A5088980123- Renal Transplantation Outcomes and Treatments
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Complement system in diseases
- Immunotherapy and Immune Responses
- Transplantation: Methods and Outcomes
- Organ Transplantation Techniques and Outcomes
- Cytomegalovirus and herpesvirus research
- Renal Diseases and Glomerulopathies
- Immune Response and Inflammation
- Viral Infections and Immunology Research
- Xenotransplantation and immune response
- Systemic Lupus Erythematosus Research
- Hepatitis C virus research
- Blood groups and transfusion
- COVID-19 Clinical Research Studies
- Adenosine and Purinergic Signaling
- Hematopoietic Stem Cell Transplantation
- Diabetes and associated disorders
- Advanced biosensing and bioanalysis techniques
- SARS-CoV-2 and COVID-19 Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Erythropoietin and Anemia Treatment
- Neurological Complications and Syndromes
- Liver Disease and Transplantation
Cedars-Sinai Medical Center
2022-2025
Sinai Hospital
2013-2025
Icahn School of Medicine at Mount Sinai
2015-2024
Mount Sinai Hospital
2022
Tisch Hospital
2011-2022
Emory University
2021
Johns Hopkins Medicine
2021
Johns Hopkins University
2021
Mount Sinai Health System
2019
RELX Group (United States)
2017
While studies in animal models have linked Toll-like receptor (TLR) 4 signaling to kidney injury induced by ischemia and reperfusion, the relevance of TLR4 activation allograft human transplants is unknown. Here we show that constitutively expressed within all donor kidneys but significantly higher deceased-, compared with living-donor organs. Tubules from deceased- not also stained positively for high-mobility group box-1 (HMGB1), a known endogenous ligand. In vitro stimulation tubular...
The standard test for the diagnosis of acute rejection in kidney transplants is renal biopsy. Noninvasive tests would be preferable.
Noninvasive biomarkers are needed to assess immune risk and ultimately guide therapeutic decision-making following kidney transplantation. A requisite step toward these goals is validation of markers that diagnose and/or predict relevant transplant endpoints. The Clinical Trials in Organ Transplantation-01 protocol a multicenter observational study 280 adult pediatric first recipients. We compared validated urinary mRNAs proteins as biopsy-proven acute rejection (AR) stratify patients into...
Abstract While matching for MHC Ags improves renal allograft survival, closely matched grafts sometimes fail due to rejection, and poorly allografts are often well tolerated by the recipient. The severity of rejection process may partially depend on presence environmentally primed T cells in recipient that cross-react with donor Ags. To test primed, donor-specific humans before transplantation, we used an enzyme-linked immunospot assay detection allospecific cytokines produced individual...
Abstract The immune response to transplanted allogeneic tissues is mediated by T cells that recognize donor histocompatibility Ags either via direct (donor MHC and peptides) or indirect (recipient donor-derived allorecognition pathways. relative contribution of each these pathways allograft rejection remains largely unknown. To address this, we used an enzyme-linked immunospot assay define the frequency cytokine phenotype responding alloantigens at various time points following placement...
Decay-accelerating factor (Daf) dissociates C3/C5 convertases that assemble on host cells and thereby prevents complement activation their surfaces. We demonstrate during primary T cell activation, the absence of Daf antigen-presenting (APCs) enhances proliferation augments induced frequency effector cells. The effect is D- and, at least in part, C5-dependent, indicating local alternative pathway essential. show cognate cell–APC interactions are accompanied by rapid production components...
A charge neutral complex (CNC) was formed in aqueous solution by combining an orange light emitting anionic conjugated polyelectrolyte and a saturated cationic at 1:1 ratio (per repeat unit). Photoluminescence (PL) from the CNC can be quenched both negatively charged dinitrophenol (DNP) derivative, (DNP-BS − ), positively methyl viologen (MV 2+ ). Use of minimizes nonspecific interactions (which modify PL) between polyelectrolytes biopolymers. Quenching PL DNP derivative specific unquenching...
Traditionally, protein Ags have been injected in CFA (oil with inactivated mycobacteria) to induce immunity and IFA alone) tolerance. We report here that injection of hen eggwhite lysozyme, a prototypic Ag, CFA-induced IFA-induced pools lysozyme-specific memory T cells comparable fine specificity, clonal size, avidity spectrum, but type-1 type-2 cytokine signatures, respectively. This adjuvant-guided induction virtually unipolar was observed seven total six mouse strains. Highly polarized...
Costimulatory blockade can induce long‐term allograft survival in naïve animals, but may not be as effective animals with previously primed immune repertoires. We attempted to graft B10.D2 recipients of B10.A cardiac allografts using donor‐specific transfusion (DST) plus anti‐CD40 ligand antibody (αCD40L). Recipients were either mice, or mice third party alloantigens through engraftment and rejection skin transplants. Untreated rejected transplants by day 15 contained a high frequency...
Thymus-derived (natural) CD4+ FoxP3+ regulatory T cells (nT reg cells) are required for immune homeostasis and self-tolerance, but must be stringently controlled to permit expansion of protective immunity. Previous findings linking signals transmitted through cell–expressed C5a receptor (C5aR) C3a (C3aR) activation, differentiation, conventional CD4+CD25− (T conv cells), raised the possibility that C3aR/C5aR signaling on nT could physiologically modulate cell function thereby further impact...
Concerns about adverse effects of calcineurin inhibitors (CNIs) have prompted development protocols that minimize their use. Whereas previous CNI withdrawal trials in heterogeneous cohorts showed unacceptable rates acute rejection (AR), we hypothesized could identify individuals capable tolerating by targeting immunologically quiescent kidney transplant recipients. The Clinical Trials Organ Transplantation-09 Trial was a randomized, prospective study nonsensitized primary recipients living...
Acute graft-versus-host disease (GVHD) results from the attack of host tissues by donor allogeneic T cells and is most serious limitation hematopoietic cell transplantation (allo-HCT). Host antigen-presenting are thought to control priming alloreactive induction acute GVHD after allo-HCT. However, whereas role DC in has been established, contribution macrophages not clearly addressed. We show that, contrast DC, reducing macrophage pool recipient mice increased expansion aggravated mortality...