Brian H. Lee
A5101641626- T-cell and B-cell Immunology
- Multiple Myeloma Research and Treatments
- Chemokine receptors and signaling
- CAR-T cell therapy research
- Lymphoma Diagnosis and Treatment
- Blood disorders and treatments
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- Chronic Lymphocytic Leukemia Research
- Immunodeficiency and Autoimmune Disorders
- RNA and protein synthesis mechanisms
- CNS Lymphoma Diagnosis and Treatment
- Renal Transplantation Outcomes and Treatments
- SARS-CoV-2 and COVID-19 Research
- Enzyme Structure and Function
- Long-Term Effects of COVID-19
- Extracellular vesicles in disease
- Protein Structure and Dynamics
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Cell Adhesion Molecules Research
- Acute Myeloid Leukemia Research
- Single-cell and spatial transcriptomics
- COVID-19 Clinical Research Studies
City of Hope
2006-2024
City Of Hope National Medical Center
2024
Icahn School of Medicine at Mount Sinai
2019-2023
Nationwide Children's Hospital
2022
The IMPACC cohort, composed of >1,000 hospitalized COVID-19 participants, contains five illness trajectory groups (TGs) during acute infection (first 28 days), ranging from milder (TG1–3) to more severe disease course (TG4) and death (TG5). Here, we report deep immunophenotyping, profiling >15,000 longitudinal blood nasal samples 540 participants the using 14 distinct assays. These unbiased analyses identify cellular molecular signatures present within 72 h hospital admission that...
BACKGROUND.Common variable immunodeficiency (CVID) is the most common symptomatic primary and frequently complicated by interstitial lung disease (ILD) for which etiology unknown therapy inadequate. METHODS.Medical record review implicated B cell dysregulation in CVID ILD progression.This was further studied blood samples using culture, cytometry, ELISA, histology.Eleven patients were treated with rituximab followed 18 months. RESULTS.Serum IgM increased conjunction progression, a finding...
As part of the Multiple Myeloma Research Foundation (MMRF) immune atlas pilot project, we compared cells multiple myeloma bone marrow samples from 18 patients assessed by single-cell RNA sequencing (scRNA-seq), mass cytometry (CyTOF), and cellular indexing transcriptomes epitopes (CITE-seq) to understand concordance measurements among techniques. Cell type abundances are relatively consistent across three approaches, while variations observed in T cells, macrophages, monocytes. Concordance...
Chimeric antigen receptor T-cell therapy (CAR-T) has altered the treatment landscape of several hematologic malignancies. Until recently, most CAR-T infusions have been administered in inpatient setting, due to their toxicity profile. However, advent new product constructs, as well improved detection and management adverse effects, greatly increased safety administering these therapies. indications continue expand, administration is associated with healthcare resource utilization overall...
ABSTRACT Human Natural Killer (NK) cells are heterogeneous lymphocytes regulated by variegated arrays of germline-encoded activating and inhibitory receptors. They acquire the ability to detect polymorphic self-antigen via NKG2A/HLA-E or KIR/HLA-I ligand interactions through an education process. Correlations among HLA/KIR genes, kidney transplantation pathology outcomes suggest that NK participate in allograft injury, but mechanisms linking antibody-independent pathological functions remain...
<div><p>As part of the Multiple Myeloma Research Foundation (MMRF) immune atlas pilot project, we compared cells multiple myeloma bone marrow samples from 18 patients assessed by single-cell RNA sequencing (scRNA-seq), mass cytometry (CyTOF), and cellular indexing transcriptomes epitopes (CITE-seq) to understand concordance measurements among techniques. Cell type abundances are relatively consistent across three approaches, while variations observed in T cells, macrophages,...
<div><p>As part of the Multiple Myeloma Research Foundation (MMRF) immune atlas pilot project, we compared cells multiple myeloma bone marrow samples from 18 patients assessed by single-cell RNA sequencing (scRNA-seq), mass cytometry (CyTOF), and cellular indexing transcriptomes epitopes (CITE-seq) to understand concordance measurements among techniques. Cell type abundances are relatively consistent across three approaches, while variations observed in T cells, macrophages,...
<p>Expression of cell type markers in CITE-seq and comparison subset abundance between technical replicates across different centers</p>