A5025826529- Phytoplasmas and Hemiptera pathogens
- Mycorrhizal Fungi and Plant Interactions
- Insect symbiosis and bacterial influences
- Fungal Biology and Applications
- Insect-Plant Interactions and Control
- Insect Resistance and Genetics
- Plant Virus Research Studies
- Horticultural and Viticultural Research
- Plant-Microbe Interactions and Immunity
- Plant Pathogenic Bacteria Studies
- Fungal and yeast genetics research
- Cocoa and Sweet Potato Agronomy
- Lichen and fungal ecology
- Allergic Rhinitis and Sensitization
- Heavy metals in environment
- Plant Pathogens and Fungal Diseases
- Food Allergy and Anaphylaxis Research
- Plant nutrient uptake and metabolism
- Fermentation and Sensory Analysis
- Plant Physiology and Cultivation Studies
- Animal Disease Management and Epidemiology
- Nuts composition and effects
- Animal Virus Infections Studies
- Plant and Biological Electrophysiology Studies
- Research on scale insects
National Research Council
2015-2024
Institute for Sustainable Plant Protection
2015-2024
Centro di Ricerca per la Patologia Vegetale
2006-2013
University of Turin
2004-2012
Université de Lorraine
2002
Université de Dschang
1996
Abstract Background Translational and post-translational protein modifications play a key role in the response of plants to pathogen infection. Among latter, phosphorylation is critical modulating structure, localization interaction with other partners. In this work, we used multiplex staining approach 2D gels study quantitative changes proteome phosphoproteome Flavescence dorée-affected recovered ‘Barbera’ grapevines, compared healthy plants. Results We identified 48 proteins that...
Flavescence dorée, a threatening disease of grapevine caused by FD phytoplasma (FDp), is distributed within the most important wine-producing areas Europe and has severe effects on both vineyard productivity landscape management. FDp quarantine pest in Europe, despite efforts to contain pathogen, still spreading. In this work, new genetic markers for fine characterization at local scale are presented. Our findings improve knowledge epidemiological cycle offer possibility tracking route...
ABSTRACT Phytoplasmas are plant-pathogenic bacteria transmitted by hemipteran insects. The leafhopper Euscelidius variegatus is a natural vector of chrysanthemum yellows phytoplasma (CYp) and laboratory flavescence dorée (FDp). two phytoplasmas induce different effects on this species: CYp slightly improves whereas FDp negatively affects insect fitness. To investigate the molecular bases these responses, transcriptome sequencing (RNA-seq) analysis E. infected with either or was performed....
ABSTRACT The transition from vegetative mycelium to fruit body in truffles requires differentiation processes which lead edible bodies (ascomata) consisting of different cell and tissue types. identification genes differentially expressed during these developmental can contribute greatly a better understanding truffle morphogenesis. A cDNA library was constructed RNAs the white Tuber borchii , 214 cDNAs were sequenced. Up 58% sequence tags corresponded known genes. majority identified...
The genome sequences of mycorrhizal fungi will provide new opportunities for studying the biology and evolution underlying this symbiotic lifestyle. generation null mutants at wild-type loci is one best methods gene-function assignment in post-genomic era. To our knowledge, superoxide dismutase 1 (SOD1)-null ericoid fungus Oidiodendron maius first example a gene-targeted disruption via homologous recombination fungus. OmSOD1 by Agrobacterium-mediated transformation resulted presence...
Two bacterial symbionts of the European pest leafhopper, Macrosteles quadripunctulatus (Hemiptera: Cicadellidae), were fully sequenced. "Candidatus Sulcia muelleri" and "Ca. Nasuia deltocephalinicola" represent two smallest known genomes at 190 kb 112 kb, respectively. Genome sequences are nearly identical to strains reported from closely related host species, M. quadrilineatus.
Molecular changes associated with response to powdery mildew (PM) caused by Erysiphe necator have been largely explored in Vitis vinifera cultivars, but little is known on transcriptional and metabolic modifications following application of resistance elicitors against this disease. In study, the whole transcriptome sequencing, hormone metabolite analyses were combined dissect long-term defense mechanisms induced molecular reprogramming events PM-infected ‘Moscato’ ‘Nebbiolo’ leaves treated...
