A5043282361- Amyotrophic Lateral Sclerosis Research
- Neurogenetic and Muscular Disorders Research
- Pluripotent Stem Cells Research
- Polymer Nanocomposites and Properties
- Polyomavirus and related diseases
- RNA Research and Splicing
- Genetic Neurodegenerative Diseases
- Hippo pathway signaling and YAP/TAZ
- Full-Duplex Wireless Communications
- SARS-CoV-2 and COVID-19 Research
- Molecular Communication and Nanonetworks
- Polymer crystallization and properties
- Parvovirus B19 Infection Studies
- Retinal Imaging and Analysis
- Light effects on plants
- Wireless Body Area Networks
- Natural Fiber Reinforced Composites
- Reproductive Biology and Fertility
- Epoxy Resin Curing Processes
- Energy Harvesting in Wireless Networks
- Neuroscience and Neural Engineering
- Bone Tissue Engineering Materials
- Planarian Biology and Electrostimulation
- Muscle Physiology and Disorders
- Morphological variations and asymmetry
IRCCS Eugenio Medea
2018-2024
Rockefeller University
2019-2023
University of Padua
2023
Sapienza University of Rome
2015-2021
Italian Institute of Technology
2015-2021
University of Brescia
2009-2012
University of Florence
1996-2012
National Research Council
2012
Ospedale Policlinico San Martino
2004
Mario Negri Institute for Pharmacological Research
2004
Abstract The RNA-binding protein FUS participates in several RNA biosynthetic processes and has been linked to the pathogenesis of amyotrophic lateral sclerosis (ALS) frontotemporal dementia. Here we report that controls back-splicing reactions leading circular (circRNA) production. We identified circRNAs expressed vitro -derived mouse motor neurons (MNs) determined production a considerable number these is regulated by FUS. Using RNAi overexpression wild-type ALS-associated mutants,...
Background. Although a growing body of literature regarding polyoma BK virus (BKV) infection and associated interstitial nephritis in kidney-allograft recipients is becoming available, the impact BKV pediatric population has not been fully evaluated. Methods. In retrospective analysis, we performed polymerase chain reaction (PCR) assays for DNA serum urine samples from 100 referred to our institution last 5 years. Results. viruria was observed 26 patients, whereas viremia demonstrated...
Patient-derived induced Pluripotent Stem Cells (iPSCs) provide an opportunity to study human diseases mainly in those cases where no suitable model systems are available. Here we have taken advantage of vitro iPSCs derived from patients affected by Amyotrophic Lateral Sclerosis and carrying mutations the RNA-binding proteins FUS cellular behavior mutant appropriate genetic background. Moreover, ability differentiate into spinal cord neural cells provides mimicking physiological conditions....
The FUS gene has been linked to amyotrophic lateral sclerosis (ALS). is a ubiquitous RNA-binding protein, and the mechanisms leading selective motoneuron loss downstream of ALS-linked mutations are largely unknown. We report transcriptome analysis human purified motoneurons, obtained from wild-type or mutant isogenic induced pluripotent stem cells (iPSCs). Gene ontology differentially expressed genes identified significant enrichment pathways previously associated sporadic ALS other...
Human pluripotent stem cells (PSCs) are widely used for in vitro disease modeling. One of the challenges field is represented by ability converting human PSCs into specific disease-relevant cell types. The nervous system composed a wide variety neuronal types with selective vulnerability neurodegenerative diseases. This particularly relevant motor neuron diseases, which different neurons populations show susceptibility to degeneration. Here we developed fast and efficient method convert...
Abstract Organizing centers secrete morphogens that specify the emergence of germ layers and establishment body’s axes during embryogenesis. While traditional experimental embryology tools have been instrumental in dissecting molecular aspects organizers model systems, they are impractical human in-vitro systems to dissect relationships between signaling fate along embryonic coordinates. To systematically study organizer centers, we devised a collection optogenetic ePiggyBac vectors express...
ABSTRACT. Polyoma BK virus (BKV)-associated interstitial nephritis has emerged as a relevant complication of immunocompromise after kidney transplantation, leading to reduced survival the renal allograft. The limitations current antiviral treatment and high probability rejection in graft recipients when control viral replication is attempted by reduction immunosuppression warrant further efforts develop alternative therapeutic tools. Cellular immunotherapy proved be successful approach for...
