A5074900332- Phytoplasmas and Hemiptera pathogens
- Insect symbiosis and bacterial influences
- Cocoa and Sweet Potato Agronomy
- Insect-Plant Interactions and Control
- Insect Resistance and Genetics
- Entomopathogenic Microorganisms in Pest Control
- Mosquito-borne diseases and control
- Vector-borne infectious diseases
- Plant Pathogenic Bacteria Studies
- Plant Virus Research Studies
- Vector-Borne Animal Diseases
- Studies on Chitinases and Chitosanases
- Viral Infections and Vectors
- Plant Parasitism and Resistance
- Legume Nitrogen Fixing Symbiosis
- Toxin Mechanisms and Immunotoxins
- Invertebrate Immune Response Mechanisms
- Rabies epidemiology and control
- Herpesvirus Infections and Treatments
- Insect behavior and control techniques
- Plant Pathogens and Resistance
- Nematode management and characterization studies
- Insect and Pesticide Research
- Yersinia bacterium, plague, ectoparasites research
- Genomics and Phylogenetic Studies
Biologie du Fruit et Pathologie
2014-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2018-2024
Université de Bordeaux
2008-2020
Institut National de la Recherche Agronomique
2003-2014
Laboratoire Génome et Développement des Plantes
2009-2010
Flavescence dorée (FD) is a European quarantine grapevine disease transmitted by the Deltocephalinae leafhopper Scaphoideus titanus. Whereas this vector had been introduced from North America, possible origin of FD phytoplasma needed to be challenged and correlated with ecological genetic drivers emergence. For that purpose, survey diversity these phytoplasmas in grapevines, S. titanus, black alders, alder leafhoppers clematis were conducted five countries. Out 132 map genotypes, only 11...
Abstract Background Coxiella burnetii , the causative agent of Q fever, has a wide host range. Few epidemiological tools are available, and they often expensive or not easily standardized across laboratories. In this work, C. isolates from livestock ticks were typed using infrequent restriction site-PCR (IRS-PCR) multiple loci variable number tandem repeats (VNTR) analysis (MLVA). Results By applying IRS-PCR, 14 could be divided into six groups containing up to five different isolates....
ou non, émanant des établissements d'enseignement et de recherche français étrangers, laboratoires publics privés.
ABSTRACT Phytoplasmas are uncultivated plant pathogens and cell wall-less bacteria transmitted from to by hemipteran insects. The phytoplasma's circulative propagative cycle in insects requires the crossing of midgut salivary glands, primary adhesion cells is an initial step toward invasion process. flavescence dorée (FD) phytoplasma possesses a set variable membrane proteins (Vmps) exposed on its surface, this pathogen suspected interact with insect cells. results showed that VmpA expressed...
ABSTRACT Phytoplasmas are plant-pathogenic bacteria transmitted by hemipteran insects. The leafhopper Euscelidius variegatus is a natural vector of chrysanthemum yellows phytoplasma (CYp) and laboratory flavescence dorée (FDp). two phytoplasmas induce different effects on this species: CYp slightly improves whereas FDp negatively affects insect fitness. To investigate the molecular bases these responses, transcriptome sequencing (RNA-seq) analysis E. infected with either or was performed....
Spiroplamas are helical, cell wall-less bacteria belonging to the Class Mollicutes, a group of microorganisms phylogenetically related low G+C, Gram-positive bacteria. Spiroplasma species all found associated with arthropods and few, including citri pathogenic plant. Thus S. has ability colonize cells two very distinct hosts, plant insect vector. While spiroplasmal factors involved in transmission by leafhopper Circulifer haematoceps have been identified, their specific contribution invasion...
Abstract The flavescence dorée phytoplasma undergoes a propagative cycle in its insect vectors by first interacting with the cell surfaces, primarily midgut lumen and subsequently salivary glands. Adhesion of to cells is mediated adhesin VmpA. We hypothesize that VmpA may have lectin-like activity, similar several adhesins bacteria invade gut. demonstrated luminal surface basal gland natural vector Scaphoideus titanus those experimental Euscelidius variegatus were differentially...
Abstract To perform its propagative and circulative cycle into insect vector, the flavescence dorée phytoplasma invades different cell types. Clathrin-mediated endocytosis is used by a wide range of bacteria to infect eukaryote cells. Among proteins interacting with adhesin VmpA, we identified adaptor protein complex AP-1 AP-2 suggesting that phytoplasmas could enter cells via clathrin-mediated endocytosis. By infection assays in culture, showed entry Drosophila S2 was more efficient than...
