A5074841528- Renal Diseases and Glomerulopathies
- Renal Transplantation Outcomes and Treatments
- Organ Transplantation Techniques and Outcomes
- Polyomavirus and related diseases
- Full-Duplex Wireless Communications
- Chronic Lymphocytic Leukemia Research
- Complement system in diseases
- Transplantation: Methods and Outcomes
- Autoimmune Bullous Skin Diseases
- Tuberous Sclerosis Complex Research
- Venous Thromboembolism Diagnosis and Management
- Vasculitis and related conditions
- T-cell and B-cell Immunology
- Energy Harvesting in Wireless Networks
- Skin Diseases and Diabetes
- Cardiac Valve Diseases and Treatments
- Pregnancy and Medication Impact
- Reproductive System and Pregnancy
- Cytomegalovirus and herpesvirus research
- Inflammatory Myopathies and Dermatomyositis
- Dermatological and Skeletal Disorders
- Cell death mechanisms and regulation
- Mesenchymal stem cell research
- Genomic variations and chromosomal abnormalities
- Folate and B Vitamins Research
Istituto Giannina Gaslini
2012-2024
Istituti di Ricovero e Cura a Carattere Scientifico
2010-2022
Children's Research Hospital
2021
University of Genoa
2015-2019
University of Basel
2013-2015
San Raffaele University of Rome
2010-2014
Vita-Salute San Raffaele University
2012
University of Pavia
2007-2010
ANT Foundation Italy Onlus
2006
University of Bari Aldo Moro
2006
Polyoma BK virus (BKV)-associated nephropathy (PVAN) is a relevant cause of poor renal allograft survival. In prospective analysis, we monitored BKV DNA in blood and urine samples from 62 consecutive pediatric kidney recipients. patients with replication, analyzed the impact reduction maintenance immunosuppression on viral load kinetics PVAN replication. BKV-specific immunity was concomitantly evaluated viremic patients, by measuring frequency interferon-gamma-producing cytotoxic T cells,...
The emerging role of humoral immunity in the pathogenesis chronic allograft damage has prompted research aimed at assessing anti-HLA antibody (Ab) monitoring as a tool to predict outcome. Data on natural history allografts children developing de novo Ab after transplantation are limited. Utilizing sera collected pretransplant, and serially posttransplant, we retrospectively evaluated 82 consecutive primary pediatric kidney recipients, without pretransplant donor-specific antibodies (DSA),...
Background. Antibodies directed against alloantigens are implicated in the pathogenesis of several immune reactions complicating transplantation, including humoral rejection after solid organ transplantation. Mesenchymal stem cells (MSCs) have immunomodulatory capacity, since vivo they may prolong skin graft survival animal model and can rescue patients with life-threatening graft- versus -host disease. Methods. To investigate whether MSCs exert an inhibitory effect on antibody production...
Alloantibody-mediated graft injury is a major cause of kidney dysfunction and loss. The complement-binding ability de novo donor-specific antibodies (dnDSAs) has been suggested as prognostic tool to stratify patients for clinical risk. In this study, we analyzed posttransplant kinetics complement-fixing dnDSAs their role in antibody-mediated rejection development A total 114 pediatric nonsensitized recipients first allograft were periodically monitored using flow bead assays, followed by C3d...
Bortezomib is a selective and reversible inhibitor of the 26S proteasome that shows potent antitumor activity in vitro vivo against several human cancers adulthood. No data are available on bortezomib pediatric neuroblastoma.Ten neuroblastoma cell lines suspensions primary cells from three patients were tested for sensitivity to bortezomib. Colony formation, proliferation, cycle progression, apoptosis evaluated by clonogenic assay measuring 3H-thymidine incorporation, bromodeoxyuridine...
Significance Statement In patients with steroid-dependent and calcineurin inhibitor–depe ndent nephrotic syndrome, rituximab, a chimeric monoclonal anti body directed against CD20+ B cells, helps maintain remission, but relapse within year is common. This randomized trial investigated wheth er ofatumumab, fully human anti-CD20 antibody, superior to rituximab in maintaining oral drug–free remission this condition. The findings show ofatumumab not achieving at 1 of follow-up, had similar...
Computed tomography (CT) is an increasingly utilized method for the evaluation of patient suitability transcatheter aortic valve implantation (TAVI). The aim this study was to analyse role CT in choice prosthesis and prevention residual regurgitation (RAR).From November 2007 September 2010, 115 patients (median age 81 years, inter-quantile range (IQR) 76-85; median ejection fraction 55%, IQR 45-60; logistic EuroSCORE 19.7, 11.0-32.1) undergoing TAVI were evaluated with a pre-procedural CT....
Uncontrolled BK polyomavirus (BKPyV) replication in kidney transplant recipients (KTRs) causes polyomavirus-associated nephropathy and allograft loss. Reducing immunosuppression is associated with clearing viremia increasing BKPyV-specific T cell responses most patients; however, current immunoassays have limited sensitivity, target mostly CD4(+) cells, largely fail to predict onset clearance of BKPyV replication. To characterize CD8(+) bioinformatics were used 9mer epitopes the early viral...
