A5008560276- Renal Transplantation Outcomes and Treatments
- Organ Transplantation Techniques and Outcomes
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Cytomegalovirus and herpesvirus research
- Organ Donation and Transplantation
- Renal Diseases and Glomerulopathies
- Polyomavirus and related diseases
- Diabetes and associated disorders
- Complement system in diseases
- Transplantation: Methods and Outcomes
- Blood groups and transfusion
- Pancreatic function and diabetes
- Viral-associated cancers and disorders
- Monoclonal and Polyclonal Antibodies Research
- Parvovirus B19 Infection Studies
- Pregnancy and Medication Impact
- Immunodeficiency and Autoimmune Disorders
- Hematopoietic Stem Cell Transplantation
- Acute Lymphoblastic Leukemia research
- Xenotransplantation and immune response
- Pancreatitis Pathology and Treatment
- Pediatric Urology and Nephrology Studies
- Full-Duplex Wireless Communications
- Renin-Angiotensin System Studies
Istituti di Ricovero e Cura a Carattere Scientifico
1985-2024
Istituto Giannina Gaslini
2002-2024
Ospedale Policlinico San Martino
2000-2017
University of Genoa
1993-2016
University of Palermo
2016
University of Milan
1998-2005
Azienda Ospedaliera S.Maria
2004
University of Florence
2003
Versiti Blood Center of Wisconsin
1993-1999
Fondazione Salvatore Maugeri
1999
Background. Although a growing body of literature regarding polyoma BK virus (BKV) infection and associated interstitial nephritis in kidney-allograft recipients is becoming available, the impact BKV pediatric population has not been fully evaluated. Methods. In retrospective analysis, we performed polymerase chain reaction (PCR) assays for DNA serum urine samples from 100 referred to our institution last 5 years. Results. viruria was observed 26 patients, whereas viremia demonstrated...
The emerging role of humoral immunity in the pathogenesis chronic allograft damage has prompted research aimed at assessing anti-HLA antibody (Ab) monitoring as a tool to predict outcome. Data on natural history allografts children developing de novo Ab after transplantation are limited. Utilizing sera collected pretransplant, and serially posttransplant, we retrospectively evaluated 82 consecutive primary pediatric kidney recipients, without pretransplant donor-specific antibodies (DSA),...
Alloantibody-mediated graft injury is a major cause of kidney dysfunction and loss. The complement-binding ability de novo donor-specific antibodies (dnDSAs) has been suggested as prognostic tool to stratify patients for clinical risk. In this study, we analyzed posttransplant kinetics complement-fixing dnDSAs their role in antibody-mediated rejection development A total 114 pediatric nonsensitized recipients first allograft were periodically monitored using flow bead assays, followed by C3d...
The treatment of EBV-associated post-transplant lymphoproliferative disease (PTLD) poses a considerable challenge. Efforts have been made to define regimens based on combination the available therapeutic agents, chosen and tailored patient-by-patient basis, with aim augmenting event-free patient graft survival. Recently, autologous EBV-specific cytotoxic T-lymphocytes (CTL) proved effective in enhancing immune responses reducing viral load organ transplant recipients active infection. We...
ABSTRACT. Polyoma BK virus (BKV)-associated interstitial nephritis has emerged as a relevant complication of immunocompromise after kidney transplantation, leading to reduced survival the renal allograft. The limitations current antiviral treatment and high probability rejection in graft recipients when control viral replication is attempted by reduction immunosuppression warrant further efforts develop alternative therapeutic tools. Cellular immunotherapy proved be successful approach for...
Abstract: From June 1985 to December 1998, 173 pediatric renal transplants were carried out in 170 patients at our center. this pool, 73 (34 males and 39 females) with a follow‐up of 48 months examined. In all patients, ureteroneocystostomy was performed according the Lich‐Grégoire procedure. All treated cyclosporin A (CsA)‐based immunosuppression, including prednisone sometimes azathioprine (AZA). Six after transplantation, voiding cystography (VCU) reflux classified from Grade I IV. The...
Classical and nonclassical MHC class II (MHCII) genes are coregulated by the transcription factor RFX (regulatory X) transcriptional coactivator CIITA. coordinates assembly of a multiprotein "enhanceosome" complex on MHCII promoters. This enhanceosome serves as docking site for binding Whereas role in recruiting CIITA is well established, little known about its CIITA-independent functions. A novel was revealed analysis HLA-DOA expression human MHCII-negative B cell lines lacking or found to...
De novo posttransplant donor-specific HLA-antibody ( dn DSA) detection is now recognized as a tool to identify patients at risk for antibody-mediated rejection (AMR) and graft loss. It still unclear whether the time interval from transplant DSA occurrence influences damage. Utilizing sera collected longitudinally, we evaluated 114 consecutive primary pediatric kidney recipients grafted between 2002 2013 by Luminex platform. DSAs occurred in 39 median of 24.6 months. In 15 patients, developed...
Despite numerous advances in the areas of organ preservation, histocompatibility, and immunosuppression, chronic deterioration allograft function, referred to as "chronic rejection," still remains main obstacle long-term graft survival. The common feature rejection is a concentric generalized arteriosclerosis associated with interstitial fibrosis that reflects an allogeneic injury arteries, possibly worsened by other alloantigen-independent risk factors. presence angiotensin I-converting...
Abstract: Although both immunologic and non‐immunologic components may cause kidney allograft chronic rejection (KGCR), also referred to as nephropathy (CAN), its pathogenesis is largely not yet understood. To explore relevant mechanisms occurring in KGCR, we have analyzed surgically removed KG the transcription of following cytokine apoptotic molecule genes: interleukin (IL)‐2, IL‐3, IL‐4, IL‐5, IL‐6, IL‐10, tumor necrosis factor (TNF)‐ α , IFN‐ γ FAS, FAS‐L. Semiquantitative RT‐PCR was...
The objective of this study was to evaluate the specificities HLA class I (-A,-B) and II (-DR,-DQ) antibodies (Ab) detected in sera alloimmunized patients waiting for a subsequent renal transplantation. group consisted 62 dialysis (42 men 20 women, mean age: 43 +/- 18 yr) on list kidney transplant (52 second 10 third transplant) at S. Martino Hospital Transplant Centre Genoa/Italy, who were enrolled from 2002 2004 antibody screening. Complement dependent cytotoxicity (CDC) technique used...