ABSTRACT Transmission of the phytopathogenic mollicutes, spiroplasmas, and phytoplasmas by their insect vectors mainly depends on ability to pass through gut cells, multiply in various tissues, traverse salivary gland cells. The passage these different barriers suggests molecular interactions between plant mollicute vector that regulate transmission. In present study, we focused interaction Spiroplasma citri its leafhopper vector, Circulifer haematoceps . An vitro protein overlay assay...
AbstractQ fever is caused by Coxiella burnetii, an organism widely found in nature and responsible for infections arthropods, pets, domestic wild animals, as well humans (1,2). Conventional diagnosis of Q mainly based on serological tests, such immunofluorescence, enzyme-linked immunosorbent assay, complement fixation (3). Isolation C. burnetii performed cell culture, animals or embryonated chicken eggs, however, the procedure time-consuming hazardous therefore restricted to specialized...
Spiroplasma citri is a plant pathogenic mollicute transmitted by the leafhopper vector Circulifer haematoceps. Successful transmission requires spiroplasmas to cross intestinal epithelium and salivary gland barriers through endocytosis mediated receptor-ligand interactions. To characterize these interactions we studied adhesion invasion capabilities of S. mutant using Ciha-1 cell line. GII3 wild-type contains 7 plasmids, 5 which (pSci1 5) encode 8 related adhesins (ScARPs). As compared...
Flavescence dorée (FD) of grapevine is a phloem bacterial disease that threatens European vineyards. The associated with non-cultivable mollicute, phytoplasma transmitted by the leafhopper Scaphoideus titanus in persistent, propagative manner. specificity insect transmission presumably mediated through interactions between host tissues and surface proteins comprising so-called variable membrane (Vmps). Plant spiroplasmas phytoplasmas share same ecological niches, sieve elements plants...
Background Spiroplasma citri is a wall-less bacterium that colonizes phloem vessels of large number host plants. Leafhopper vectors transmit S. in propagative and circulative manner, involving colonization multiplication bacteria various insect organs. Previously we reported phosphoglycerate kinase (PGK), the well-known glycolytic enzyme, bound to leafhopper actin was unexpectedly implicated internalization process into Circulifer haematoceps cells. Methodology/Principal Findings In an...
Spiroplasma citri strain GII3 contains seven plasmids, pSciA and pSci1–6, that share extensive regions of sequence homology display a mosaic gene organization. Plasmid pSci2 comprises 12 coding sequences (CDS), three which encode polypeptides homologous to proteins Soj/ParA, involved in chromosome partitioning, TrsE Mob/TraG, implicated the type IV secretion pathway. One CDS encodes adhesin-like protein ScARP3d whereas other eight with no known proteins. The pE soj have counterparts all...
Introduction The adhesion of flavescence dorée phytoplasma to the midgut epithelium cells their insect vectors is partially mediated by variable membrane protein A (VmpA), an adhesin which shows lectin properties. In order identify receptor for VmpA, we identified Euscelidius variegatus cell proteins interacting with recombinant VmpA-His 6 . Methods E. were mass spectrometry analysis VmpA- complexes formed upon in vitro interaction assays. To assess impact VmpA binding, reduced expression...
Spiroplasma citri is a cell wall-less, plant pathogenic bacteria that colonizes two distinct hosts, the leafhopper vector and host plant. Given absence of wall, surface proteins including lipoproteins transmembrane polypeptides are expected to play key roles in spiroplasma/host interactions. Important functions spiroplasma/insect interactions have been shown for few such as major lipoprotein spiralin, S. adhesion-related (ScARPs) sugar transporter subunit Sc76. efficient transmission from...
Successful transmission of Spiroplasma citri by its leafhopper vector requires a specific interaction between the spiroplasma surface and insect cells. With aim studying these interactions at cellular molecular levels, cell line, named Ciha-1, was established using embryonic tissues from eggs S. natural Circulifer haematoceps. This is first report, to our knowledge, line for this species successful infection insect-transmissible strain GII3. Adherence spiroplasmas cultured Ciha-1 cells...
ou non, émanant des établissements d'enseignement et de recherche français étrangers, laboratoires publics privés.