A5043863243- Genomic variations and chromosomal abnormalities
- Yersinia bacterium, plague, ectoparasites research
- Bacteriophages and microbial interactions
- Bacillus and Francisella bacterial research
- Brucella: diagnosis, epidemiology, treatment
- Genomics and Chromatin Dynamics
- Burkholderia infections and melioidosis
- Chromosomal and Genetic Variations
- Mycobacterium research and diagnosis
- Genomics and Phylogenetic Studies
- Genetic Mapping and Diversity in Plants and Animals
- Salmonella and Campylobacter epidemiology
- RNA and protein synthesis mechanisms
- Antibiotic Resistance in Bacteria
- Microbial infections and disease research
- Tuberculosis Research and Epidemiology
- CRISPR and Genetic Engineering
- Escherichia coli research studies
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Genetics and Neurodevelopmental Disorders
- Identification and Quantification in Food
- Molecular Biology Techniques and Applications
- Bacterial Identification and Susceptibility Testing
- Vibrio bacteria research studies
- Vector-borne infectious diseases
Centre National de la Recherche Scientifique
2016-2025
CEA Paris-Saclay
2016-2025
Institut de Biologie Intégrative de la Cellule
2016-2025
Université Paris-Saclay
2016-2025
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2016-2025
Université Paris-Sud
2011-2020
École Nationale Supérieure de Techniques Avancées
1998-2016
Direction Générale de l'Armement
2013-2014
Centre Hospitalier d'Orsay
2011
Université Paris Cité
2009
Clustered regularly interspaced short palindromic repeats (CRISPRs) constitute a particular family of tandem found in wide range prokaryotic genomes (half eubacteria and almost all archaea). They consist succession highly conserved regions (DR) varying size from 23 to 47 bp, separated by similarly sized unique sequences (spacer) usually viral origin. A CRISPR cluster is flanked on one side an AT-rich sequence called the leader assumed be transcriptional promoter. Recent studies suggest that...
The remarkable repetitive elements called CRISPRs (clustered regularly interspaced short palindromic repeats) consist of repeats with non-repetitive or ‘spacers’. are present in both archaea and bacteria, association genes involved DNA recombination repair. In the Yersinia pestis genome, three such found at distinct loci, one them being highly polymorphic. authors have sequenced a total 109 alleles Y. they describe 29 new spacers, most specific to isolate. nine strains pseudotuberculosis ,...
CRISPR (clustered regularly interspaced short palindromic repeats) arrays and their associated (Cas) proteins confer bacteria archaea adaptive immunity against exogenous mobile genetic elements, such as phages or plasmids. CRISPRCasFinder allows the identification of both Cas proteins. The program includes: (i) an improved array detection tool facilitating expert validation based on a rating system, (ii) prediction orientation (iii) protein typing updated to match latest classification...
In Archeae and Bacteria, the repeated elements called CRISPRs for "clustered regularly interspaced short palindromic repeats" are believed to participate in defence against viruses. Short sequences spacers stored in-between elements. current model, motifs comprising repeats may target an invading DNA lead its degradation through a proposed mechanism similar RNA interference. Analysis of intra-species polymorphism shows that new (one spacer one element) added polarised fashion. Although their...
Abstract Background The classification of Brucella into species and biovars relies on phenotypic characteristics sometimes raises difficulties in the interpretation results due to an absence standardization typing reagents. In addition, resolution this biotyping is moderate requires manipulation living agent. More efficient DNA-based methods are needed, work explores suitability multiple locus variable number tandem repeats analysis (MLVA) for both identification. Results Eighty repeat loci...
Two Gram-negative, non-motile, non-spore-forming, coccoid bacteria (strains CCM 4915T and 4916), isolated from clinical specimens of the common vole Microtus arvalis during an epizootic in Czech Republic 2001, were subjected to a polyphasic taxonomic study. On basis 16S rRNA (rrs) recA gene sequence similarities, both isolates allocated genus Brucella. Affiliation Brucella was confirmed by DNA–DNA hybridization studies. Both strains reacted equally with M-monospecific antiserum lysed...