The phytoplasma-borne disease flavescence dorée is still a threat to European viticulture, despite mandatory control measures and prophylaxis against the leafhopper vector. Given economic importance of grapevine, it essential find alternative strategies contain spread, in order possibly reduce current use harmful insecticides. Further studies pathogen, vector mechanisms phytoplasma-host interactions could improve our understanding disease. In this work, RNA-Seq technology followed by three...
ABSTRACT To highlight different transcriptional behaviors of the phytoplasma in plant and animal host, expression 14 genes “ Candidatus Phytoplasma asteris,” chrysanthemum yellows strain, was investigated at times following infection a host ( Arabidopsis thaliana ) two insect vector species Macrosteles quadripunctulatus Euscelidius variegatus ). Target were selected among those encoding antigenic membrane proteins, transporters, secreted general enzymes. Transcripts detected for all analyzed...
Micro(mi)RNAs play crucial roles in plant developmental processes and defense responses to biotic abiotic stresses. In the last years, many works on small RNAs grapevine (Vitis spp.) were published, several conserved putative novel grapevine-specific miRNAs identified. order reorganize high quantity of available data, we produced "miRVIT," first database all miRNA candidates characterized so far, still not deposited miRBase. To this aim, each accession was renamed, repositioned version...
In recent years, there has been a growing global health concern, necessitating the search for potent, resistance-free medicinal plants with antibacterial properties. This study evaluates phytochemical and properties of Vernonia amygdalina (bitter leaf) extracts as an alternative treatment bacterial infections. Ethanolic aqueous V. leaves were prepared subjected to screening, revealing presence quinones, tannins, steroids, saponins, terpenoids, alkaloids, phenols, flavonoids. The activity was...
Abstract Background The expressed sequence tag M6G10 was originally isolated from a screening for differentially transcripts during the reproductive stage of white truffle Tuber borchii . mRNA levels increased dramatically fruiting body maturation compared to vegetative mycelial stage. Results Bioinformatics tools, phylogenetic analysis and expression studies were used support hypothesis that this sequence, named TbDHN1, is first dehydrin (DHN)-like coding gene in fungi. Homologs gene, all...
The leafhopper Scaphoideus titanus is the primary vector of Flavescence dorée phytoplasma (FDp) in European vineyards. one most severely damaging diseases Vitis vinifera and, consequently, a major threat to grape and wine production several countries. Control measures are compulsory, but they mainly involve large-scale insecticide treatments, with detrimental impacts on environment. One possible solution exploit largely unexplored genetic diversity viruses infecting S. as highly specific...
Hazelnut allergy, which is characterized by symptoms that range from mild to severe, one of the most common allergies in children throughout Europe, and an accurate diagnosis this allergy therefore essential. However, lipophilic allergens, such as oleosins, are generally underrepresented diagnostic tests. We sought characterize IgE reactivity raw roasted hazelnut using sera hazelnut-allergic pediatric patients.Raw oil body-associated proteins were analyzed means 1D 2D electrophoresis MS....
Cadmium is a genotoxic pollutant known to target proteins that are involved in DNA repair and antioxidant defence, altering their functions ultimately causing mutagenic carcinogenic effects. We have identified PLAC8 domain-containing protein, named OmFCR, by yeast functional screen aimed at identifying genes cadmium resistance the endomycorrhizal fungus Oidiodendron maius. OmFCR shows remarkable specificity mediating resistance. Both its function nuclear localization strictly depend on...
Halyomorpha halys (Stål), the brown marmorated stink bug, is a highly invasive insect species that causes significant agricultural losses, especially to orchard fruits, vegetables, herbaceous and ornamental plants. It also nuisance pest seeks shelter in indoor spaces during winter months. Harnessing H. virome can result new environmentally sustainable approaches contain its populations relatated damages. In this study, RNA-Seq data were used explore associated ten field collected Lombardy...
ABSTRACT cDNA arrays were used to explore mechanisms controlling fruiting body development in the truffle Tuber borchii. Differences gene expression higher between reproductive and vegetative stage than two stages of maturation. We suggest hypotheses about importance various physiological processes during bodies.