Anti-CD20 antibodies are increasingly being used to treat idiopathic nephrotic syndrome (INS) in children. While they may allow steroid and calcineurin inhibitor withdrawal, repeated infusions of anti-CD20 often required maintain remission. Data on their potential toxicity INS needed, consider infusions.
Abstract Background Steroid resistant nephrotic syndrome (SRNS) is a frequent cause of end stage renal disease in children and post-transplant recurrence major graft loss. Methods We identified all with SRNS who underwent transplantation Italy, between 2005 2017. Data were retrospectively collected for the presence causative gene mutation, sex, histology, duration pre-transplant dialysis, age at onset transplant, HLA matching, recurrence, therapy survival. Results 101 patients first 22...
B cell depleting therapies permit immunosuppressive drug withdrawal and maintain remission in patients with frequently relapsing nephrotic syndrome (FRNS) or steroid–dependent (SDNS), but lack of biomarkers for treatment failure. Post-depletion immune reconstitution may identify patients, previous characterizations suffered from methodological limitations flow cytometry. Time-of-flight mass cytometry (CyTOF) is a comprehensive analytic modality that simultaneously quantifies over 40 cellular...
Summary The mechanism responsible for proteinuria in non-genetic idiopathic nephrotic syndrome (iNS) is unknown. Animal models suggest an effect of free radicals on podocytes, and indirect evidence humans confirm this implication. We determined the oxidative burst by blood CD15+ polymorphonucleates (PMN) utilizing 5-(and-6)-carboxy-2′,7′-dichlorofluorescin diacetate (DCF-DA) fluorescence assay 38 children with iNS. Results were compared PMN from normal subjects patients renal pathologies...
Immunosuppressive regulatory T cells (Tregs) have been hypothesized to exert a protective role in animal models of spontaneous (Buffalo/Mna) and/or drug induced (Adriamycin) nephrotic syndrome. In this study, we thought define whether Tregs can modify the outcome LPS nephropathy utilizing IL-2 as inducer tissue and circulating Tregs. (12 mg/Kg) was given single shot C57BL/6, p2rx7−/− Foxp3EGFP; free (18.000 U) or, alternative, coupled with JES6-1 mAb (IL-2/anti-IL-2) were injected before...
Donor-specific HLA antibody (DSA)-mediated graft injury is the major cause of kidney loss. Among DSA characteristics, homing has been suggested as an indicator severe tissue damage. We analyzed role de novo (dnDSA) on transplantation outcome. Graft biopsy specimens and parallel sera from 48 nonsensitized pediatric recipients were analyzed. Serum samples eluates tested for presence dnDSAs with flow bead technology. Intragraft (gDSAs) never detected in absence serum (sDSAs), whereas sDSAs,...
Abstract Background Scleromyxoedema is a primary fibro‐mucinosis whose therapy still challenging. Objective To evaluate the safety and efficacy of high‐dose intravenous immunoglobulin ( IVI g) for management scleromyxoedema prospectively using an objective score. Methods In prospective open‐label study, g was administered to eight patients with in dose 2 g/kg per month. The were followed‐up minimum 6 months, their disease activity response treatment assessed Physician's Global Assessment...
De novo posttransplant donor-specific HLA-antibody ( dn DSA) detection is now recognized as a tool to identify patients at risk for antibody-mediated rejection (AMR) and graft loss. It still unclear whether the time interval from transplant DSA occurrence influences damage. Utilizing sera collected longitudinally, we evaluated 114 consecutive primary pediatric kidney recipients grafted between 2002 2013 by Luminex platform. DSAs occurred in 39 median of 24.6 months. In 15 patients, developed...
Vascular and bleeding complications remain important in patients undergoing percutaneous transfemoral transcatheter aortic valve implantation (TF-TAVI). Platelets play an role events. Mean platelet volume (MPV) is indicator of activation. The objective this study was to assess whether low MPV major vascular following TF-TAVI.A retrospective cohort 330 subjects TF-TAVI performed. primary endpoint the occurrence combined safety (CSEP); secondary endpoints included life-threatening bleeding....
Development of de novo donor-specific antibodies (dnDSA) is associated with late or chronic antibody-mediated rejection (CAMR) and poor graft outcome in low-risk kidney transplant recipients. High-level soluble B-cell activating factor (sBAFF) was observed recipients at higher risk developing dnDSA.We longitudinally analyzed sBAFF levels 81 consecutive primary pediatric monitored for human leukocyte antigen (HLA) antibody (Ab) occurrence to gain insight into the events conditioning...
Background . Among human polyomaviruses, only BK virus (BKV) and JC (JCV) encode an agnoprotein upstream of VP1 on the viral late transcript. BKV is abundantly expressed in life cycle, but specific cellular humoral immune responses are low or absent. We hypothesized that might contribute to evasion by downregulating HLA expression, similar Herpes simplex virus-1 ICP47. Methods UTA-6 primary renal proximal tubular epithelial cells (RPTEC) were co-transfected with plasmids constitutively...