A Gram-negative, non-motile, non-spore-forming coccoid bacterium (strain BO1(T)) was isolated recently from a breast implant infection of 71-year-old female patient with clinical signs brucellosis. Affiliation strain BO1(T) to the genus Brucella confirmed by means polyamine pattern, polar lipid profile, fatty acid quinone system, DNA-DNA hybridization studies and insertion sequence 711 (IS711)-specific PCR. Strain harboured four five copies Brucella-specific element IS 711, displaying unique...
Two Gram-negative, non-motile, non-spore-forming coccoid bacteria (strains F8/08-60 T and F8/08-61) isolated from clinical specimens obtained baboons ( Papio spp.) that had delivered stillborn offspring were subjected to a polyphasic taxonomic study. On the basis of 16S rRNA gene sequence similarities, both strains, which possessed identical sequences, assigned genus Brucella . This placement was confirmed by extended multilocus analysis (MLSA), where strains whole-genome sequencing...
Two slow-growing, Gram-negative, non-motile, non-spore-forming, coccoid bacteria (strains F60T and F965), isolated in Austria from mandibular lymph nodes of two red foxes (Vulpes vulpes), were subjected to a polyphasic taxonomic analysis. In recent study, both isolates assigned the genus Brucella but could not be attributed any existing species. Hence, we have analysed strains further detail determine their exact position genetic relatedness other members Brucella. The genome sizes F965 3...
In Archaea and Bacteria, the arrays called CRISPRs for 'clustered regularly interspaced short palindromic repeats' CRISPR associated genes or cas provide adaptive immunity against viruses, plasmids transposable elements. Short sequences spacers, corresponding to fragments of invading DNA, are stored in-between repeated sequences. The CRISPR-Cas systems target homologous spacers leading their degradation. To facilitate investigations CRISPRs, we developed 12 years ago a website holding...
Some pathogenic bacteria are genetically very homogeneous, making strain discrimination difficult. In the last few years, tandem repeats have been increasingly recognized as markers of choice for genotyping a number pathogens. The rapid evolution these structures appears to contribute phenotypic flexibility availability whole-genome sequences has opened way systematic evaluation diversity and application epidemiological studies.This report presents database (http://minisatellites.u-psud.fr)...
Abstract Background The genome of Bacillus anthracis , the etiological agent anthrax, is highly monomorphic which makes differentiation between strains difficult. A Multiple Locus Variable-number tandem repeats (VNTR) Analysis (MLVA) assay based on 20 markers was previously described. It has considerable discrimination power, reproducibility, and low cost, especially since proposed can be typed by agarose-gel electrophoresis. However in an emergency situation, faster genotyping access to...
Yersinia pestis, the pathogen of plague, has greatly influenced human history on a global scale. Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR), an element participating in immunity against phages' invasion, is composed short repeated sequences separated by unique spacers and provides basis spoligotyping technology. In present research, three CRISPR loci were analyzed 125 strains Y. pestis from 26 natural plague foci China, former Soviet Union Mongolia analyzed, for...
Molecular and phylogeographic studies have led to the definition within Mycobacterium tuberculosis complex (MTBC) of a number geotypes ecotypes showing preferential geographic location or host preference. The MTBC is thought emerged in Africa, most likely Horn spread worldwide with human migrations. Under this assumption, there possibility that unknown deep branching lineages are present region. We genotyped by spoligotyping multiple locus variable tandem repeats (VNTR) analysis (MLVA) 435...
Currently available reference methods for the molecular epidemiology of Mycobacterium tuberculosis complex either lack sensitivity or are still too tedious and slow routine application. Recently, tandem repeat typing has emerged as a potential alternative. This report contributes to development M. by summarising existing data, developing additional markers, setting up freely accessible, fast, easy use, internet-based service strain identification. A collection 21 VNTRs incorporating 13...
Abstract Background Coxiella burnetii , the causative agent of Q fever, has a wide host range. Few epidemiological tools are available, and they often expensive or not easily standardized across laboratories. In this work, C. isolates from livestock ticks were typed using infrequent restriction site-PCR (IRS-PCR) multiple loci variable number tandem repeats (VNTR) analysis (MLVA). Results By applying IRS-PCR, 14 could be divided into six groups containing up to five different isolates....