Amyotrophic Lateral Sclerosis (ALS) is a severe and fatal neurodegenerative disease characterized by progressive loss of motoneurons, muscle atrophy paralysis. Recent evidence suggests that ALS should be considered as multi-systemic disease, in which several cell types contribute to motoneuron degeneration. In this view, mutations linked genes other neural non-neural may exert non-cell autonomous effects on survival function. Induced Pluripotent Stem Cells (iPSCs) have been recently derived...
While studied extensively in model systems, human gastrulation remains obscure. The scarcity of fetal biological material as well ethical considerations limit our understanding this process. In vitro attachment natural blastocysts shed light on aspects the second week development absence morphological manifestation gastrulation. Stem cell-derived blastocyst models, blastoids, provide opportunity to reconstitute pre- post-implantation vitro. Here we show that upon attachment, blastoids...
Abstract The curing reaction, structure, and glass transition behavior of epoxy‐clay nanocomposites prepared using several different resin/hardener ratios were investigated. Nonisothermal DSC experiments evidenced that the incorporation organoclay did not induce appreciable changes in enthalpy, but determined a slight acceleration without modifying activation energy. TEM SEM analyses nanocomposite resins showed presence micrometric aggregates for all investigated, even though these materials...
We describe here a method to obtain functional spinal and cranial motor neurons from human induced pluripotent stem cells (iPSCs). Direct conversion into neuron is obtained by ectopic expression of alternative modules transcription factors, namely Ngn2, Isl1 Lhx3 (NIL) or Phox2a (NIP). NIL NIP specify, respectively, identity. Our protocol starts with the generation modified iPSC lines in which are stably integrated genome via piggyBac transposon vector. Expression transgenes then doxycycline...
Abstract Background Anthrax and plague are diseases caused by Bacillus anthracis Yersinia pestis respectively. These bacteria etiological agents for worldwide zoonotic considered among the most feared potential bioterror agents. Strain differentiation is difficult these microorganisms because of their high intraspecies genome homogeneity. Moreover, fast strain identification comparison with known genotypes may be crucial naturally occurring outbreaks versus bioterrorist events...
Molecular genotyping of Bacillus anthracis, the etiologic agent anthrax, is important for differentiating and identifying strains from different geographic areas tracing deliberately released in a bioterrorism attack. We previously described multiple-locus variable-number tandem repeat (VNTR) analysis (MLVA) based on 25 marker loci. Although method has great power reproducibility, faster at low cost may be requested to accurately identify B. anthracis field.We used High Resolution Melter-1...
The Hippo pathway, a highly conserved signaling cascade that functions as an integrator of molecular signals and biophysical states, ultimately impinges upon the transcription coactivator Yes-associated protein 1 (YAP). Hippo-YAP has been shown to play key roles both at early embryonic stages implantation gastrulation, later during neurogenesis. To explore YAP's potential role in neurulation, we used self-organizing neuruloids grown from human stem cells on micropatterned substrates. We...
Abstract The statistical shape analysis called Procrustes minimizes the Frobenius distance between matrices by similarity transformations. method returns a set of optimal orthogonal matrices, which project each matrix into common space. This manuscript presents two types distances derived from for exploring between-matrices similarity. first one focuses on residuals analysis, i.e., residual-based metric. In contrast, second exploits fitted rotational-based Thanks to these distances,...
Abstract BACKGROUND: The reinforcement of elastomers by the addition fillers is one most important aspects in rubber science and technology. In order to optimise filler–polymer interface, innovative situ generation silica within isoprene was carried out means a bottom‐up approach through sol–gel process starting from tetraethoxysilane as precursor. main aim study effect concentration presence coupling agent on morphology dynamic mechanical behaviour composites. RESULTS: generated particles...
Protein conjugation with small ubiquitin-related modifier (SUMO) is a post-translational modification that modulates protein interactions and localisation. RANBP2 large nucleoporin endowed SUMO E3 ligase SUMO-stabilising activity, implicated in some cancer types. part of larger complex, consisting SUMO-modified RANGAP1, the GTP-hydrolysis activating factor for GTPase RAN. During mitosis, RANBP2-SUMO-RANGAP1 complex localises to mitotic spindle kinetochores after microtubule attachment. Here,...