insulation application in the building industry. Miscanthus sunflower stalk have mainly been selected their high fiber content, low water content after harvesting, availability. These criteria lead good structural properties allow a reduction production transportation costs. study aims at...
COVID-19 affects children to a lesser extent than adults but they can still get infected and transmit SARS-CoV-2 their contacts. Field deployable non-invasive sensitive diagnostic techniques are needed evaluate the infectivity dynamics of in pediatric populations guide public health interventions, particularly if this population is not fully vaccinated. We evaluated utility high-throughput Luminex assays quantify saliva IgM, IgA IgG antibodies against five spike (S) nucleocapsid (N) antigens...
The blood-brain barrier (BBB) is a formidable obstacle for delivery of biologic therapeutics to central nervous system (CNS) targets. Whilst the BBB prevents passage vast majority molecules, it also selectively transports wide variety molecules required maintain brain homeostasis. Receptor-mediated transcytosis one example macromolecule transport that employed by cells supply essential proteins and which can be utilized deliver payloads, such as antibodies, across BBB. In this study, we...
ABSTRACT Reliable serological tests are required to determine the prevalence of antibodies against SARS-CoV-2 antigens and characterise immunity disease in order address key knowledge gaps context COVID-19 pandemic. Quantitative suspension array technology (qSAT) assays based on xMAP Luminex platform overcome limitations rapid diagnostic ELISA with their higher precision, dynamic range, throughput, miniaturization, cost-efficacy multiplexing capacity. We developed three qSAT detect IgM, IgA...
Delivery of biologic drugs across the blood-brain barrier is becoming a reality. However, solutions often involve assembly complex multi-specific antibody molecules. Here we utilize simple 12 amino-acid peptide originating from melanotransferrin (MTf) protein that has shown improved brain delivery properties. 3D confocal fluorescence microscopic analysis demonstrated parenchymal localisation fluorescently labelled (NIP228) when chemically conjugated to either MTf or full-length protein....
mRNA-based vaccines effectively induce protective neutralizing antibodies against SARS-CoV-2, the etiological agent of COVID-19. Yet, kinetics and compositional patterns vaccine-induced antibody responses to original strain emerging variants concern remain largely unknown. Here we characterized serum classes subclasses targeting spike receptor-binding domain SARS-CoV-2 wild type α, β, γ δ in a longitudinal cohort naïve COVID-19 recovered individuals receiving mRNA-1273 vaccine. We found that...
Abstract This study evaluated the persistence of IgM, IgA, and IgG to SARS-CoV-2 spike nucleocapsid antigens up 616 days since onset symptoms in a longitudinal cohort 247 primary health care workers from Barcelona, Spain, followed start pandemic. The also assesses factors affecting antibody levels, including comorbidities responses variants concern as well frequency reinfections. Despite gradual significant decline levels with time, seropositivity five combined was always higher than 90%...
Microscale thermophoresis (MST), and the closely related Temperature Related Intensity Change (TRIC), are synonyms for a recently developed measurement technique in field of biophysics to quantify biomolecular interactions, using (capillary-based) NanoTemper Monolith (multiwell plate-based) Dianthus instruments. Although this has been extensively used within scientific community due its low sample consumption, ease use, ubiquitous applicability, MST/TRIC not enjoyed unambiguous acceptance...
Summary The surge of SARS‐CoV‐2 has challenged health systems worldwide and efficient tests to detect viral particles, as well antibodies generated against them, are needed. Specificity, sensitivity, promptness or scalability the main parameters estimate final performance, but rarely all them match in a single test. We have developed SCOVAM, protein microarray with several antigens (spike, nucleocapsid, protease Nsp5) capturing probes fluorescence immunoassay for COVID‐19 serological...
SARS-CoV-2 infection induces virus-reactive memory B cells expressing unmutated antibodies, which hints at their emergence from naïve cells. Yet, the dynamics of virus-specific and impact on immunity immunopathology remain unclear.We longitudinally profiled SARS-CoV-2-specific B-cell responses in 25 moderate-to-severe COVID-19 patients by high-dimensional flow cytometry isotyping subtyping ELISA. We also explored relationship to with activation effector regulatory innate or adaptive immune...
ABSTRACT COVID-19 patients elicit strong responses to the nucleocapsid (N) protein of SARS-CoV-2 but binding antibodies are also detected in prepandemic individuals, indicating potential crossreactivity with common cold human coronaviruses (HCoV) and questioning its utility seroprevalence studies. We investigated immunogenicity full-length shorter fragments N protein, HCoV. indentified a C-terminus region SARS-CoV2 minimal sequence homology HCoV that was more specific highly immunogenic....
Abstract Background Surveillance tools to estimate viral transmission dynamics in young populations are essential guide recommendations for school opening and management during epidemics. Ideally, sensitive techniques required detect low load exposures among asymptomatic children. We aimed SARS-CoV-2 infection rates children adult a school-like environment the initial COVID-19 pandemic waves using an antibody-based field-deployable non-invasive approach. Methods Saliva antibody conversion...
Abstract We evaluated the kinetics of antibody responses to Two years into COVID‐19 pandemic and 1 year after start vaccination rollout, world faced a peak cases associated with highly contagious Omicron variant concern (VoC) severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) spike (S) nucleocapsid (N) antigens over five cross‐sectional visits (January–November 2021), determinants pre‐booster immunoglobulin levels, in prospective cohort vaccinated primary health care workers...
Summary The production of SARS-CoV-2-specific neutralizing antibodies is widely considered as a key mechanism for COVID-19 resolution and protection. However, beyond their protective function, to SARS-CoV-2 may also participate in disease pathogenesis. To explore the potential relationship between virus-specific humoral responses immunopathology, we measured serum antibody classes subclasses receptor-binding domain spike protein nucleoprotein cohort hospitalized patients with moderate severe...