A5040498713- Single-cell and spatial transcriptomics
- Advanced Biosensing Techniques and Applications
- Cell Image Analysis Techniques
- Gene expression and cancer classification
- Cancer Immunotherapy and Biomarkers
- FOXO transcription factor regulation
- Bioinformatics and Genomic Networks
- Software System Performance and Reliability
- Epigenetics and DNA Methylation
- Cancer Cells and Metastasis
- Cancer Genomics and Diagnostics
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- Immunotherapy and Immune Responses
Icahn School of Medicine at Mount Sinai
2019-2021
Rutgers, The State University of New Jersey
2014
Significance A subset of our body’s tissues is continuously renewed through cell division. Tissue-specific stem cells support this tissue turnover, and understanding the mechanisms that control behavior these important to health tissue. In work, we identify a novel regulator intestinal cells. We find that, when transcription factor YY1 inactivated, can no longer renew themselves. show controls mitochondrial gene expression, loss results in structural integrity. This therefore, provides link...
Abstract Mass cytometry is a powerful tool for high‐dimensional single cell characterization. Since the introduction of first commercial CyTOF mass cytometer by DVS Sciences in 2009, technology has matured and become more widely utilized, with sequential platform upgrades designed to address specific limitations expand capabilities platform. Fluidigm's third‐generation Helios introduced number over previous CyTOF2. One these new features modified narrow bore sample injector that generates...
The Cancer Immune Monitoring and Analysis Centers - Immunologic Data Commons (CIMAC-CIDC) Network is supported by the NCI to identify biomarkers of response cancer immunotherapies across clinical trials using state-of-the-art assays. A primary platform for CIMAC-CIDC studies cytometry time flight (CyTOF), performed at all CIMAC laboratories. To ensure ability generate comparable CyTOF data labs, a multistep cross-site harmonization effort was undertaken.
SUMMARY Immunotherapy is becoming a mainstay in the treatment of NSCLC. While tumor mutational burden (TMB) has been shown to correlate with response immunotherapy, little known about relation baseline immune genotype. Here, we profiled 35 early stage NSCLC lesions using multiscale single cell sequencing. Unsupervised clustering identified subset patients key cellular module consisting PDCD1+ CXCL13 + activated T cells, IgG+ plasma and SPP1 macrophages, referred as lung cancer activation...
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<div>AbstractPurpose:<p>The Cancer Immune Monitoring and Analysis Centers – Immunologic Data Commons (CIMAC-CIDC) Network is supported by the NCI to identify biomarkers of response cancer immunotherapies across clinical trials using state-of-the-art assays. A primary platform for CIMAC-CIDC studies cytometry time flight (CyTOF), performed at all CIMAC laboratories. To ensure ability generate comparable CyTOF data labs, a multistep cross-site harmonization effort was...
e15242 Background: The Cancer Immune Monitoring and Analysis Centers – Immunology Data Commons (CIMAC-CIDC) network is a National Institute-funded initiative to identify biomarkers of mechanisms response cancer immunotherapy clinical trials, using state-of-the-art assay technologies. A primary platform for CIMAC-CIDC biomarker studies CyTOF mass cytometry, which performed at all four CIMAC laboratories. Methods: To test the ability generate comparable data across labs, cross-site...
Serial protocols demand high-speed tools and often seem to be beyond the capability of trusted such as logic analysers, which are designed for parallel buses not firehouse serial comms. But with right add on techniques, it is possible get results fast.
Abstract Mass cytometry is a powerful tool for high dimensional single cell characterization. Since the introduction of first commercial CyTOF mass cytometer by DVS Sciences in 2009, technology has matured and become more widely utilized, with sequential platform upgrades designed to address specific limitations expand capabilities platform. Fluidigm’s 3rd-generation Helios introduced number over previous CyTOF2. One these new features modified narrow bore sample injector that generates...