A5046229204- Immune Cell Function and Interaction
- Complement system in diseases
- T-cell and B-cell Immunology
- Renal Transplantation Outcomes and Treatments
- Immune Response and Inflammation
- Transplantation: Methods and Outcomes
- Chronic Kidney Disease and Diabetes
- Hematopoietic Stem Cell Transplantation
- Renal Diseases and Glomerulopathies
- Acute Kidney Injury Research
- Organ Transplantation Techniques and Outcomes
- COVID-19 Clinical Research Studies
- Receptor Mechanisms and Signaling
- Platelet Disorders and Treatments
- Immunotherapy and Immune Responses
- Extracellular vesicles in disease
- Cell death mechanisms and regulation
- Pancreatic function and diabetes
- Muscle and Compartmental Disorders
- Advanced Glycation End Products research
- COVID-19 Impact on Reproduction
- Mechanical Circulatory Support Devices
- Cardiac Ischemia and Reperfusion
- Lymphatic System and Diseases
- NF-κB Signaling Pathways
Icahn School of Medicine at Mount Sinai
2016-2024
Tisch Hospital
2022
RELX Group (United States)
2017
Alexion Pharmaceuticals (France)
2016
National Institute on Aging
2006
National Institutes of Health
2006
National Cancer Institute
2006
ABSTRACT Importance Preliminary reports indicate that acute kidney injury (AKI) is common in coronavirus disease (COVID)-19 patients and associated with worse outcomes. AKI hospitalized COVID-19 the United States not well-described. Objective To provide information about frequency, outcomes recovery dialysis patients. Design Observational, retrospective study. Setting Admitted to hospital between February 27 April 15, 2020. Participants P atients aged ≥18 years laboratory confirmed Exposures...
Building on studies showing that ischemia-reperfusion-(I/R)-injury is complement dependent, we tested links among activation, transplantation-associated I/R injury, and murine cardiac allograft rejection. We transplanted BALB/c hearts subjected to 8-h cold ischemic storage (CIS) into cytotoxic T-lymphocyte associated protein 4 (CTLA4)Ig-treated wild-type (WT) or c3-/- B6 recipients. Whereas allografts CIS rejected in WT recipients with a median survival time (MST) of 37 days, identically...
CD4+ follicular helper T (Tfh) cells are specialized providers of cell help to B and can function as pathogenic mediators murine antibody-dependent chronic graft-versus-host disease (GvHD). Using a parent→F1 model lupus-like GvHD, in which Tfh germinal center (GC) differentiation occurs over 14 days, we demonstrate that absence cell–expressed C5a receptor 1 (C5ar1) or pharmacological C5aR1 blockade abrogated generation/expansion cells, GC autoantibodies. In cell–dependent GvHD manifested by...
Abstract C5aR2 (C5L2/gp77) is a seven-transmembrane spanning receptor that binds to C5a but lacks motifs essential for G protein coupling and associated signal transduction. expressed on immune cells, modulates various inflammatory diseases in mice, has been shown facilitate murine human regulatory T cell (TREG) generation vitro. Whether how impacts vivo TREG pathogenic cell–dependent disease models have not established. In this article, we show cells express upregulate during induced...
Herein, we report that Shroom3 knockdown, via Fyn inhibition, induced albuminuria with foot process effacement (FPE) without focal segmental glomerulosclerosis (FSGS) or podocytopenia. Interestingly, knockdown mice had reduced podocyte volumes. Human minimal change disease (MCD), where inactivation was reported, also showed lower glomerular volumes than FSGS. We hypothesized volume prevented the progression to To test this hypothesis, utilized unilateral and 5/6th nephrectomy models in...
The pathophysiology of myocardial injury that results from cardiac ischemia and reperfusion (I/R) is incompletely understood. Experimental evidence murine models indicates innate immune mechanisms including complement activation via the classical lectin pathways are crucial. Whether factor B (fB), a component alternative pathway required for amplification cascade activation, participates in I/R has not been addressed. We induced regional by transient coronary ligation WT C57BL/6 mice,...
SHARPIN, together with RNF31/HOIP and RBCK1/HOIL1, form the linear ubiquitin chain assembly complex (LUBAC) E3 ligase that catalyzes M1-linked polyubiquitination. Mutations in
TNF ligation of receptor 1 (TNFR1) promotes either inflammation and cell survival by (a) inhibiting RIPK1's death-signaling function activating NF-κB or (b) causing RIPK1 to associate with the death-inducing signaling complex initiate apoptosis necroptosis. The cellular source that results in RIPK1-dependent death remains unclear. To address this, we employed vitro systems murine models T cell–dependent transplant tumor rejection which target susceptibility could be genetically altered. We...
Natural Killer (NK) cells respond to diseased and allogeneic through NKG2A/HLA-E or Killer-cell Immunoglobulin-like receptor (KIR)/HLA-ABC interactions. Correlations between HLA/KIR disparities kidney transplant pathology suggest an antibody-independent pathogenic role for NK in transplantation, but mechanisms remain unclear. Using CyTOF characterize recipient peripheral cell phenotypes function, we observed diverse subsets amongst participants that responded heterogeneously...
ABSTRACT Human Natural Killer (NK) cells are heterogeneous lymphocytes regulated by variegated arrays of germline-encoded activating and inhibitory receptors. They acquire the ability to detect polymorphic self-antigen via NKG2A/HLA-E or KIR/HLA-I ligand interactions through an education process. Correlations among HLA/KIR genes, kidney transplantation pathology outcomes suggest that NK participate in allograft injury, but mechanisms linking antibody-independent pathological functions remain...
Conditioning regimens used for hematopoietic stem cell transplantation (HCT) can escalate the severity of acute T cell-mediated graft-versus-host disease (GVHD) by disrupting gastrointestinal integrity and initiating lipopolysaccharide (LPS)-dependent innate immune activation. Activation complement cascade has been associated with murine GVHD, previous work shown that alternative pathway activation amplify immunity. Whether how mannan-binding lectin (MBL), a component system binds mannose as...
Abstract We reported that Shroom3 knockdown, via Fyn inhibition, induced albuminuria with foot process effacement (FPE) without glomerulosclerosis (FSGS) or podocytopenia. Interestingly, knockdown mice had reduced podocyte volumes. Human minimal change disease, where inactivation was reported, also showed lower glomerular volumes than FSGS. hypothesized volume prevented the progression to To test this hypothesis, we utilized unilateral- and 5/6 th nephrectomy models in mice. Knockdown...