Jessica Le Bérichel
A5052242246- Cancer Immunotherapy and Biomarkers
- Epigenetics and DNA Methylation
- Cancer Research and Treatments
- Immune cells in cancer
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Phagocytosis and Immune Regulation
- Single-cell and spatial transcriptomics
- CAR-T cell therapy research
- Kawasaki Disease and Coronary Complications
- Immune responses and vaccinations
- Pediatric health and respiratory diseases
- Disaster Response and Management
- Histiocytic Disorders and Treatments
- Ferroptosis and cancer prognosis
- Cancer Cells and Metastasis
- COVID-19 Impact on Reproduction
- Diabetes and associated disorders
- Extracellular vesicles in disease
- Long-Term Effects of COVID-19
- COVID-19 epidemiological studies
- Neuroinflammation and Neurodegeneration Mechanisms
- CRISPR and Genetic Engineering
- Intensive Care Unit Cognitive Disorders
- Lung Cancer Research Studies
Icahn School of Medicine at Mount Sinai
2020-2025
Tisch Hospital
2023-2024
Tisch Cancer Institute
2021-2023
Mount Sinai Health System
2021
Immunologie et Neurogénétique Expérimentales et Moléculaires
2016-2018
Université d'Orléans
2016-2018
Centre National de la Recherche Scientifique
2016-2018
University of Cape Town
2016
Abstract It is currently accepted that cancer-associated fibroblasts (CAF) participate in T-cell exclusion from tumor nests. To unbiasedly test this, we used single-cell RNA sequencing coupled with multiplex imaging on a large cohort of lung tumors. We identified four main CAF populations, two which are associated exclusion: (i) MYH11+αSMA+ CAF, present early-stage tumors and form single cell layer lining cancer aggregates, (ii) FAP+αSMA+ appear more advanced organize patches within the...
Age is a major risk factor for cancer, but how aging impacts tumor control remains unclear. In this study, we establish that of the immune system, regardless age stroma and tumor, drives lung cancer progression. Hematopoietic enhances emergency myelopoiesis, resulting in local accumulation myeloid progenitor–like cells tumors. These are source interleukin (IL)–1⍺, which enhanced response. The age-associated decline DNA methyltransferase 3A IL-1⍺ production, disrupting IL-1 receptor 1...
Inflammatory cytokines are fundamental mediators of the organismal response to injury, infection, or other harmful stimuli. To elucidate early and mostly direct transcriptional signatures inflammatory cytokines, we profiled all immunologic cell types by RNAseq after systemic exposure IL1β, IL6, TNFα. Our results revealed a significant overlap in responses, with broad divergence between myeloid lymphoid cells, but very few cell-type-specific responses. Pathway motif analysis identified...
Abstract Post-acute sequelae of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection are debilitating, clinically heterogeneous and unknown molecular etiology. A transcriptome-wide investigation was performed in 165 acutely infected hospitalized individuals who were followed into the post-acute period. Distinct gene expression signatures already present whole blood during infection, with innate adaptive immune cells implicated different symptoms. Two clusters exhibited...
Tissue-resident macrophages (TRMs) populate all tissues and play key roles in homeostasis, immunity repair. TRMs express a molecular program that is mostly shaped by tissue cues. However, TRM identity the mechanisms maintain remain poorly understood. We recently found serous-cavity (LPMs) are highly enriched RXR transcripts RXR-response elements. Here, we show RXRs control mouse serous-macrophage regulating chromatin accessibility transcriptional regulation of canonical macrophage genes....
Multisystem inflammatory syndrome in children (MIS-C) presents with fever, inflammation and pathology of multiple organs individuals under 21 years age the weeks following severe acute respiratory coronavirus 2 (SARS-CoV-2) infection. Although an autoimmune pathogenesis has been proposed, genes, pathways cell types causal to this new disease remain unknown. Here we perform RNA sequencing blood from patients MIS-C controls find disease-associated genes clustered a co-expression module annotated CD56
Abstract Introduction: Chronic inflammation can contribute to growth and immune evasion of solid tumors, with multiple cytokines chemokines implicated in this process. We previously showed that disrupting the Th2 response systemically by blocking IL-4 signaling activate dendritic cells T effector generate a robust against tumor antigens pre-clinical lung cancer models (Maier, B., et al., A conserved dendritic-cell regulatory program limits anti-tumour immunity. Nature,...
Monocyte-derived macrophages (mo-macs) drive immunosuppression in the tumor microenvironment (TME) and tumor-enhanced myelopoiesis bone marrow (BM) fuels these populations. Here, we performed paired transcriptome chromatin analysis over continuum of BM myeloid progenitors, circulating monocytes, tumor-infiltrating mo-macs mice patients with lung cancer to identify progenitor programs that fuel pro-tumorigenic mo-macs. Analyzing accessibility histone mark changes, show tumors prime for Nfe2l2...
Multiplex immunostaining analysis remains fragmented, underperforming, and labor-intensive despite tissue proteomic methodologies achieving ever-increasing marker complexity. Here we propose an open-source, semi-supervised automated pipeline that streamlines start-to-finish, single-cell resolution of whole-slide tissue, named Multiplex-imaging Analysis, Registration, Quantification, Overlaying (MARQO). We compared validated MARQO using Immunohistochemical Consecutive Staining on a Single...
Allergic asthma is characterized by a strong Th2 response with inflammatory cell recruitment and structural changes in the lung. Papain protease allergen disrupting airway epithelium triggering rapid inflammation eosinophilia mediated innate lymphoid activation (ILC2) leading to immune response. In this study, we focused on responses single exposure papain showed that intranasal administration of results cells, including neutrophils eosinophils production IL-1α, IL-1β, IL-33. The abrogated...
Allergic asthma is characterized by a strong Th2 and Th17 response with inflammatory cell recruitment, airways hyperreactivity structural changes in the lung. The protease allergen papain disrupts airway epithelium triggering rapid eosinophilic inflammation innate lymphoid type 2 (ILC2) activation, leading to immune response. Here we asked whether daily oral administrations of probiotic Escherichia coli strain Nissle 1917 (ECN) might affect outcome induced allergic lung BL6 mice. We find...
Abstract Tertiary lymphoid structures (TLS) are organized immune cell aggregates that arise in chronic inflammatory conditions. In cancer, TLS associated with better prognosis and enhanced response to immunotherapy, making these attractive therapeutic targets. However, the mechanisms regulating formation maintenance cancer incompletely understood. Using spatial transcriptomics multiplex imaging across various human tumors, we found an enrichment of mature dendritic cells (DC) expressing high...
Summary We previously found that uptake of cellular debris prompts conventional dendritic cells (cDCs) to undergo maturation. This transformation results in DCs entering the molecular state termed ‘mregDC’. In this state, mregDCs dampen their ability acquire new antigens, upregulate chemokine receptors migrate lymphoid organs, and MHC-I -II, co-stimulatory, -inhibitory molecules promote differentiation antigen-specific T cells. Here, we show cholesterol mobilization – through both de novo...